WB (Western Blot)
(Anti-HPGD rabbit polyclonal antibody at 1:500 dilutionLane A: Lovo Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:29 kDaObserved band size:34 kDa(We are unsure as to the identity of these extra bands.))
Rabbit anti-Mouse 15-PGDH Polyclonal Antibody | anti-HPGD antibody
Anti-15-PGDH Antibody, Rabbit Polyclonal
Gene Names
Hpgd; 15-PGDH; AV026552
Reactivity
Mouse
Applications
Immunoprecipitation, ELISA, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
15-PGDH, Antibody; Anti-15-PGDH Antibody, Rabbit Polyclonal; HPGD/15-PGDH Antibody, Rabbit PAb, Antigen Affinity Purified; hydroxyprostaglandin dehydrogenase 15-(NAD); Anti-15-PGDH Antibody; Anti-AV026552 Antibody; anti-HPGD antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Mouse HPGD/15-PGDH
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-HPGD antibody
IP (Immunoprecipitation), ELISA, WB (Western Blot)
Immunogen
Recombinant Mouse HPGD/15-PGDH protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Mouse HPGD/15-PGDH (rM HPGD/15-PGDH). HPGD/15-PGDH specific IgG was purified by Mouse HPGD/15-PGDH affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
WB (Western Blot)
(Anti-HPGD rabbit polyclonal antibody at 1:500 dilutionLane A: Lovo Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:29 kDaObserved band size:34 kDa(We are unsure as to the identity of these extra bands.))
WB (Western Blot)
(Anti-HPGD rabbit polyclonal antibody at 1:500 dilutionLane A: Lovo Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:29 kDaObserved band size:34 kDa(We are unsure as to the identity of these extra bands.))
IP (Immunoprecipitation)
(Mouse HPGD was immunoprecipitated using:Lane A:0.5 mg Caco-2 Whole Cell Lysate2 uL anti-Mouse HPGD rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse HPGD rabbit polyclonal antibody,at 1:200 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 29 kDaObserved band size: 29 kDa)
IP (Immunoprecipitation)
(Mouse HPGD was immunoprecipitated using:Lane A:0.5 mg Caco-2 Whole Cell Lysate2 uL anti-Mouse HPGD rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse HPGD rabbit polyclonal antibody,at 1:200 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 29 kDaObserved band size: 29 kDa)
Related Product Information for anti-HPGD antibody
15-hydroxyprostaglandin dehydrogenase [NAD+], also known as Prostaglandin dehydrogenase 1, HPGD, and PGDH1, is a member of the short-chain dehydrogenases/reductases (SDR) family. Prostaglandins (PGs) play a key role in the onset of labor in many species and regulate uterine contractility and cervical dilatation. Therefore, the regulation of prostaglandin output by PG synthesizing and metabolizing enzymes in the human myometrium may determine uterine activity patterns in human labor both at preterm and at term. Prostaglandin dehydrogenase (PGDH) metabolizes prostaglandins (PGs) to render them inactive. HPGD is down-regulated by cortisol, dexamethasone, and betamethasone and down-regulated in colon cancer. It is up-regulated by TGFB1. HPGD contributes to the regulation of events that are under the control of prostaglandin levels. HPGD catalyzes the NAD-dependent dehydrogenation of lipoxin A4 to form 15-oxo-lipoxin A4. and inhibits in vivo proliferation of colon cancer cells. Defects in HPGD are the cause of primary hypertrophic osteoarthropathy autosomal recessive (PHOAR), cranio-osteoarthropathy (COA), and isolated congenital nail clubbing.
References
Patel, FA. et al., 2003, J. Clin. Endocrinol. Metab. 88: 2922-33. McKeown KJ, et al., 2003, J. Clin. Endocrinol. Metab. 88 (4): 1737-41. Yan, M. et al., 2004, Proc. Natl. Acad. Sci. USA. 101: 17468-73. Tariq, M. et al., 2009, J Med Genet. 46 (1): 14-20.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
29,181 Da
NCBI Official Full Name
15-hydroxyprostaglandin dehydrogenase
NCBI Official Synonym Full Names
hydroxyprostaglandin dehydrogenase 15 (NAD)
NCBI Official Symbol
Hpgd
NCBI Official Synonym Symbols
15-PGDH; AV026552
NCBI Protein Information
15-hydroxyprostaglandin dehydrogenase [NAD(+)]
UniProt Protein Name
15-hydroxyprostaglandin dehydrogenase [NAD(+)]
UniProt Gene Name
Hpgd
UniProt Synonym Gene Names
Pgdh1; 15-PGDH
Similar Products
Product Notes
The HPGD hpgd (Catalog #AAA257175) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-15-PGDH Antibody, Rabbit Polyclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's 15-PGDH can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the HPGD hpgd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "15-PGDH, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.