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▼Polyclonal Antibodies
At AAA Biotech also known as AAA Bio or AAABio, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
Viewing 4000-4050 of 118597 product results
IF (Immunofluorescence)
(Immunofluorescent analysis of CD172a staining in THP1 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
CD172a, Polyclonal Antibody (Cat# AAA220942)
Full Name
Rabbit anti-CD172a Antibody
Gene Names
SIRPA; BIT; MFR; P84; SIRP; MYD-1; SHPS1; CD172A; PTPNS1
Reactivity
Human, Monkey
Applications
Immunoprecipitation, Immunochemistry, Immunofluorescence, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of Galectin 8 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Galectin 8, Polyclonal Antibody (Cat# AAA220950)
Full Name
Rabbit anti-Galectin 8 Antibody
Gene Names
LGALS8; Gal-8; PCTA1; PCTA-1; Po66-CBP
Reactivity
Human, Mouse
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of Smoothened staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Smoothened, Polyclonal Antibody (Cat# AAA220484)
Full Name
Rabbit anti-Smoothened Antibody
Gene Names
SMO; Gx; SMOH; FZD11
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of Histone H1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Histone H1, Polyclonal Antibody (Cat# AAA220713)
Full Name
Rabbit anti-Histone H1 Antibody
Gene Names
HIST1H1A; H1A; H1.1; H1F1; HIST1
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of Histone H3 (DiMethyl K14) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Histone H3, Polyclonal Antibody (Cat# AAA220714)
Full Name
Rabbit anti-Histone H3 (DiMethyl K14) Antibody
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunochemical staining of human CDC26 in human prostate with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
CDC26, Polyclonal Antibody (Cat# AAA258970)
Full Name
Anti-CDC26 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunohistochemistry
Purity
Protein A & Antigen Affinity
Pricing
IF (Immunofluorescence)
(Immunofluorescence staining of CGGBP1 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human CGGBP1 polyclonal antibody (dilution ratio 1:100) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Nucleus.)
CGGBP1, Polyclonal Antibody (Cat# AAA258971)
Full Name
Anti-CGGBP1 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
IP (Immunoprecipitation)
(DENR was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg 293T Whole Cell LysateLane C:0.5 mg Jurkat Whole Cell Lysate4 uL anti-DENR rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-DENR rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 22 kDaObserved band size :28 kDa)
DENR, Polyclonal Antibody (Cat# AAA259018)
Full Name
Anti-DENR Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
IP (Immunoprecipitation)
(MRPL28 was immunoprecipitated using:Lane A:0.5 mg A549 Whole Cell Lysate4 uL anti-MRPL28 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-MRPL28 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 30 kDaObserved band size :30 kDa)
MRPL28, Polyclonal Antibody (Cat# AAA259034)
Full Name
Anti-MRPL28 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
IF (Immunofluorescence)
(Immunofluorescence staining of PHGDH in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human PHGDH polyclonal antibody (dilution ratio 1:100) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
PHGDH, Polyclonal Antibody (Cat# AAA259039)
Full Name
Anti-PHGDH Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Pricing
IP (Immunoprecipitation)
(GAP43 was immunoprecipitated using:Lane A:0.5 mg SH-SY5Y Whole Cell Lysate4 uL anti-GAP43 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-GAP43 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 25 kDaObserved band size :45 kDa)
GAP-43, Polyclonal Antibody (Cat# AAA259043)
Full Name
Anti-GAP-43 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
IP (Immunoprecipitation)
(IGF2BP1 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate4 uL anti-IGF2BP1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-IGF2BP1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 64 kDaObserved band size :72 kDa)
IGF2BP1, Polyclonal Antibody (Cat# AAA259054)
Full Name
Anti-IGF2BP1 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
IP (Immunoprecipitation)
(AKAP8 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate4 uL anti-AKAP8 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-AKAP8 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 76 kDaObserved band size :95 kDa)
AKAP8, Polyclonal Antibody (Cat# AAA259058)
Full Name
Anti-AKAP8 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of KLHL29 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
KLHL29, Polyclonal Antibody (Cat# AAA259791)
Full Name
Anti-KLHL29 Antibody
Gene Names
KLHL29; KBTBD9
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of RB1 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
RB1, Polyclonal Antibody (Cat# AAA259794)
Full Name
Anti-RB1 Antibody
Gene Names
RB1; RB; pRb; OSRC; pp110; p105-Rb; PPP1R130
Reactivity
Human, Mouse, Rat, Monkey
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of PE2 staining in human ovarian cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
PE2, Polyclonal Antibody (Cat# AAA259810)
Full Name
Anti-PE2 Antibody
Gene Names
ERF; PE2; CRS4; PE-2
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of S6K1 (pS434) staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
NF-kappaB p65, Polyclonal Antibody (Cat# AAA259812)
Full Name
Anti-NF-kappaB p65 (AcK310) Acetyl Antibody
Gene Names
RELA; p65; NFKB3
Reactivity
Human, Mouse, Rat, Bovine, Porcine
Applications
Chromatin immunoprecipitation, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of DNAL4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
DNAL4, Polyclonal Antibody (Cat# AAA259822)
Full Name
Anti-DNAL4 Antibody
Gene Names
DNAL4; MRMV3; PIG27
Reactivity
Human, Mouse, Rat, Bovine, Porcine
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of CRK staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
CRK, Polyclonal Antibody (Cat# AAA259825)
Full Name
Anti-CRK Antibody
Gene Names
CRK; p38; CRKII
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of GCLM staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
GCLM, Polyclonal Antibody (Cat# AAA259831)
Full Name
Anti-GCLM Antibody
Gene Names
GCLM; GLCLR
Reactivity
Human, Mouse, Rat, Bovine
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of NGEP staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
NGEP, Polyclonal Antibody (Cat# AAA259624)
Full Name
Anti-NGEP Antibody
Gene Names
ANO7; NGEP; DTMPP; IPCA5; D-TMPP; IPCA-5; PCANAP5; TMEM16G; PCANAP5L
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of Histone H3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Histone H3, Polyclonal Antibody (Cat# AAA259664)
Full Name
Anti-Histone H3 Antibody
Gene Names
H3F3A; H3F3; H3.3A
Reactivity
Human, Mouse, Rat, Bovine, Chicken, Porcine, Zebrafish
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of Vinculin staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Vinculin, Polyclonal Antibody (Cat# AAA259670)
Full Name
Anti-Vinculin Antibody
Gene Names
VCL; MV; MVCL; CMD1W; CMH15; HEL114
Reactivity
Human, Mouse, Rat, Chicken, Porcine
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of CHSY2 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
CHSY2, Polyclonal Antibody (Cat# AAA259675)
Full Name
Anti-CHSY2 Antibody
Gene Names
CHPF; CSS2; CHSY2
Reactivity
Human, Monkey
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
WB (Western Blot)
(Western blot analysis of RPL22 expression in PC12 (A), MCF7 (B) whole cell lysates.)
RPL22, Polyclonal Antibody (Cat# AAA259688)
Full Name
Anti-RPL22 Antibody
Reactivity
Human, Mouse, Rat, Bovine, Chicken, Porcine
Applications
Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IP (Immunoprecipitation)
(TPP2 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate4 uL anti-TPP2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-TPP2 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 138 kDaObserved band size :138 kDa)
TPP2, Polyclonal Antibody (Cat# AAA259059)
Full Name
Anti-TPP2 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
IP (Immunoprecipitation)
(SUFU was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate4 uL anti-SUFU rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-SUFU rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 54 kDaObserved band size :54 kDa)
SUFU, Polyclonal Antibody (Cat# AAA259068)
Full Name
Anti-SUFU Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
IP (Immunoprecipitation)
(RTN4IP1 was immunoprecipitated using:Lane A:0.5 mg Mouse heart Whole Cell Lysate4 uL anti-RTN4IP1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-RTN4IP1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 44 kDaObserved band size :42 kDa)
RTN4IP1, Polyclonal Antibody (Cat# AAA259072)
Full Name
Anti-RTN4IP1 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
IHC (Immunohistochemisry)
(Immunochemical staining of human RAD9B in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
RAD9B, Polyclonal Antibody (Cat# AAA259075)
Full Name
Anti-RAD9B Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
WB (Western Blot)
(Anti-C17orf97 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:23 kDaObserved band size:45 kDa)
C17orf97, Polyclonal Antibody (Cat# AAA259089)
Full Name
Anti-C17orf97 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunoprecipitation, Immunohistochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
IP (Immunoprecipitation)
(NTMT1 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg MCF-7 Whole Cell Lysate4 uL anti-NTMT1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-NTMT1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 25 kDaObserved band size :25 kDa)
METTL11A, Polyclonal Antibody (Cat# AAA259097)
Full Name
Anti-METTL11A Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
IP (Immunoprecipitation)
(CASP7 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate4 uL anti-CASP7 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-CASP7 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 38 kDaObserved band size :38 kDa)
Caspase-7, Polyclonal Antibody (Cat# AAA259107)
Full Name
Anti-Caspase-7 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
IHC (Immunohiostchemistry)
(Immunochemical staining of human HUWE1 in human testis with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
HUWE1, Polyclonal Antibody (Cat# AAA259110)
Full Name
Anti-HUWE1 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
IP (Immunoprecipitation)
(mCD274 was immunoprecipitated using:Lane A:0.5 mg Mouse Liver Whole Cell Lysate4 uL anti-mCD274 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-mCD274 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 33 kDaObserved band size :50 kDa)
PD-L1/B7-H1, Polyclonal Antibody (Cat# AAA259123)
Full Name
Anti-PD-L1/B7-H1 Antibody, Rabbit Polyclonal
Reactivity
Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
IP (Immunoprecipitation)
(NEGR1 was immunoprecipitated using:Lane A:0.5 mg Mouse Brain Whole Cell Lysate4 uL anti-NEGR1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-NEGR1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 38 kDaObserved band size :40 kDa)
NEGR1, Polyclonal Antibody (Cat# AAA259133)
Full Name
Anti-NEGR1 Antibody, Rabbit Polyclonal
Reactivity
Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of CLOCK staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
CLOCK, Polyclonal Antibody (Cat# AAA259702)
Full Name
Anti-CLOCK Antibody
Gene Names
CLOCK; KAT13D; bHLHe8
Reactivity
Human, Mouse, Rat, Chicken, Dog, Porcine
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of Estrogen Receptor beta staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Estrogen Receptor beta, Polyclonal Antibody (Cat# AAA259707)
Full Name
Anti-Estrogen Receptor beta Antibody
Gene Names
ESR2; Erb; ESRB; ESTRB; NR3A2; ER-BETA; ESR-BETA
Reactivity
Human, Mouse, Rat, Dog
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
WB (Western Blot)
(Western blot analysis of FOXP3 expression in HEK293T (A), Raw264.7 (B), PC12 (C) whole cell lysates.)
FOXP3, Polyclonal Antibody (Cat# AAA259732)
Full Name
Anti-FOXP3 Antibody
Gene Names
FOXP3; JM2; AIID; IPEX; PIDX; XPID; DIETER
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of ZNF174 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
ZNF174, Polyclonal Antibody (Cat# AAA259754)
Full Name
Anti-ZNF174 Antibody
Gene Names
ZNF174; ZSCAN8
Reactivity
Human, Mouse, Rat, Monkey
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
WB (Western Blot)
(Western blot analysis of TGFBR3 expression in HEK293T (A), Raw264.7 (B), PC12 (C) whole cell lysates.)
TGFBR3, Polyclonal Antibody (Cat# AAA259775)
Full Name
Anti-TGFBR3 Antibody
Gene Names
TGFBR3; BGCAN; betaglycan
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of ZNF148 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
ZNF148, Polyclonal Antibody (Cat# AAA260338)
Full Name
Anti-ZNF148 Antibody
Gene Names
ZNF148; BERF-1; BFCOL1; ZBP-89; ZFP148; pHZ-52; HT-BETA
Reactivity
Human, Bovine
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of IGFBP5 staining in mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
IGFBP5, Polyclonal Antibody (Cat# AAA262636)
Full Name
Anti-IGFBP5 Antibody
Gene Names
IGFBP5; IBP5
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of GBL staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
GBL, Polyclonal Antibody (Cat# AAA262675)
Full Name
Anti-GBL Antibody
Gene Names
MLST8; GBL; LST8; POP3; WAT1; GbetaL
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of CRY1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
CRY1, Polyclonal Antibody (Cat# AAA262559)
Full Name
Anti-CRY1 Antibody
Gene Names
CRY1; PHLL1
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of BAP1 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
BAP1, Polyclonal Antibody (Cat# AAA262510)
Full Name
Anti-BAP1 Antibody
Gene Names
BAP1; UCHL2; hucep-6; HUCEP-13
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of BMI1 staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
BMI1, Polyclonal Antibody (Cat# AAA262516)
Full Name
Anti-BMI1 Antibody
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of MRP8 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
MRP8, Polyclonal Antibody (Cat# AAA262769)
Full Name
Anti-MRP8 Antibody
Gene Names
S100A8; P8; MIF; NIF; CAGA; CFAG; CGLA; L1Ag; MRP8; CP-10; MA387; 60B8AG
Reactivity
Human, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of TIA1 staining in SKOV3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
TIA1, Polyclonal Antibody (Cat# AAA262807)
Full Name
Anti-TIA1 Antibody
Gene Names
TIA1; WDM; TIA-1
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of TM9SF1 staining in human prostate formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
TM9SF1, Polyclonal Antibody (Cat# AAA262812)
Full Name
Anti-TM9SF1 Antibody
Gene Names
TM9SF1; MP70; HMP70
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of GILZ staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
GILZ, Polyclonal Antibody (Cat# AAA262821)
Full Name
Anti-GILZ Antibody
Gene Names
TSC22D3; DIP; GILZ; DSIPI; TSC-22R
Reactivity
Human, Mouse
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
What are Polyclonal Antibodies?
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. This ability to bind multiple epitopes is what makes polyclonal antibodies highly sensitive, as explained in our detailed guide on polyclonal antibodies and why they matter.
As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
- Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
- Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
- ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
- Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
- Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in our catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.