IF (Immunofluorescence)
(Confocal immunofluorescent analysis of 27Kip1 Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
Rabbit 27Kip1 Polyclonal Antibody | anti-CDKN1B antibody
27Kip1 Antibody (C-term)
Gene Names
CDKN1B; KIP1; MEN4; CDKN4; MEN1B; P27KIP1
Reactivity
Human (Predicted Reactivity: Hamster)
Applications
Immunofluorescence, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
27Kip1, Antibody; 27Kip1 Antibody (C-term); Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; p27Kip1; CDKN1B; KIP1; anti-CDKN1B antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Hamster)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This 27Kip1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 165-194 amino acids from the C-terminal region of human 27Kip1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.42 (varies by lot)
Sequence Positions
165-194
Sequence Length
198
Applicable Applications for anti-CDKN1B antibody
IF (Immunofluorescence), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of 27Kip1 Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of 27Kip1 Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
WB (Western Blot)
(Western blot analysis of p27Kip1 Antibody (C-term) in HepG2 cell line lysates (35ug/lane). 27Kip1(arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of p27Kip1 Antibody (C-term) in HepG2 cell line lysates (35ug/lane). 27Kip1(arrow) was detected using the purified Pab.)
Related Product Information for anti-CDKN1B antibody
p27Kip1 is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.
Product Categories/Family for anti-CDKN1B antibody
References
Kawamata, N., et al., Eur. J. Haematol. 74(5):424-429 (2005).
Andreu, E.J., et al., Cancer Res. 65(8):3264-3272 (2005).
Wingate, H., et al., J. Biol. Chem. 280(15):15148-15157 (2005).
Wang, C., et al., J. Biol. Chem. 280(13):12339-12343 (2005).
Rassidakis, G.Z., et al., Blood 105(2):827-829 (2005).
Andreu, E.J., et al., Cancer Res. 65(8):3264-3272 (2005).
Wingate, H., et al., J. Biol. Chem. 280(15):15148-15157 (2005).
Wang, C., et al., J. Biol. Chem. 280(13):12339-12343 (2005).
Rassidakis, G.Z., et al., Blood 105(2):827-829 (2005).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22073
NCBI Official Full Name
cyclin-dependent kinase inhibitor 1B
NCBI Official Synonym Full Names
cyclin-dependent kinase inhibitor 1B (p27, Kip1)
NCBI Official Symbol
CDKN1B
NCBI Official Synonym Symbols
KIP1; MEN4; CDKN4; MEN1B; P27KIP1
NCBI Protein Information
cyclin-dependent kinase inhibitor 1B
UniProt Protein Name
Cyclin-dependent kinase inhibitor 1B
UniProt Gene Name
CDKN1B
UniProt Synonym Gene Names
KIP1
UniProt Entry Name
CDN1B_HUMAN
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Product Notes
The CDKN1B cdkn1b (Catalog #AAA289837) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 165-194. The 27Kip1 Antibody (C-term) reacts with Human (Predicted Reactivity: Hamster) and may cross-react with other species as described in the data sheet. AAA Biotech's 27Kip1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CDKN1B cdkn1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "27Kip1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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