Rabbit anti-Human 3-phosphoinositide-dependent protein kinase 1 Polyclonal Antibody | anti-PDPK1 antibody

Rabbit anti-human 3-phosphoinositide-dependent protein kinase 1 polyclonal Antibody(PDPK1)

Cat. Num. AAA119067

• Gene Names: PDPK1; PDK1; PDPK2; PDPK2P; PRO0461
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Antigen Affinity Purified
• Synonyms: 3-phosphoinositide-dependent protein kinase 1, Antibody; Rabbit anti-human 3-phosphoinositide-dependent protein kinase 1 polyclonal Antibody(PDPK1); 3-phosphoinositide-dependent protein kinase 1; hPDK1; PDPK1; PDK1; anti-PDPK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Sequence Length: 454

• Applicable Applications for anti-PDPK1 antibody: IHC (Immunohistochemistry), WB (Western Blot), ELISA
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Conjugate: Non-conjugated
• Immunogen: Recombinant human 3-phosphoinositide-dependent protein kinase 1 protein(277-556aa)
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-7686 / sc-9118
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

IHC (Immunohiostchemistry)

(Immunohistochemistry of paraffin-embedded human thyroid using AAA119067 at dilution 1:100)

WB (Western Blot)

(All lanes: 3-phosphoinositide-dependent protein kinase 1 antibody at 2.5ug/mlLane 1:Hela whole cell lysateLane 2:A549 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 64,59,61,49,51 kDaObserved band size: 63 kDa)

Related Product Information for anti-PDPK1 antibody

Serine/threonine kinase which acts as a master kinase, phosphorylating and activating a subgroup of the AGC family of protein kinases. Its targets include: protein kinase B (PKB/AKT1, PKB/AKT2, PKB/AKT3), p70 ribosomal protein S6 kinase (RPS6KB1), p90 ribosomal protein S6 kinase (RPS6KA1, RPS6KA2 and RPS6KA3), cyclic AMP-dependent protein kinase (PRKACA), protein kinase C (PRKCD and PRKCZ), serum and glucocorticoid-inducible kinase (SGK1, SGK2 and SGK3), p21-activated kinase-1 (PAK1), protein kinase PKN (PKN1 and PKN2). Plays a central role in the transduction of signals from insulin by providing the activating phosphorylation to PKB/AKT1, thus propagating the signal to downstream targets controlling cell proliferation and survival, as well as glucose and amino acid uptake and storage. Negatively regulates the TGF-beta-induced signaling by: modulating the association of SMAD3 and SMAD7 with TGF-beta receptor, phosphorylating SMAD2, SMAD3, SMAD4 and SMAD7, preventing the nuclear translocation of SMAD3 and SMAD4 and the translocation of SMAD7 from the nucleus to the cytoplasm in response to TGF-beta. Activates PPARG transcriptional activity and promotes adipocyte differentiation. Activates the NF-kappa-B pathway via phosphorylation of IKKB. The tyrosine phosphorylated form is crucial for the regulation of focal adhesions by angiotensin II. Controls proliferation, survival, and growth of developing pancreatic cells. Participates in the regulation of Ca2+ entry and Ca2+-activated K+ channels of mast cells. Essential for the motility of vascular endothelial cells (ECs) and is involved in the regulation of their chemotaxis. Plays a critical role in cardiac homeostasis by serving as a dual effector for cell survival and beta-adrenergic response. Plays an important role during thymocyte development by regulating the expression of key nutrient receptors on the surface of pre-T cells and mediating Notch-induced cell growth and proliferative responses. Provides negative feedback inhibition to toll-like receptor-mediated NF-kappa-B activation in macrophages. Isoform 3 is catalytically inactive.

NCBI and Uniprot Product Information

• NCBI GI #: 387849239
• NCBI GeneID: 5170
• NCBI Accession #: NP_001248745.1
• NCBI GenBank Nucleotide #: NM_001261816.1
• UniProt Accession #: O15530; Q59EH6; Q6FI20; Q8IV52; Q9BRD5; H0Y4Z0; Q6A1A2
• Molecular Weight: 50,838 Da
• NCBI Official Full Name: 3-phosphoinositide-dependent protein kinase 1 isoform 3
• NCBI Official Synonym Full Names: 3-phosphoinositide dependent protein kinase 1
• NCBI Official Symbol: PDPK1
• NCBI Official Synonym Symbols: PDK1; PDPK2; PDPK2P; PRO0461
• NCBI Protein Information: 3-phosphoinositide-dependent protein kinase 1
• UniProt Protein Name: 3-phosphoinositide-dependent protein kinase 1
• UniProt Gene Name: PDPK1
• UniProt Synonym Gene Names: PDK1; hPDK1
• UniProt Entry Name: PDPK1_HUMAN