Human Angiostatin K1-3 ELISA Kit | PLG elisa kit

Human Angiostatin K1-3 PicoKine Fast ELISA Kit

Cat. Num. AAA125350

• Reactivity: Human
• Synonyms: Angiostatin K1-3; N/A; Human Angiostatin K1-3 PicoKine Fast ELISA Kit; Human Angiostatin K1-3; Plasminogen; 3.4.21.7; Plasmin heavy chain A; Activation peptide; Angiostatin; Plasmin heavy chain A, short form; Plasmin light chain B; PLG; PLG elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human PLG
• Sequence Length: 810
• Samples: Cell culture supernates, serum and plasma(heparin, EDTA)
• Detection Range: 156pg/ml-10000pg/ml
• Sensitivity: <10pg/ml

• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Standard Curve (Sample)

Related Product Information for PLG elisa kit

Principle of the Assay: The Picokine™ Human PLG Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human PLG with a 96-well strip plate that is pre-coated with antibody specific for PLG. The detection antibody is a biotinylated antibody specific for PLG. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human PLG with immunogen: Expression system for standard: NSO,V98-S355(K1-3). The kit is analytically validated with ready to use reagents. To measure Human PLG, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human PLG in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human PLG in the sample.!!Background/Introduction: Ang K1-3 is a single, non-glycosylated polypeptide chain containing 259 amino acids. It represents a proteolytic fragment of plasminogen containing the first three kringle structures. Ang K1-3 reduces endothelial cell proliferation and acts as a potent inhibitor of angiogenesis and tumor growth. It displays increased inhibitory activity(ED?? =70 nM) relative to kringles 1-4(ED?? = 135 nM).

NCBI and Uniprot Product Information

• NCBI GI #: 4505881
• NCBI GeneID: 5340
• NCBI Accession #: NP_000292.1
• NCBI GenBank Nucleotide #: NP_000292.1
• UniProt Accession #: P00747
• NCBI Official Full Name: plasminogen isoform 1
• NCBI Official Synonym Full Names: plasminogen
• NCBI Official Symbol: PLG
• NCBI Protein Information: plasminogen
• UniProt Protein Name: Plasminogen
• UniProt Gene Name: PLG
• UniProt Entry Name: PLMN_HUMAN