Standard Curve (Sample)
(Human Angiostatin K1-3 PicoKine ELISA Kit standard curve)
Human Angiostatin K1-3 ELISA Kit | PLG elisa kit
Human Angiostatin K1-3 PicoKine ELISA Kit
Reactivity
Human
Synonyms
Angiostatin K1-3; N/A; Human Angiostatin K1-3 PicoKine ELISA Kit; Plasminogen; plasminogen; Ang K1-3; PLG elisa kit
Reactivity
Human
Specificity
Natural and recombinant human Angiostatin K1-3
Sequence Length
810
Samples
Serum, plasma and cell culture supernates
Assay Type
Sandwich
Detection Range
156pg/ml-10,000pg/ml
Sensitivity
< 50pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
(Human Angiostatin K1-3 PicoKine ELISA Kit standard curve)
Standard Curve (Sample)
(Human Angiostatin K1-3 PicoKine ELISA Kit standard curve)
Related Product Information for PLG elisa kit
Principle of the assay: human Angiostatin K1-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Angiostatin K1-3 has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Angiostatin K1-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Angiostatin K1-3 amount of sample captured in plate.
Background: Ang K1-3 is a single, non-glycosylated polypeptide chain containing 259 amino acids. It represents a proteolytic fragment of plasminogen containing the first three kringle structures. Ang K1-3 reduces endothelial cell proliferation and acts as a potent inhibitor of angiogenesis and tumor growth. It displays increased inhibitory activity (ED50 =70 nM) relative to kringles 1-4 (ED50 = 135 nM).
Background: Ang K1-3 is a single, non-glycosylated polypeptide chain containing 259 amino acids. It represents a proteolytic fragment of plasminogen containing the first three kringle structures. Ang K1-3 reduces endothelial cell proliferation and acts as a potent inhibitor of angiogenesis and tumor growth. It displays increased inhibitory activity (ED50 =70 nM) relative to kringles 1-4 (ED50 = 135 nM).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
90,569 Da
NCBI Official Full Name
Plasminogen
NCBI Official Synonym Full Names
plasminogen
NCBI Official Symbol
PLG
NCBI Protein Information
plasminogen
UniProt Protein Name
Plasminogen
UniProt Gene Name
PLG
UniProt Entry Name
PLMN_HUMAN
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Product Notes
The Human PLG plg (Catalog #AAA45299) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA45299 ELISA Kit recognizes Human PLG. It is sometimes possible for the material contained within the vial of "Angiostatin K1-3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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