IP (Immunoprecipitation)
(Detection of human 14-3-3-theta by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-14-3-3-theta antibody AAA212124 used for IP at 6 ug/mg lysate. 14-3-3-theta was also immunoprecipitated by rabbit anti-14-3-3-theta antibody which recognizes a downstream epitope. For blotting immunoprecipitated 14-3-3-theta, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human 14-3-3 theta Polyclonal Antibody | anti-14-3-3theta antibody
Rabbit anti-14-3-3 theta Antibody, Affinity Purified
Gene Names
YWHAQ; 1C5; HS1; 14-3-3
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation
Purity
Antigen Affinity Purified
Synonyms
14-3-3 theta, Antibody; Rabbit anti-14-3-3 theta Antibody, Affinity Purified; HS1; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide; Protein HS1; 1C5; 14-3-3 theta; 14-3-3 protein theta; 14-3-3 protein T-cell; 14-3-3 protein tau; 14-3-3; protein, theta; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta; anti-14-3-3theta antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-14-3-3theta antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation)
Predicted Reactivity
Bovine, Orangutan
Immunogen
Between 125 and 175
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
IP (Immunoprecipitation)
(Detection of human 14-3-3-theta by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-14-3-3-theta antibody AAA212124 used for IP at 6 ug/mg lysate. 14-3-3-theta was also immunoprecipitated by rabbit anti-14-3-3-theta antibody which recognizes a downstream epitope. For blotting immunoprecipitated 14-3-3-theta, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human 14-3-3-theta by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-14-3-3-theta antibody AAA212124 used for IP at 6 ug/mg lysate. 14-3-3-theta was also immunoprecipitated by rabbit anti-14-3-3-theta antibody which recognizes a downstream epitope. For blotting immunoprecipitated 14-3-3-theta, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemistry)
(Detection of human 14-3-3-theta by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-14-3-3-theta (Cat. No. AAA212124 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human 14-3-3-theta by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-14-3-3-theta (Cat. No. AAA212124 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-14-3-3theta antibody
14-3-3 theta is a member of the 14-3-3 family of proteins which serve as adapter proteins in specialized signal transduction pathways. There are 7 members of the 14-3-3 family of proteins: beta, gamma, epsilon, zeta, eta, theta, and sigma.
Product Categories/Family for anti-14-3-3theta antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27,764 Da
NCBI Official Full Name
14-3-3 protein theta
NCBI Official Synonym Full Names
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide
NCBI Official Symbol
YWHAQ
NCBI Official Synonym Symbols
1C5; HS1; 14-3-3
NCBI Protein Information
14-3-3 protein theta; 14-3-3 protein tau; 14-3-3 protein T-cell
UniProt Protein Name
14-3-3 protein theta
UniProt Gene Name
YWHAQ
UniProt Entry Name
1433T_HUMAN
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Product Notes
The 14-3-3theta ywhaq (Catalog #AAA212124) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-14-3-3 theta Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's 14-3-3 theta can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the 14-3-3theta ywhaq for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "14-3-3 theta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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