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▼Clone
▼Polyclonal Antibodies
At AAA Biotech also known as AAA Bio or AAABio, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
Viewing 3500-3550 of 10000 product results
IF (Immunofluorescence)
(AAA31178 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31178) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
CENPB, Polyclonal Antibody (Cat# AAA31178)
Full Name
CENPB Antibody
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%)
Predicted Reactivity: Pig(100%), Bovine(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(AAA31225 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31225) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
KCNK9, Polyclonal Antibody (Cat# AAA31225)
Full Name
KCNK9 Antibody
Gene Names
KCNK9; KT3.2; TASK3; K2p9.1; TASK-3
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Chicken(83%), Xenopus(83%)
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Chicken(83%), Xenopus(83%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
WB (Western Blot)
(Western blot analysis on mouse liver tissue lysate using E-cadherin Antibody)
E-cadherin, Polyclonal Antibody (Cat# AAA30922)
Full Name
E-cadherin Antibody
Gene Names
CDH1; UVO; CDHE; ECAD; LCAM; Arc-1; BCDS1; CD324
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
WB (Western Blot)
(Anti-Venus Ab at 1/2,000 dilution; 293HEK cells transduced with Venus-Rab7 Ad; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;)
Venus, Polyclonal Antibody (Cat# AAA13879)
Full Name
Venus Polyclonal Antibody
Reactivity
Transfected cells proteins
Applications
Western Blot, Immunofluorescence, Immunohistochemistry, Immunohistochemistry
Pricing
WB (Western Blot)
(Western Blot analysis of Hela cells with JUN Polyclonal Antibody)
AP-1, Polyclonal Antibody (Cat# AAA22055)
Full Name
JUN Polyclonal Antibody
Gene Names
AP-1gamma; Ap-1; AP-1; AP-1 gamma; AP-1[gamma]; AP1gamma; AP1gamma1; apl4; cg9113; CG9113; dgamma; DmelCG9113; gamma; gamma-Ada
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purification
Pricing
WB (Western Blot)
(Positive WB detected in Recombinant proteinAll lanes: BZLF1 antibody at 1:1000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 46 kDaObserved band size: 50 kDa)
BZLF1, Polyclonal Antibody (Cat# AAA27059)
Full Name
Rabbit anti-Epstein-Barr virus (strain B95-8)(HHV-4)(Human herpesvirus 4) BZLF1 Polyclonal Antibody
Reactivity
Epstein-Barr virus
Applications
Western Blot
Purity
> 95%, Protein G purified
Pricing
IF (Immunofluorescence)
(AAA31193 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31193 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
CHD4, Polyclonal Antibody (Cat# AAA31193)
Full Name
CHD4 Antibody
Gene Names
CHD4; CHD-4; Mi-2b; SIHIWES; Mi2-BETA
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Predicted Reactivity: Pig(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Applications
Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(AAA31215 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31215 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
PDE11A, Polyclonal Antibody (Cat# AAA31215)
Full Name
PDE11A Antibody
Gene Names
PDE11A; PPNAD2
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Zebrafish(86%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Predicted Reactivity: Pig(100%), Zebrafish(86%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistchemistry-Paraffin)
(AAA31218 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Siglec 7, Polyclonal Antibody (Cat# AAA31218)
Full Name
Siglec 7 Antibody
Gene Names
SIGLEC7; p75; QA79; AIRM1; CD328; CDw328; D-siglec; SIGLEC-7; SIGLECP2; SIGLEC19P; p75/AIRM1
Reactivity
Human, Mouse, Rat
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistochemistry-Paraffin)
(AAA31236 at 1/100 staining Human gastric cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
OVOL2, Polyclonal Antibody (Cat# AAA31236)
Full Name
OVOL2 Antibody
Gene Names
OVOL2; CHED; CHED1; CHED2; PPCD1; ZNF339; EUROIMAGE566589
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
FCM (Flow Cytometry)
(Figure 14. Flow Cytometry analysis of SiHa cells using anti-PTRF/CAVIN1 antibody (AAA20011).Overlay histogram showing SiHa cells stained with AAA20011 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PTRF/CAVIN1 Antibody (AAA20011, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
PTRF/CAVIN1, Polyclonal Antibody (Cat# AAA20011)
Full Name
Anti-PTRF/CAVIN1 Antibody Picoband
Gene Names
PTRF; CGL4; CAVIN; CAVIN1; FKSG13; cavin-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry in formalin-fixed and paraffin-embedded human hepatocarcinoma using AAA24067, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of AAA24067 for immunohistochemistry.)
CD163, Polyclonal Antibody (Cat# AAA24067)
Full Name
CD163, NT (CD163, M130, Scavenger receptor cysteine-rich type 1 protein M130, Hemoglobin scavenger receptor, CD163, Soluble CD163)
Gene Names
CD163; M130; MM130
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Purified by ammonium sulfate precipitation.
Pricing
HBsAg, Polyclonal Antibody (Cat# AAA14317)
Full Name
HBsAg antibody
Reactivity
Specific for all 4 subtypes of Hepatitis B surface Antigens
Purity
HBsAg antibody was purified by affinity chromatography.
Pricing
IHC (Immunohistochemistry)
DWORF, Polyclonal Antibody (Cat# AAA14407)
Full Name
DWORF Antibody
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Affinity Purified Immunoglobulins
Pricing
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded human liver tissue using AAA28677 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:1000) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)
ATG7, Polyclonal Antibody (Cat# AAA28677)
Full Name
ATG7 Antibody (C-term)
Gene Names
ATG7; GSA7; APG7L; APG7-LIKE
Reactivity
Human, Mouse
Predicted reactivity: Chicken, Rat
Predicted reactivity: Chicken, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistochemistry-Paraffin)
(AAA31258 at 1/100 staining Rat liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
L3MBTL1, Polyclonal Antibody (Cat# AAA31258)
Full Name
L3MBTL1 Antibody
Gene Names
L3MBTL1; L3MBTL; ZC2HC3; H-L(3)MBT; dJ138B7.3
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Predicted Reactivity: Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistochemistry)
(AAA31051 at 1/200 staining Human bladder cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
SYK, Polyclonal Antibody (Cat# AAA31051)
Full Name
Phospho-SYK (Tyr348) Antibody
Gene Names
SYK; p72-Syk
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of extracts from HeLa cells, using Cytochrome P450 39A1 Antibody. The lane on the left was treated with blocking peptide.)
Cytochrome P450 39A1, Polyclonal Antibody (Cat# AAA31117)
Full Name
Cytochrome P450 39A1 Antibody
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
IHC (Immunohistochemistry)
(AAA31121 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
FABP4, Polyclonal Antibody (Cat# AAA31121)
Full Name
FABP4 Antibody
Gene Names
FABP4; aP2; ALBP; AFABP; A-FABP; HEL-S-104
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
IF (Immunofluorescence)
(AAA31177 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31177 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Proteasome 20S LMP7, Polyclonal Antibody (Cat# AAA31177)
Full Name
Proteasome 20S LMP7 Antibody
Gene Names
PSMB8; JMP; ALDD; LMP7; NKJO; D6S216; PRAAS1; PSMB5i; RING10; D6S216E
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Horse(86%), Rabbit(100%), Dog(88%)
Predicted Reactivity: Horse(86%), Rabbit(100%), Dog(88%)
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistochemistry-Paraffin)
(AAA31179 at 1/100 staining human breast carcinoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
CD36, Polyclonal Antibody (Cat# AAA31179)
Full Name
CD36 Antibody
Gene Names
CD36; FAT; GP4; GP3B; GPIV; CHDS7; PASIV; SCARB3; BDPLT10
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Peptide affinity purification
Pricing
IF (Immunofluorescence)
(AAA31230 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31230 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
ACTR1A, Polyclonal Antibody (Cat# AAA31230)
Full Name
ACTR1A Antibody
Gene Names
ACTR1A; ARP1; Arp1A; CTRN1
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
WB (Western Blot)
(HHLA2 Antibody (N-term) western blot analysis in MDA-MB453,CEM,HL-60,MDA-MB231 cell line lysates (35ug/lane).This demonstrates the HHLA2 antibody detected the HHLA2 protein (arrow).)
HHLA2, Polyclonal Antibody (Cat# AAA28722)
Full Name
HHLA2 Antibody (N-term)
Gene Names
HHLA2; B7H7; B7-H7
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
Elafin, Polyclonal Antibody (Cat# AAA27749)
Full Name
Anti-Elafin Antibody, Rabbit Polyclonal
Gene Names
PI3; ESI; WAP3; SKALP; WFDC14; cementoin
Applications
ELISA
Purity
Protein A affinity chromatography
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U-2 OS cells using PP2A-B56δ/PR61δ/PP2A-B56δ/PR61δ/PPP2R5D antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
PP2A-B56delta/PR61delta/PPP2R5D, Polyclonal Antibody (Cat# AAA28319)
Full Name
PP2A-B56delta/PR61delta/PPP2R5D Rabbit pAb
Gene Names
PPP2R5D; B56D
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(AAA31246 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31246) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
TROY, Polyclonal Antibody (Cat# AAA31246)
Full Name
TROY Antibody
Gene Names
TNFRSF19; TAJ; TROY; TRADE; TAJ-alpha
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Horse(88%), Sheep(86%)
Predicted Reactivity: Horse(88%), Sheep(86%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistochemistry-Paraffin)
(AAA31254 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Hhip, Polyclonal Antibody (Cat# AAA31254)
Full Name
Hhip Antibody
Gene Names
HHIP; HIP
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunocytochemistry, Western Blot, Immunohistochemistry
Purity
Peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
IHC (Immunohistchemistry)
(At 1/100 staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Ephrin B1/B2/B3, Polyclonal Antibody (Cat# AAA31292)
Full Name
Phospho-Ephrin B1/B2/B3 (Tyr324) Antibody
Gene Names
EFNB1; CFND; CFNS; EFL3; EPLG2; Elk-L; LERK2
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Peptide ELISA
Purity
Peptide affinity purification
Pricing
Application Data
(AAA31020 staining HepG2 cells(30min of 4uM Forskolin treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31020) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI(blue))
VEGFR1, Polyclonal Antibody (Cat# AAA31020)
Full Name
Phospho-VEGFR1 (Tyr1213) Antibody
Gene Names
FLT1; FLT; FLT-1; VEGFR1; VEGFR-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, Immunohistochemistry
Purity
The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(AAA31106 at 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
GPR141, Polyclonal Antibody (Cat# AAA31106)
Full Name
GPR141 Antibody
Gene Names
GPR141; PGR13
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(AAA31213 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31213 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Exportin-5, Polyclonal Antibody (Cat# AAA31213)
Full Name
Exportin-5 Antibody
Gene Names
XPO5; exp5
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(88%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(88%), Dog(100%), Chicken(100%)
Predicted Reactivity: Pig(88%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(88%), Dog(100%), Chicken(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistochemistry)
(Figure 7. IHC analysis of Flotillin 2 using anti-Flotillin 2 antibody (AAA11664).Flotillin 2 was detected in frozen section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Flotillin 2 Antibody (AAA11664) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Flotillin 2, Polyclonal Antibody (Cat# AAA11664)
Full Name
Anti-Flotillin 2 Antibody
Gene Names
FLOT2; ESA; ECS1; ESA1; ECS-1; M17S1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen Affinity Purified
Pricing
IHC (Immunohistochemistry)
(Anti-GFAP antibody IHC of human brain, cortex, astrocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
GFAP, Polyclonal Antibody (Cat# AAA12316)
Full Name
Goat Polyclonal to Human GFAP
Reactivity
Human, Monkey, Rat, Bat, Bovine, Horse, Rabbit
Applications
Immunohistochemistry, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitacion followed by antigen affinity chromatography using the immunizing peptide.
Pricing
IF (Immunofluorescence)
(AAA13648 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).)
AKAP9/AKAP450/CG-NAP, Polyclonal Antibody (Cat# AAA13648)
Full Name
Goat anti-AKAP9/AKAP450/CG-NAP Antibody
Gene Names
AKAP9; LQT11; PRKA9; AKAP-9; CG-NAP; YOTIAO; AKAP350; AKAP450; PPP1R45; HYPERION; MU-RMS-40.16A
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse
Applications
Peptide ELISA, Western Blot, Immunofluorescence
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry: Lung tissue (FFPE) stained with Rabbit anti-ER81 (Cat# AAA14073) at 1:200 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)
ER81, Polyclonal Antibody (Cat# AAA14073)
Full Name
Rabbit anti ER81 Polyclonal Antibody
Gene Names
ETV1; ER81
Reactivity
Mouse, Human, Rat
Applications
Flow Cytometry, Immunohistochemistry, Western Blot, Immunoprecipitation
Purity
Rabbit IgG is purified by Epitope Affinity Purification
Pricing
WB (Western Blot)
(Western blot analysis of 30 ug of whole cell lysate (A: A549) using a 12 % SDS PAGE gel and NKG2D antibody at a dilution of 1:1000)
NKG2D, Polyclonal Antibody (Cat# AAA17934)
Full Name
NKG2D antibody
Applications
Immunohistochemistry, Western Blot
Purity
NKG2D antibody was purified by antigen-affinity chromatography
Pricing
IHC (Immunohistochemistry)
(DAB staining on fromalin fixed paraffin- embedded liver tissue))
Cyclin Dependent Kinase Inhibitor 2A (CDKN2A), Polyclonal Antibody (Cat# AAA20299)
Full Name
Polyclonal Antibody to Cyclin Dependent Kinase Inhibitor 2A (CDKN2A)
Gene Names
CDKN2A; ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A
Reactivity
Human
Applications
Westen Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
WB (Western Blot)
(TGFBR2 Antibody western blot of mouse lung tissue lysates (35 ug/lane). The TGFBR2 antibody detected the TGFBR2 protein (arrow).)
TGFBR2, Polyclonal Antibody (Cat# AAA21269)
Full Name
Anti-TGFBR2 Antibody (aa13-40) IHC-plus
Gene Names
TGFBR2; AAT3; FAA3; LDS2; MFS2; RIIC; LDS1B; LDS2B; TAAD2; TGFR-2; TGFbeta-RII
Reactivity
Mouse, Human
Applications
Immunohistochemistry, Immunofluorescence, Western Blot
Purity
Protein A purified
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of HepG2 cells using AAA26915 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Beta-defensin 1, Polyclonal Antibody (Cat# AAA26915)
Full Name
Rabbit anti-Homo sapiens (Human) DEFB1 Polyclonal antibody
Gene Names
DEFB1; BD1; HBD1; DEFB-1; DEFB101
Reactivity
Human
Applications
Immunohistochemistry, Immunofluorescence
Purity
>95%, Protein G purified
Pricing
WB (Western Blot)
(OMP Antibody (N-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the OMP antibody detected the OMP protein (arrow).)
OMP, Polyclonal Antibody (Cat# AAA28786)
Full Name
OMP Antibody (N-term)
Reactivity
Human
Applications
Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Histone H2B, Polyclonal Antibody (Cat# AAA31352)
Full Name
Acetyl-Histone H2B (Lys20) Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
IHC (Immunohistchemistry)
(AAA31038 at 1/200 staining Rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IRS-1, Polyclonal Antibody (Cat# AAA31038)
Full Name
Phospho-IRS-1 (Ser639) Antibody
Gene Names
IRS1; HIRS-1
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of p53 phosphorylation expression in Etoposide treated 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
p53, Polyclonal Antibody (Cat# AAA30976)
Full Name
Phospho-p53 (Ser392) Antibody
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC-P (Immunohistochemistry-Paraffin)
(At 1/200 staining Rat ganstric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediatedantigen retrieval step in citrate buffer was performed. Thetissue was then blocked and incubated with the antibody for1.5 hours at 22°C. An HRP conjugated goat anti-rabbitantibody was used as the secondary antibody.)
c-Kit, Polyclonal Antibody (Cat# AAA31007)
Full Name
Phospho-c-Kit (Tyr721) Antibody
Gene Names
KIT; PBT; SCFR; C-Kit; CD117; MASTC
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Peptide affinity purification
Pricing
IHC (Immunohistochemistry-Paraffin)
(AAA31245 at 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Nesprin 1, Polyclonal Antibody (Cat# AAA31245)
Full Name
Nesprin 1 Antibody
Gene Names
SYNE1; 8B; AMCM; CPG2; ARCA1; EDMD4; KASH1; MYNE1; Nesp1; SCAR8; C6orf98; dJ45H2.2
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Horse(88%), Sheep(100%), Dog(88%)
Predicted Reactivity: Horse(88%), Sheep(100%), Dog(88%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(AAA31272 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31272 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
DERL2, Polyclonal Antibody (Cat# AAA31272)
Full Name
DERL2 Antibody
Gene Names
DERL2; FLANa; F-LANa; CGI-101; F-LAN-1; derlin-2; DERtrin-2
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunocytochemistry, Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Pricing
WB (Western Blot)
((2ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
CLEC16A, Polyclonal Antibody (Cat# AAA13667)
Full Name
Goat anti-CLEC16A (aa1040-53) Antibody
Gene Names
CLEC16A; Gop-1; KIAA0350
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
Peptide ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of Rat lung tissue using Nrf2 Polyclonal Antibody at dilution of 1:200.)
Nrf2, Polyclonal Antibody (Cat# AAA22058)
Full Name
Nrf2 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
Application Data
(Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.)
FGF21, Polyclonal Antibody (Cat# AAA23563)
Full Name
FGF21 antibody - N-terminal region
Reactivity
Tested Species Reactivity: Human
Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
Applications
Immunohistochemistry, Western Blot
Purity
Affinity Purified
Pricing
IHC (Immunohistochemistry)
(Immunofluorescent analysis of HepG2 cells using AAA26964 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
CTH, Polyclonal Antibody (Cat# AAA26964)
Full Name
CTH Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
>95%, Protein G purified
Pricing
What are Polyclonal Antibodies?
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
- Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
- Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
- ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
- Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
- Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.