Human Phospholipase B1 ELISA Kit | PLB1 elisa kit
Human Phospholipase B1, membrane-associated (PLB1) ELISA Kit
Gene Names
PLB1; PLB; PLB/LIP
Reactivity
Human
Synonyms
Phospholipase B1; Human Phospholipase B1; membrane-associated (PLB1) ELISA Kit; PLB1 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human PLB1. No significant cross-reactivity or interference between Human PLB1 and analogues was observed.
Sequence Length
1458
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for PLB1 elisa kit
By Western blot analysis using an antibody against guinea pig phospholipase B (PLB), Maury et al. (2002) detected a 97-kD polypeptide in the soluble and membrane fractions of human epidermis that was enriched in the soluble fraction. Consistent with these results, they found that PLB activity was greatest in the soluble fraction.
Trypsin had no effect on the molecular mass or enzymatic activity, suggesting that the protein they detected was already in an active form.
Immunolocalization experiments revealed that PLB is expressed in the entire human epidermis with an accumulation in the dermoepidermis junction. In situ hybridization also indicated expression in the entire epidermis.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PLB1 in samples. An antibody specific for PLB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLB1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Trypsin had no effect on the molecular mass or enzymatic activity, suggesting that the protein they detected was already in an active form.
Immunolocalization experiments revealed that PLB is expressed in the entire human epidermis with an accumulation in the dermoepidermis junction. In situ hybridization also indicated expression in the entire epidermis.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PLB1 in samples. An antibody specific for PLB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLB1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47,341 Da
NCBI Official Full Name
phospholipase B1, membrane-associated isoform 2
NCBI Official Synonym Full Names
phospholipase B1
NCBI Official Symbol
PLB1
NCBI Official Synonym Symbols
PLB; PLB/LIP
NCBI Protein Information
phospholipase B1, membrane-associated
UniProt Protein Name
Phospholipase B1, membrane-associated
UniProt Gene Name
PLB1
UniProt Synonym Gene Names
PLB; Phospholipase B; hPLB; PLB/LIP
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Product Notes
The Human PLB1 plb1 (Catalog #AAA13202) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA13202 ELISA Kit recognizes Human PLB1. It is sometimes possible for the material contained within the vial of "Phospholipase B1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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