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▼Polyclonal Antibodies
At AAA Biotech also known as AAA Bio or AAABio, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
Viewing 2850-2900 of 96812 product results
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of CACO-2 cells using anti-MKP-1/DUSP1 antibody (AAA125646).Overlay histogram showing CACO-2 cells stained with AAA125646 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MKP-1/DUSP1 Antibody (AAA125646, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
MKP-1/DUSP1, Polyclonal Antibody (Cat# AAA125646)
Full Name
Anti-MKP-1/DUSP1 Antibody
Gene Names
DUSP1; HVH1; MKP1; CL100; MKP-1; PTPN10
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of CACO-2 cells using anti-HSD17B2 antibody (AAA125658).Overlay histogram showing CACO-2 cells stained with AAA125658 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HSD17B2 Antibody (AAA125658, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
HSD17B2, Polyclonal Antibody (Cat# AAA125658)
Full Name
Anti-HSD17B2 Antibody
Gene Names
HSD17B2; HSD17; SDR9C2; EDH17B2
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of A431 cells using anti-KIBRA/WWC1 antibody (AAA125673).Overlay histogram showing A431 cells stained with AAA125673 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KIBRA/WWC1 Antibody (AAA125673, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
KIBRA/WWC1, Polyclonal Antibody (Cat# AAA125673)
Full Name
Anti-KIBRA/WWC1 Antibody
Gene Names
WWC1; KIBRA; HBEBP3; HBEBP36; MEMRYQTL; PPP1R168
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 4. Flow Cytometry analysis of U87 cells using anti-GSDMD antibody (AAA125674).Overlay histogram showing U87 cells stained with AAA125674 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GSDMD Antibody (AAA125674,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
GSDMD, Polyclonal Antibody (Cat# AAA125674)
Full Name
Anti-GSDMD Antibody
Gene Names
GSDMD; DF5L; DFNA5L; FKSG10; GSDMDC1
Reactivity
Human
Applications
Direct ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(Figure 5. IHC analysis of Golm1/GOLPH2 using anti-Golm1/GOLPH2 antibody (AAA125681).Golm1/GOLPH2 was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Golm1/GOLPH2 Antibody (AAA125681) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Golm1/GOLPH2, Polyclonal Antibody (Cat# AAA125681)
Full Name
Anti-Golm1/GOLPH2 Antibody
Gene Names
Golm1; GP73; Golph2; AW125446; PSEC0257; 2310001L02Rik; D030064E01Rik
Reactivity
Mouse, Rat
Applications
Direct ELISA, Immunofluorescence, Immunocytochemistry, Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of PC-3 cells using anti-MRP5/ABCC5 antibody (AAA125683).Overlay histogram showing PC-3 cells stained with AAA125683 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MRP5/ABCC5 Antibody (AAA125683, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
MRP5/ABCC5, Polyclonal Antibody (Cat# AAA125683)
Full Name
Anti-MRP5/ABCC5 Antibody
Gene Names
ABCC5; MRP5; SMRP; ABC33; MOATC; MOAT-C; pABC11; EST277145
Reactivity
Human
Applications
Direct ELISA, Flow Cytometry, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 5. Flow Cytometry analysis of PC-3 cells using anti-NOX5 antibody (AAA125684).Overlay histogram showing PC-3 cells stained with AAA125684 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NOX5 Antibody (AAA125684,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
NOX5, Polyclonal Antibody (Cat# AAA125684)
Full Name
Anti-NOX5 Antibody
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
CD39/ENTPD1, Polyclonal Antibody (Cat# AAA125685)
Full Name
Anti-CD39/ENTPD1 Antibody
Gene Names
ENTPD1; CD39; ATPDase; NTPDase-1
Reactivity
Human
Applications
Direct ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemisry)
(Figure 3. IHC analysis of MUC20 using anti-MUC20 antibody.MUC20 was detected in paraffin-embedded section of mouse kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MUC20 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
MUC20, Polyclonal Antibody (Cat# AAA125170)
Full Name
Anti-MUC20 Antibody
Gene Names
MUC20; MUC-20
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Pricing
FCM/FACS (Flow Cytometry)
(Figure 4. Flow Cytometry analysis of JK cells using anti-NCLN antibody (AAA127750).Overlay histogram showing JK cells stained with AAA127750 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NCLN Antibody (AAA127750, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
NCLN, Polyclonal Antibody (Cat# AAA127750)
Full Name
Anti-NCLN Antibody Picoband
Gene Names
NCLN; NET59
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of HepG2 cells using anti-PHYHD1 antibody (AAA127776).Overlay histogram showing HepG2 cells stained with AAA127776 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PHYHD1 Antibody (AAA127776, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
PHYHD1, Polyclonal Antibody (Cat# AAA127776)
Full Name
Anti-PHYHD1 Antibody Picoband
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of U20S cells using anti-RUFY2 antibody (AAA127781).Overlay histogram showing U20S cells stained with AAA127781 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RUFY2 Antibody (AAA127781, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
RUFY2, Polyclonal Antibody (Cat# AAA127781)
Full Name
Anti-RUFY2 Antibody Picoband
Gene Names
RUFY2; RABIP4R; ZFYVE13
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 5. Flow Cytometry analysis of RT4 cells using anti-NUDCD3 antibody (AAA127782).Overlay histogram showing RT4 cells stained with AAA127782 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NUDCD3 Antibody (AAA127782, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
NUDCD3, Polyclonal Antibody (Cat# AAA127782)
Full Name
Anti-NUDCD3 Antibody Picoband
Gene Names
NUDCD3; NudCL
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 5. Flow Cytometry analysis of SH-SY5Y cells using anti-PCDHB14 antibody (AAA127785).Overlay histogram showing SH-SY5Y cells stained with AAA127785 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PCDHB14 Antibody (AAA127785, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PCDHB14, Polyclonal Antibody (Cat# AAA127785)
Full Name
Anti-PCDHB14 Antibody Picoband
Gene Names
PCDHB14; PCDH-BETA14
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of U251 cells using anti-SYT16 antibody (AAA127787).Overlay histogram showing U251 cells stained with AAA127787 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SYT16 Antibody (AAA127787, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
SYT16, Polyclonal Antibody (Cat# AAA127787)
Full Name
Anti-SYT16 Antibody Picoband
Gene Names
SYT16; yt14r; SYT14L; syt14r; Strep14; CHR14SYT
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of 293T cells using anti-RILPL1 antibody (AAA127788).Overlay histogram showing 293T cells stained with AAA127788 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RILPL1 Antibody (AAA127788, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
RILPL1, Polyclonal Antibody (Cat# AAA127788)
Full Name
Anti-RILPL1 Antibody Picoband
Gene Names
RILPL1; RLP1; GOSPEL
Reactivity
Human, Rat
Applications
Western Blot
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 2. IF analysis of HSPA12A using anti-HSPA12A antibody (AAA127795).HSPA12A was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-HSPA12A Antibody (AAA127795) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
HSPA12A, Polyclonal Antibody (Cat# AAA127795)
Full Name
Anti-HSPA12A Antibody Picoband
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of RT4 cells using anti-PRSS22 antibody (AAA127799).Overlay histogram showing RT4 cells stained with AAA127799 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PRSS22 Antibody (AAA127799, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PRSS22, Polyclonal Antibody (Cat# AAA127799)
Full Name
Anti-PRSS22 Antibody Picoband
Gene Names
PRSS22; BSSP-4; SP001LA; hBSSP-4
Reactivity
Human
Applications
Western Blot, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of PC-3 cells using anti-PCDHA6 antibody (AAA127808).Overlay histogram showing PC-3 cells stained with AAA127808 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PCDHA6 Antibody (AAA127808, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PCDHA6, Polyclonal Antibody (Cat# AAA127808)
Full Name
Anti-PCDHA6 Antibody Picoband
Gene Names
PCDHA6; CNR2; CNRN2; CNRS2; CRNR2; PCDH-ALPHA6
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 2. IF analysis of IQCE using anti-IQCE antibody (AAA127811) and anti-Beta Tubulin antibody (M01857-3).IQCE was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-IQCE Antibody (AAA127811) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4 degree C. DyLight488 Conjugated Donkey Anti-Rabbit IgG and DyLight594 Conjugated Goat Anti-Mouse IgG (BA1141) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
IQCE, Polyclonal Antibody (Cat# AAA127811)
Full Name
Anti-IQCE Antibody Picoband
Gene Names
IQCE; 1700028P05Rik
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of JK cells using anti-PIANP antibody (AAA127812).Overlay histogram showing JK cells stained with AAA127812 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PIANP Antibody (AAA127812, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PIANP, Polyclonal Antibody (Cat# AAA127812)
Full Name
Anti-PIANP Antibody Picoband
Gene Names
PIANP; PANP; leda-1; C12orf53
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of U20S cells using anti-RUNDC1 antibody (AAA127813).Overlay histogram showing U20S cells stained with AAA127813 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RUNDC1 Antibody (AAA127813, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
RUNDC1, Polyclonal Antibody (Cat# AAA127813)
Full Name
Anti-RUNDC1 Antibody Picoband
Reactivity
Human, Monkey
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of JK cells using anti-NSMCE4A antibody (AAA127827).Overlay histogram showing JK cells stained with AAA127827 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-NSMCE4A Antibody (AAA127827, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
NSMCE4A, Polyclonal Antibody (Cat# AAA127827)
Full Name
Anti-NSMCE4A Antibody Picoband
Gene Names
NSMCE4A; NS4EA; NSE4A; C10orf86
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of K562 cells using anti-SAMD1 antibody (AAA127831).Overlay histogram showing K562 cells stained with AAA127831 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SAMD1 Antibody (AAA127831, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
SAMD1, Polyclonal Antibody (Cat# AAA127831)
Full Name
Anti-SAMD1 Antibody Picoband
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 4. Flow Cytometry analysis of JK cells using anti-RBM12B antibody (AAA127839).Overlay histogram showing JK cells stained with AAA127839 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RBM12B Antibody (AAA127839, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
RBM12B, Polyclonal Antibody (Cat# AAA127839)
Full Name
Anti-RBM12B Antibody Picoband
Gene Names
RBM12B; MGC:33837
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 4. Flow Cytometry analysis of 293T cells using anti-SURF2 antibody (AAA127842).Overlay histogram showing 293T cells stained with AAA127842 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SURF2 Antibody (AAA127842, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
SURF2, Polyclonal Antibody (Cat# AAA127842)
Full Name
Anti-SURF2 Antibody Picoband
Gene Names
SURF2; SURF-2
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 4. Flow Cytometry analysis of PC-3 cells using anti-SPAG7 antibody (AAA127848).Overlay histogram showing PC-3 cells stained with AAA127848 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SPAG7 Antibody (AAA127848, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
SPAG7, Polyclonal Antibody (Cat# AAA127848)
Full Name
Anti-SPAG7 Antibody Picoband
Gene Names
SPAG7; ACRP; FSA-1
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 2. IF analysis of LAGE3 using anti-LAGE3 antibody (AAA127857) and anti-Beta Tubulin antibody (M01857-3).LAGE3 was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-LAGE3 Antibody (AAA127857) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
LAGE3, Polyclonal Antibody (Cat# AAA127857)
Full Name
Anti-LAGE3 Antibody Picoband
Gene Names
LAGE3; CVG5; ESO3; ITBA2; DXS9879E; DXS9951E
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 4. Flow Cytometry analysis of SiHa cells using anti-RRP7A antibody (AAA127866).Overlay histogram showing SiHa cells stained with AAA127866 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RRP7A Antibody (AAA127866, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
RRP7A, Polyclonal Antibody (Cat# AAA127866)
Full Name
Anti-RRP7A Antibody Picoband
Gene Names
RRP7A; CGI-96; BK126B4.3
Reactivity
Human, Monkey
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of Raji cells using anti-PRPF39 antibody (AAA127870).Overlay histogram showing Raji cells stained with AAA127870 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRPF39 Antibody (AAA127870, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PRPF39, Polyclonal Antibody (Cat# AAA127870)
Full Name
Anti-PRPF39 Antibody Picoband
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of 293T cells using anti-NOC4L antibody (AAA127873).Overlay histogram showing 293T cells stained with AAA127873 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NOC4L Antibody (AAA127873, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
NOC4L, Polyclonal Antibody (Cat# AAA127873)
Full Name
Anti-NOC4L Antibody Picoband
Gene Names
NOC4L; NOC4; NET49; UTP19
Reactivity
Human
Applications
Flow Cytometry, Western Blot, Immunofluorescence, Immunocytochemistry
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 4. Flow Cytometry analysis of JK cells using anti-MYL5 antibody (AAA127876).Overlay histogram showing JK cells stained with AAA127876 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MYL5 Antibody (AAA127876, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
MYL5, Polyclonal Antibody (Cat# AAA127876)
Full Name
Anti-MYL5 Antibody Picoband
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Western Blot, Immunocytochemistry, Immunohistochemistry, Immunofluorescence
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 5. Flow Cytometry analysis of HepG2 cells using anti-MAGED4 antibody (AAA127895).Overlay histogram showing HepG2 cells stained with AAA127895 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MAGED4 Antibody (AAA127895, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
MAGED4, Polyclonal Antibody (Cat# AAA127895)
Full Name
Anti-MAGED4 Antibody Picoband
Gene Names
MAGED4; MAGE1; MAGEE1; MAGE-E1
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of MCF-7 cells using anti-PPM1N antibody (AAA127902).Overlay histogram showing MCF-7 cells stained with AAA127902 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPM1N Antibody (AAA127902, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PPM1N, Polyclonal Antibody (Cat# AAA127902)
Full Name
Anti-PPM1N Antibody Picoband
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of HEL cells using anti-ITPRIPL1 antibody (AAA127911).Overlay histogram showing HEL cells stained with AAA127911 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ITPRIPL1 Antibody (AAA127911, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
ITPRIPL1, Polyclonal Antibody (Cat# AAA127911)
Full Name
Anti-ITPRIPL1 Antibody Picoband
Gene Names
ITPRIPL1; KIAA1754L
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of THP-1 cells using anti-NXPE3 antibody (AAA127918).Overlay histogram showing THP-1 cells stained with AAA127918 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-NXPE3 Antibody (AAA127918, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
NXPE3, Polyclonal Antibody (Cat# AAA127918)
Full Name
Anti-NXPE3 Antibody Picoband
Gene Names
NXPE3; FAM55C; MST115; MSTP115
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(Figure 4. IHC analysis of CD14 using anti-CD14 antibody (AAA124550).CD14 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD14 Antibody (AAA124550) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
CD14, Polyclonal Antibody (Cat# AAA124550)
Full Name
Anti-CD14 Picoband Antibody
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified
Pricing
IHC (Immunohiostchemistry)
(Figure 2. IHC analysis of CD80 using anti-CD80 antibody (AAA124556).CD80 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD80 Antibody (AAA124556) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
CD80, Polyclonal Antibody (Cat# AAA124556)
Full Name
Anti-CD80 antibody
Gene Names
CD80; B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1
Reactivity
Human, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Immunohistochemistry
Pricing
IHC (Immunohistochemistry)
(Figure 4. IHC analysis of BRG1 using anti-BRG1 antibody (AAA124557).BRG1 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BRG1 Antibody (AAA124557) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
BRG1, Polyclonal Antibody (Cat# AAA124557)
Full Name
Anti-BRG1 Picoband antibody
Gene Names
SMARCA4; BRG1; CSS4; SNF2; SWI2; MRD16; RTPS2; BAF190; SNF2L4; SNF2LB; hSNF2b; BAF190A
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot, Immunohistochemistry
Pricing
IHC (Immunohiostchemistry)
(Figure 3. IHC analysis of ELAVL2 HuB using anti-ELAVL2 HuB antibody.ELAVL2 HuB was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ELAVL2 HuB antibody.ELAVL2 HuB was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ELAVL2 HuB antibody.ELAVL2 HuB was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ELAVL2 HuB antibody.ELAVL2 HuB was detected in paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ELAVL2 HuB Antibody overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
ELAVL2 HuB, Polyclonal Antibody (Cat# AAA124965)
Full Name
Anti-ELAVL2 HuB Antibody
Gene Names
ELAVL2; HUB; HELN1; HEL-N1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Pricing
IHC (Immunohiostchemistry)
(Figure 3. IHC analysis of RASAL1 using anti-RASAL1 antibody.RASAL1 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-RASAL1 antibody.RASAL1 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-RASAL1 antibody.RASAL1 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-RASAL1 antibody.RASAL1 was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-RASAL1 antibody.RASAL1 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-RASAL1 Antibody overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
RASAL1/RASAL1, Polyclonal Antibody (Cat# AAA124966)
Full Name
Anti-RASAL1/RASAL1 Antibody
Gene Names
RASAL1; RASAL
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Pricing
IHC (Immunohiostchemistry)
(Figure 3. IHC analysis of IL36 alpha using anti-IL36 alpha antibody.IL36 alpha was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/mL rabbit anti-IL36 alpha antibody.IL36 alpha was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/mL rabbit anti-IL36 alpha Antibody overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
IL36 alpha/IL36A, Polyclonal Antibody (Cat# AAA124967)
Full Name
Anti-IL36 alpha/IL36A Antibody
Gene Names
IL36A; FIL1; FIL1E; IL1F6; IL-1F6; IL1(EPSILON); FIL1(EPSILON)
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Pricing
WB (Western Blot)
(Figure 3. Western blot analysis of CD7 using anti-CD7 antibody (AAA124676).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.Lane 1: recombinant human CD7 protein 1ng.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD7 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD7 at approximately 30-40KD. The expected band size for CD7 is at 18KD.)
CD7, Polyclonal Antibody (Cat# AAA124676)
Full Name
Anti-CD7 Picoband antibody
Gene Names
CD7; GP40; TP41; Tp40; LEU-9
Reactivity
Human
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of Alpha 1 microglobulin using anti-Alpha 1 microglobulin antibody (AAA124687).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat liver tissue lysates,Lane 2: mouse liver tissue lysates,Lane 3: mouse NIH3T3 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha 1 microglobulin antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Alpha 1 microglobulin at approximately 34KD. The expected band size for Alpha 1 microglobulin is at 39KD.)
Alpha 1 microglobulin, Polyclonal Antibody (Cat# AAA124687)
Full Name
Anti-Alpha 1 microglobulin Picoband antibody
Gene Names
AMBP; A1M; HCP; ITI; UTI; EDC1; HI30; ITIL; IATIL; ITILC
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot
Purity
Immunogen Affinity Purified
Pricing
IHC (Immunohistochemistry)
(Figure 4. IHC analysis of Bag1 using anti-Bag1 antibody (AAA124688).Bag1 was detected in paraffin-embedded section of rat testis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Bag1 Antibody (AAA124688) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Bag1, Polyclonal Antibody (Cat# AAA124688)
Full Name
Anti-Bag1 Picoband Antibody
Gene Names
BAG1; HAP; BAG-1; RAP46
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins
No cross reactivity with other proteins
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified
Pricing
IHC (Immunohistochemistry)
(Figure 5. IHC analysis of HSPB8/Hsp22 using anti- HSPB8/Hsp22 antibody (AAA124689).HSPB8/Hsp22 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- HSPB8/Hsp22 Antibody (AAA124689) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
HSPB8/Hsp22, Polyclonal Antibody (Cat# AAA124689)
Full Name
Anti-HSPB8/Hsp22 Picoband Antibody
Gene Names
HSPB8; H11; HMN2; CMT2L; DHMN2; E2IG1; HMN2A; HSP22
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins
No cross reactivity with other proteins
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified
Pricing
IHC (Immunohiostchemistry)
(Figure 2. IHC analysis of NAP2 using anti-NAP2 antibody (AAA124694).NAP2 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-NAP2 Antibody (AAA124694) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
NAP2, Polyclonal Antibody (Cat# AAA124694)
Full Name
Anti-NAP2 Picoband antibody
Gene Names
Ppbp; TGB; LDGF; MDGF; TGB1; CTAP3; Cxcl7; NAP-2; Scyb7; b-TG1; LA-PF4; THBGB1; CTAPIII; beta-TG; AI854500; NAP-2-L1; 2400003M24Rik
Reactivity
Mouse
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot, Immunohistochemistry
Pricing
IHC (Immunohistochemisry)
(Figure 3. IHC analysis of Caspase 4 using anti-Caspase 4 antibody (AAA124698).Caspase 4 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Caspase 4 Antibody (AAA124698) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Caspase 4, Polyclonal Antibody (Cat# AAA124698)
Full Name
Anti-Caspase 4 Picoband antibody
Gene Names
CASP4; TX; Mih1; ICH-2; Mih1/TX; ICEREL-II; ICE(rel)II
Reactivity
Human
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot, Immunohistochemistry
Pricing
IHC (Immunohistochemisry)
(Figure 3. IHC analysis of AKR1B10 using anti- AKR1B10 antibody (AAA124700).AKR1B10 was detected in paraffin-embedded section of human liver cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- AKR1B10 Antibody (AAA124700) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
AKR1B10, Polyclonal Antibody (Cat# AAA124700)
Full Name
Anti-AKR1B10 Picoband Antibody
Gene Names
AKR1B10; HIS; HSI; ARL1; ARL-1; ALDRLn; AKR1B11; AKR1B12
Reactivity
Human
No cross reactivity with other proteins
No cross reactivity with other proteins
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of SFTPB using anti-SFTPB antibody (AAA124707).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human MCF-7 whole cell lysates,Lane 2: human COLO-320 whole cell lysates,Lane 3: human HepG2 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFTPB antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SFTPB at approximately 46KD. The expected band size for SFTPB is at 42KD.)
SFTPB/Surfactant Protein B, Polyclonal Antibody (Cat# AAA124707)
Full Name
Anti-SFTPB/Surfactant Protein B Picoband antibody
Gene Names
SFTPB; SP-B; PSP-B; SFTB3; SFTP3; SMDP1
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Immunogen affinity purified
Pricing
What are Polyclonal Antibodies?
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. This ability to bind multiple epitopes is what makes polyclonal antibodies highly sensitive, as explained in our detailed guide on polyclonal antibodies and why they matter.
As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
- Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
- Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
- ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
- Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
- Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in our catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.