IHC (Immunohiostchemistry)
(Immunochemical staining of human HUWE1 in human testis with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Rabbit anti-Human HUWE1 Polyclonal Antibody | anti-HUWE1 antibody
Anti-HUWE1 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
HUWE1, Antibody; Anti-HUWE1 Antibody, Rabbit Polyclonal; anti-HUWE1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human HUWE1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-HUWE1 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
E coli-derived Human HUWE1 fragment
Preparation
Produced in rabbits immunized with E coli-derived Human HUWE1 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
IHC (Immunohiostchemistry)
(Immunochemical staining of human HUWE1 in human testis with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human HUWE1 in human testis with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
WB (Western Blot)
(Anti-HUWE1 rabbit polyclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLane B: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:481 kDa)
WB (Western Blot)
(Anti-HUWE1 rabbit polyclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLane B: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:481 kDa)
Related Product Information for anti-HUWE1 antibody
HUWE1 (HECT, UBA And WWE Domain Containing E3 Ubiquitin Protein Ligase 1) is a large proteins. There are conflicting reports of the role of HUWE1 in regulating the oncogene c-MYC (MYC). In one model, HUWE1 regulates the stability of MYC through K48-linked ubiquitination and knockdown of HUWE1 leads to increased MYC and drives tumor progression [16]. Conversely, in other tumor types HUWE1 activates transcription of MYC through K63-linked ubiquitination and HUWE1 silencing or inhibition prevents MYC signaling and reduces tumor cell proliferation. HUWE1 may play a cell-type and context-dependent role in MYC regulation.
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Product Notes
The HUWE1 (Catalog #AAA259110) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-HUWE1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HUWE1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the HUWE1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HUWE1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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