IHC (Immunohiostchemistry)
(Dilution: WB: (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)Immunohistochemical analysis of PDPK1 (pS241) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Rabbit PDPK1 Polyclonal Antibody | anti-PDPK1 antibody
Anti-PDPK1 (pS241) Antibody
Gene Names
PDPK1; PDK1; PDPK2; PRO0461
Reactivity
Human, Mouse, Rat, Rabbit, Pig, Bovine, SARS
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Synonyms
PDPK1, Antibody; Anti-PDPK1 (pS241) Antibody; PDK1; 3-phosphoinositide-dependent protein kinase 1; hPDK1; anti-PDPK1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Rabbit, Pig, Bovine, SARS
Clonality
Polyclonal
Applicable Applications for anti-PDPK1 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(Dilution: WB: (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)Immunohistochemical analysis of PDPK1 (pS241) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
IHC (Immunohiostchemistry)
(Dilution: WB: (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)Immunohistochemical analysis of PDPK1 (pS241) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
WB (Western Blot)
(Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)Western blot analysis of PDPK1 (pS241) expression in mouse liver (A), mouse lung (B), rat liver (C) whole cell lysates.)
WB (Western Blot)
(Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)Western blot analysis of PDPK1 (pS241) expression in mouse liver (A), mouse lung (B), rat liver (C) whole cell lysates.)
Related Product Information for anti-PDPK1 antibody
Rabbit polyclonal antibody to PDPK1 (pS241)
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PDPK1. The exact sequence is proprietary.
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PDPK1. The exact sequence is proprietary.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
63,152 Da
NCBI Official Full Name
3-phosphoinositide-dependent protein kinase 1 isoform 3
NCBI Official Synonym Full Names
3-phosphoinositide dependent protein kinase-1
NCBI Official Symbol
PDPK1
NCBI Official Synonym Symbols
PDK1; PDPK2; PRO0461
NCBI Protein Information
3-phosphoinositide-dependent protein kinase 1; PkB-like 1; PkB kinase like gene 1
UniProt Protein Name
3-phosphoinositide-dependent protein kinase 1
UniProt Gene Name
PDPK1
UniProt Synonym Gene Names
PDK1; hPDK1
UniProt Entry Name
PDPK1_HUMAN
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Product Notes
The PDPK1 pdpk1 (Catalog #AAA292335) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PDPK1 (pS241) Antibody reacts with Human, Mouse, Rat, Rabbit, Pig, Bovine, SARS and may cross-react with other species as described in the data sheet. AAA Biotech's PDPK1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PDPK1 pdpk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDPK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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