Standard Curve (Sample)
(Mouse NT5E PicoKine ELISA Kit standard curve)
Mouse CD73/NT5E ELISA Kit | NT5E elisa kit
Mouse CD73/NT5E ELISA Kit PicoKine
Gene Names
Nt5e; NT; Nt5; eNT; CD73; 5'-NT; AI447961; 2210401F01Rik
Reactivity
Mouse
Applications
ELISA
Synonyms
CD73/NT5E; N/A; Mouse CD73/NT5E ELISA Kit PicoKine; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; Tumor necrosis factor, membrane form; N-terminal fragment; NTF; Intracellular domain 1; ICD1; Intracellular domain 2; ICD2; C-domain 1; C-domain 2; Tumor necrosis factor, soluble form; Tnf; Tnfa, Tnfsf2; 5' nucleotidase, ecto; NT5E elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant Mouse Nt5e
Samples
Cell culture supernatants, serum and plasma (heparin, EDTA)
Detection Range
0.156 ng/ml - 10 ng/ml
Sensitivity
<20 pg/ml
Applicable Applications for NT5E elisa kit
ELISA
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Standard Curve (Sample)
(Mouse NT5E PicoKine ELISA Kit standard curve)
Standard Curve (Sample)
(Mouse NT5E PicoKine ELISA Kit standard curve)
Related Product Information for NT5E elisa kit
Background: 5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (Cluster of Differentiation 73), is an enzyme that in humans is encoded by the NT5E gene. Ecto-5-prime-nucleotidase catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. And the enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto- NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. Rareallelic variants are associated with a syndrome of adult-onset calcification of joints and arteries (CALJA) affecting the iliac, femoral, and tibial arteries reducing circulation in the legs and the joints of the hands and feet causing pain.
Principle of the Assay: The Picokine™ Mouse Nt5e Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Mouse Nt5e with a 96-well strip plate that is pre-coated with antibody specific for Nt5e. The detection antibody is a biotinylated antibody specific for Nt5e. The capture antibody is a monoclonal antibody from rat and the detection antibody is a biotinylated polyclonal antibody from goat. The kit includes Mouse Nt5e protein as standards. To measure Mouse Nt5e, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Mouse Nt5e in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Nt5e in the sample.
Principle of the Assay: The Picokine™ Mouse Nt5e Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Mouse Nt5e with a 96-well strip plate that is pre-coated with antibody specific for Nt5e. The detection antibody is a biotinylated antibody specific for Nt5e. The capture antibody is a monoclonal antibody from rat and the detection antibody is a biotinylated polyclonal antibody from goat. The kit includes Mouse Nt5e protein as standards. To measure Mouse Nt5e, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Mouse Nt5e in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Nt5e in the sample.
Product Categories/Family for NT5E elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
63,864 Da
NCBI Official Full Name
5'-nucleotidase
NCBI Official Synonym Full Names
5' nucleotidase, ecto
NCBI Official Symbol
Nt5e
NCBI Official Synonym Symbols
NT; Nt5; eNT; CD73; 5'-NT; AI447961; 2210401F01Rik
NCBI Protein Information
5'-nucleotidase
UniProt Protein Name
5'-nucleotidase
UniProt Gene Name
Nt5e
UniProt Synonym Gene Names
Nt5; Nte; 5'-NT
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Product Notes
The Mouse NT5E nt5e (Catalog #AAA126680) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA126680 ELISA Kit recognizes Mouse NT5E. AAA Biotech's CD73/NT5E can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the NT5E nt5e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD73/NT5E, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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