Filters
▼Clonality
▼Type
▼Reactivity
▼Gene Name
▼Isotype
▼Host
▼Application
▼Clone
▼Polyclonal Antibodies
At AAA Biotech also known as AAA Bio or AAABio, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
Viewing 3600-3650 of 10000 product results
IHC (Immunohistochemistry)
(AAA31102 at 1/100 staining Human prostate tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
ATP12A, Polyclonal Antibody (Cat# AAA31102)
Full Name
ATP12A Antibody
Gene Names
ATP12A; HK; ATP1AL1
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Pricing
WB (Western Blot)
(Western blot analysis of extracts from JK cells using US28 antibody.)
US28, Polyclonal Antibody (Cat# AAA31120)
Full Name
US28 Antibody
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
Peptide affinity purification
Pricing
IF (Immunofluorescence)
(AAA31124 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31124) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI (blue).)
PROS1, Polyclonal Antibody (Cat# AAA31124)
Full Name
PROS1 Antibody
Gene Names
PROS1; PSA; PROS; PS21; PS22; PS23; PS24; PS25; THPH5; THPH6
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Pricing
WB (Western Blot)
(Western blot analysis of extracts from various samples, using TIMP4 Antibody. Lane 1: B16F10, treated with blocking peptide; Lane 2: B16F10; Lane 3: Myeloma cells.)
TIMP4, Polyclonal Antibody (Cat# AAA31145)
Full Name
TIMP4 Antibody
Gene Names
TIMP4; TIMP-4
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
WB (Western Blot)
(Western blot analysis of BMP2 expression in HepG2 (A); HeLa (B) whole cell lysates.)
BMP2, Polyclonal Antibody (Cat# AAA12380)
Full Name
Anti-BMP2 Antibody (C-Terminus) IHC-plus
Gene Names
BMP2; BDA2; BMP2A
Reactivity
Chicken, Zebrafish, Pig, Rabbit, Mouse, Sheep, Bovine, Rat, Human
Applications
Immunohistochemistry, Western Blot
Purity
Immunoaffinity purified
Pricing
Application Data
H5 (A/Hong Kong/483/97)(H5N1), Polyclonal Antibody (Cat# AAA13731)
Full Name
Anti-H5 (A/Hong Kong/483/97)(H5N1), rabbit IgG
Reactivity
Has not been tested.
Applications
Western Blot
Purity
Immunoaffinity chromatography
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of A549 cells using anti-MDR3 antibody (AAA11652).Overlay histogram showing A549 cells stained with AAA11652 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MDR3 Antibody (AAA11652,1ug/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
ABCB4, Polyclonal Antibody (Cat# AAA11652)
Full Name
Anti-ABCB4 Antibody
Gene Names
ABCB4; GBD1; ICP3; MDR2; MDR3; PGY3; ABC21; MDR2/3; PFIC-3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
Immunogen Affinity Purified
Pricing
CIAS1/NALP3, Polyclonal Antibody (Cat# AAA11523)
Full Name
Anti-CIAS1/NALP3 antibody
Gene Names
NLRP3; AII; AVP; FCU; MWS; FCAS; CIAS1; NALP3; C1orf7; CLR1.1; PYPAF1; AGTAVPRL
Reactivity
Human, Rat; Predicted: Mouse
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry
Purity
Immunogen affinity purified
Pricing
WB (Western Blot)
(Western BlotPositive WB detected in Recombinant proteinAll lanes: speB antibody at 3.2ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionpredicted band size: 44 kDaobserved band size: 44 kDa)
speB, Polyclonal Antibody (Cat# AAA26985)
Full Name
speB Antibody
Reactivity
Streptococcus pyogenes
Applications
Western Blot
Purity
>95%, Protein G purified
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse kidney using SNRNP70 antibody (AAA28160) at dilution of 1:100 (40x lens).)
SNRNP70, Polyclonal Antibody (Cat# AAA28160)
Full Name
SNRNP70 Polyclonal Antibody
Gene Names
SNRNP70; RPU1; Snp1; U1AP; U170K; U1RNP; RNPU1Z; SNRP70; U1-70K
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purification
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Mouse stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
BRK, Polyclonal Antibody (Cat# AAA31371)
Full Name
Phospho-BRK (Tyr447) Antibody
Gene Names
PTK6; BRK
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Horse (89%)
Predicted Reactivity: Horse (89%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA31274 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31274 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
TTC7A, Polyclonal Antibody (Cat# AAA31274)
Full Name
TTC7A Antibody
Gene Names
TTC7A; TTC7; GIDID; MINAT
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(88%), Bovine(88%), Horse(88%), Sheep(88%), Rabbit(100%), Dog(86%)
Predicted Reactivity: Pig(88%), Bovine(88%), Horse(88%), Sheep(88%), Rabbit(100%), Dog(86%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
WB (Western Blot)
(Western blot analysis of Phospho-Connexin 43 (Ser367) expression in various lysates)
Connexin 43, Polyclonal Antibody (Cat# AAA31018)
Full Name
Phospho-Connexin 43 (Ser367) Antibody
Gene Names
GJA1; HSS; CMDR; CX43; EKVP; GJAL; ODDD; AVSD3; EKVP3; HLHS1; PPKCA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA31068 staining NIH/3T3 cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)
BCL-XL, Polyclonal Antibody (Cat# AAA31068)
Full Name
Phospho-BCL-XL (Thr47) Antibody
Gene Names
BCL2L1; BCLX; BCL2L; Bcl-X; PPP1R52; BCL-XL/S
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
eNOS, Polyclonal Antibody (Cat# AAA31394)
Full Name
Phospho-eNOS (Ser1179) Antibody
Gene Names
NOS3; eNOS; ECNOS
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Rabbit (100%), Dog (100%)
Predicted Reactivity: Pig (100%), Bovine (100%), Rabbit (100%), Dog (100%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(AAA31100 at 1/100 staining Rat lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the Ab for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit Ab was used as the secondary.)
DSS1, Polyclonal Antibody (Cat# AAA31100)
Full Name
DSS1 Antibody
Gene Names
SEM1; ECD; DSS1; SHFD1; SHFM1; SHSF1; Shfdg1; C7orf76
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
IF (Immunofluorescence)
(AAA31210 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31210) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
GLS2, Polyclonal Antibody (Cat# AAA31210)
Full Name
GLS2 Antibody
Gene Names
GLS2; GA; GLS; LGA; hLGA
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(86%), Horse(100%), Sheep(100%), Dog(88%)
Predicted Reactivity: Pig(86%), Horse(100%), Sheep(100%), Dog(88%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using PMS1 antibody (AAA28270) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)
PMS1, Polyclonal Antibody (Cat# AAA28270)
Full Name
PMS1 Polyclonal Antibody
Gene Names
PMS1; MLH2; PMSL1; hPMS1; HNPCC3
Applications
Western Blot
Purity
Affinity Purification
Pricing
IHC (Immunohistochemistry)
(The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using AAA29763 (ISM1 Antibody) at dilution 1/60.(Original magnification: 200))
ISM1, Polyclonal Antibody (Cat# AAA29763)
Full Name
ISM1 Antibody
Gene Names
ISM1; ISM; Isthmin; C20orf82; bA149I18.1; dJ1077I2.1
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Antigen affinity purification
Pricing
WB (Western Blot)
(lane C, 293 cell extract controlLane 1, 293 cell expressing HA2 (H5N1)Lane 2, 293 cell expressing HA (H5N1))
HA2 (A/Vietnam/1203/2004)(H5N1), Polyclonal Antibody (Cat# AAA13718)
Full Name
Anti-HA2 (A/Vietnam/1203/2004)(H5N1), rabbit IgG
Applications
Western Blot
Purity
Immunoaffinity chromatography
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of HepG2 cells using anti-ATF2 antibody (AAA11634).Overlay histogram showing HepG2 cells stained with AAA11634 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATF2 Antibody (AAA11634,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight ®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Cyclic AMP-dependent transcription factor ATF-2, Polyclonal Antibody (Cat# AAA11634)
Full Name
Anti-ATF2 Antibody
Gene Names
ATF2; HB16; CREB2; TREB7; CREB-2; CRE-BP1
Reactivity
Human, Mouse, Rat. No cross reactivity with other proteins.
Applications
Western Blot, Immunohistochemistry, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Western blot analysis of Akt1 phosphorylation expression in B2C whole cell lysates, The lane on the right is treated with the antigen-specific peptide.)
Akt1, Polyclonal Antibody (Cat# AAA31034)
Full Name
Phospho-Akt1 (Thr450) Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(AAA31069 at 1/50 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
FKHR, Polyclonal Antibody (Cat# AAA31069)
Full Name
Phospho-FKHR (Ser319) Antibody
Gene Names
FOXO1; FKH1; FKHR; FOXO1A
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA31077 staining HepG2 by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31077 1:200) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
ACE2, Polyclonal Antibody (Cat# AAA31077)
Full Name
ACE2 Antibody
Gene Names
ACE2; ACEH
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Peptide affinity purification
Pricing
IF (Immunofluorescence)
(AAA31241 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31241 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Sulfatase 1, Polyclonal Antibody (Cat# AAA31241)
Full Name
Sulfatase 1 Antibody
Gene Names
SULF1; SULF-1
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%), Xenopus(100%)
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%), Xenopus(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistochemistry-Paraffin)
(AAA31268 at 1/100 staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
PRMT4, Polyclonal Antibody (Cat# AAA31268)
Full Name
PRMT4 Antibody
Gene Names
CARM1; PRMT4
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Zebrafish(88%), Bovine(88%), Horse(100%), Sheep(88%), Dog(100%), Xenopus(100%)
Predicted Reactivity: Pig(100%), Zebrafish(88%), Bovine(88%), Horse(100%), Sheep(88%), Dog(100%), Xenopus(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(AAA31271 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31271 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Sts1, Polyclonal Antibody (Cat# AAA31271)
Full Name
Sts1 Antibody
Gene Names
UBASH3B; p70; STS1; STS-1; TULA2; TULA-2
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistochemistry)
(AAA31112 at 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-Rabbit Ab was used as the secondary Ab.)
OSR2, Polyclonal Antibody (Cat# AAA31112)
Full Name
OSR2 Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
WB (Western Blot)
(Western blot analysis of extracts from Hela, using Phospho-TLR4(Ser800) Antibody.)
TLR4, Polyclonal Antibody (Cat# AAA31162)
Full Name
Phospho-TLR4 (Ser800) Antibody
Gene Names
TLR4; TOLL; CD284; TLR-4; ARMD10
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA31168 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31168 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
DLL4, Polyclonal Antibody (Cat# AAA31168)
Full Name
DLL4 Antibody
Gene Names
DLL4; AOS6; delta4; hdelta2
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(88%)
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(88%)
Applications
Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(AAA31227 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31227 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
SET, Polyclonal Antibody (Cat# AAA31227)
Full Name
SET Antibody
Gene Names
SET; 2PP2A; IGAAD; MRD58; TAF-I; I2PP2A; IPP2A2; PHAPII; TAF-IBETA
Reactivity
Human, Rat
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(AAA31232 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31232 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
SMARCA1, Polyclonal Antibody (Cat# AAA31232)
Full Name
SMARCA1 Antibody
Gene Names
SMARCA1; SWI; ISWI; SWI2; SNF2L; SNF2L1; SNF2LB; SNF2LT; hSNF2L; NURF140
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Predicted Reactivity: Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
WB (Western Blot)
(Western blot analysis of eIF2 alpha phosphorylation expression in IFN-alpha treated K562 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
eIF2 alpha, Polyclonal Antibody (Cat# AAA30978)
Full Name
Phospho-eIF2 alpha (Ser51) Antibody
Gene Names
EIF2S1; EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(AAA31004 at 1/200 staining Rat brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Tau, Polyclonal Antibody (Cat# AAA31004)
Full Name
Phospho-Tau (Thr205) Antibody
Gene Names
MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(IHC(P):Rat Spleen Tissue)
FOXP3, Polyclonal Antibody (Cat# AAA11651)
Full Name
Anti-FOXP3 Antibody
Gene Names
FOXP3; JM2; AIID; IPEX; PIDX; XPID; DIETER
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry
Purity
Immunogen Affinity Purified
Pricing
Thrombocyte, Polyclonal Antibody (Cat# AAA14283)
Full Name
Anti-Mouse Thrombocyte, Antiserum, (Polyclonal) (rabbit serum)
Reactivity
Mouse
Applications
Agglutination Assay, Invivo Depletion
Pricing
IHC (Immunohistchemistry)
(DAB staining on IHC-P; Samples: Mouse Ovary Tissue))
Glycogen Synthase Kinase 3 Alpha (GSK3a), Polyclonal Antibody (Cat# AAA20310)
Full Name
Polyclonal Antibody to Glycogen Synthase Kinase 3 Alpha (GSK3a)
Gene Names
Gsk3a; 2700086H06Rik
Reactivity
Mouse, Human, Rat
Applications
Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Affinity Chromatography
Pricing
IHC (Immunohistochemistry)
(Anti-SLC7A5 antibody IHC of human placenta, terminal villi. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
SLC7A5 / CD98, Polyclonal Antibody (Cat# AAA12328)
Full Name
SLC7A5 / CD98 Light Chain
Gene Names
SLC7A5; E16; CD98; LAT1; 4F2LC; MPE16; hLAT1; D16S469E
Reactivity
Human, Monkey, Dog.
Predicted: Bat, Rabbit
Predicted: Bat, Rabbit
Applications
Immunohistochemistry
Purity
Immunoaffinity Purified
Pricing
QC (Quality Control)
(This Antibody has been quality control tested by immunohistochemistry as follows)
Mycobacterium tuberculosis, Polyclonal Antibody (Cat# AAA13576)
Full Name
Mycobacterium tuberculosis
Reactivity
Human
Applications
Immunohistochemistry
Pricing
WB (Western Blot)
(Anti-Cre Ab at 2000 dilution; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution.)
Cre, Polyclonal Antibody (Cat# AAA13864)
Full Name
Cre Recombinase Polyclonal Antibody
Reactivity
Bacteriophage P1
Applications
Western Blot
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of 293T cells using anti-ARG2 antibody (AAA19256).Overlay histogram showing 293T cells stained with AAA19256 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ARG2 Antibody (AAA19256, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
ARG2, Polyclonal Antibody (Cat# AAA19256)
Full Name
Anti-ARG2 Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA
Purity
Immunogen affinity purified.
Pricing
Knockout Validation
(Knockout Validation: Lane 1: Wild-type Hela cell lysate; Lane 2: POFUT1 knockout Hela cell lysate; Predicted MW: 44,22kd Observed MW: 44kd Primary Ab: 1ug/ml Rabbit Anti-Human POFUT1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody ( Human.)
Protein O-Fucosyltransferase 1, Polyclonal Antibody (Cat# AAA21025)
Full Name
Protein O-Fucosyltransferase 1 (POFUT1) Polyclonal Antibody
Gene Names
POFUT1; DDD2; FUT12; O-FUT; OFUCT1; O-Fuc-T; O-FucT-1
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry
Purity
Affinity Chromatography
Pricing
WB (Western Blot)
(WB Suggested Anti-ACTN1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: 293T cell lysate)
ACTN1, Polyclonal Antibody (Cat# AAA23579)
Full Name
ACTN1 antibody - N-terminal region
Gene Names
ACTN1; BDPLT15
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Applications
Immunohistochemistry, Western Blot
Purity
Affinity Purified
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of DNA Polymerase zeta staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
DNA Polymerase zeta, Polyclonal Antibody (Cat# AAA27811)
Full Name
Anti-DNA Polymerase zeta Antibody
Gene Names
REV3L; POLZ; REV3
Reactivity
Human, Mouse, Rat, Bovine, Chicken, Dog, Pig
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
RAD50, Polyclonal Antibody (Cat# AAA31432)
Full Name
Phospho-RAD50 (Ser635) Antibody
Gene Names
RAD50; NBSLD; RAD502; hRad50
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(AAA31023 at 1/200 staining Rat spleen tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
p70 S6 Kinase, Polyclonal Antibody (Cat# AAA31023)
Full Name
Phospho-p70 S6 Kinase (Thr229) Antibody
Gene Names
RPS6KB1; S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of Phospho-IL-8R beta/CDw128 beta (Ser347) expression in Mouse kidney lysate)
IL-8R beta/CDw128 beta, Polyclonal Antibody (Cat# AAA31026)
Full Name
Phospho-IL-8R beta/CDw128 beta (Ser347) Antibody
Gene Names
CXCR2; CD182; IL8R2; IL8RA; IL8RB; CMKAR2; CDw128b
Reactivity
Human, Mouse, Monkey
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of Phospho-SYK (Tyr525) expression in various lysates)
SYK, Polyclonal Antibody (Cat# AAA31052)
Full Name
Phospho-SYK (Tyr525) Antibody
Gene Names
SYK; p72-Syk
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of FAK phosphorylation expression in EGF treated 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
FAK, Polyclonal Antibody (Cat# AAA31067)
Full Name
Phospho-FAK (Tyr397) Antibody
Gene Names
PTK2; FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA31280 staining A549 cells(H2O2 treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(#AAA31280) and mouse anti-beta tubulin Ab(#) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI (blue).)
PYCARD, Polyclonal Antibody (Cat# AAA31280)
Full Name
Phospho-PYCARD (Ser195) Antibody
Gene Names
PYCARD; ASC; TMS; TMS1; CARD5; TMS-1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
What are Polyclonal Antibodies?
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
- Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
- Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
- ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
- Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
- Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.