IP (Immunoprecipitation)
(mNT5E was immunoprecipitated using:Lane A:0.5 mg Mouse spleen Whole Cell Lysate4 uL anti-mNT5E rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-mNT5E rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 63 kDaObserved band size :70 kDa)
Rabbit anti-Mouse 5'-nucleotidase/CD73/NT5E Polyclonal Antibody | anti-CD73/NT5E antibody
Anti-5'-nucleotidase/CD73/NT5E Antibody, Rabbit Polyclonal
Reactivity
Mouse
Applications
Immunoprecipitation, Immunohistochemistry, ELISA, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
5'-nucleotidase/CD73/NT5E, Antibody; Anti-5'-nucleotidase/CD73/NT5E Antibody, Rabbit Polyclonal; 5'-nucleotidase; ecto (CD73); Anti-2210401F01Rik Antibody; Anti-AI447961 Antibody; Anti-CD73 Antibody; Anti-eNT Antibody; Anti-NT Antibody; Anti-Nt5 Antibody; anti-CD73/NT5E antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Mouse 5'-nucleotidase/CD73/NT5E
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-CD73/NT5E antibody
IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Immunogen
Recombinant Mouse CD73 Protein
Preparation
Produced in rabbits immunized with purified, recombinant Mouse CD73 (rM CD73). CD73 specific IgG was purified by Mouse CD73 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(mNT5E was immunoprecipitated using:Lane A:0.5 mg Mouse spleen Whole Cell Lysate4 uL anti-mNT5E rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-mNT5E rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 63 kDaObserved band size :70 kDa)
IP (Immunoprecipitation)
(mNT5E was immunoprecipitated using:Lane A:0.5 mg Mouse spleen Whole Cell Lysate4 uL anti-mNT5E rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-mNT5E rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 63 kDaObserved band size :70 kDa)
IHC (Immunohiostchemistry)
(Immunochemical staining of Mouse CD73 in Mouse kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of Mouse CD73 in Mouse kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
WB (Western Blot)
(Anti-CD73 rabbit polyclonal antibody at 1:500 dilutionLane A: Mouse spleen tissue lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:63 kDa)
WB (Western Blot)
(Anti-CD73 rabbit polyclonal antibody at 1:500 dilutionLane A: Mouse spleen tissue lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:63 kDa)
Related Product Information for anti-CD73/NT5E antibody
5'-nucleotidase, also known as NT5E, NTE, and CD73, is a cell membrane protein that belongs to the 5'-nucleotidase family. CD73 is a glycosylphosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes. CD73 catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides. CD73 serves as a costimulatory molecule in activating T cells. CD73 generated adenosine functions in cell signaling in many physiologic systems, including intestinal epithelium, ischemic myocardium, and cholinergic synapses. CD73 might mediate lymphocyte-stromal cell interactions or condition the local microenvironment to facilitate lymphocyte development and/or function. In CD73-depleted cells, surface levels of the leukocyte adhesion molecules ICAM-1, VCAM-1, and E-selectin increase. CD73 produces extracellular adenosine, which then acts on G protein-coupled purinergic receptors to induce cellular responses. CD73 has also been reported to regulate the expression of pro-inflammatory molecules in mouse endothelium.
References
Resta R. et al., 1997, Cell Signal. 9 (2): 131-9.
Yamashita Y. et al., 1998, Eur J Immunol. 28 (10): 2981-90.
Louis NA. et al., 2008, J Immunol. 180 (6): 4246-55.
Grünewald JK. et al., 2010, J Inflamm. 7 (1): 10.
Yamashita Y. et al., 1998, Eur J Immunol. 28 (10): 2981-90.
Louis NA. et al., 2008, J Immunol. 180 (6): 4246-55.
Grünewald JK. et al., 2010, J Inflamm. 7 (1): 10.
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Product Notes
The CD73/NT5E (Catalog #AAA259127) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-5'-nucleotidase/CD73/NT5E Antibody, Rabbit Polyclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's 5'-nucleotidase/CD73/NT5E can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CD73/NT5E for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "5'-nucleotidase/CD73/NT5E, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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