WB (Western Blot)
(Western blot analysis of different tissue and cell lysates using rabbit pAb to the serotonin transporter (SERT), AAA76743, dilution 1:1,000 in green. Left: [1] protein standard, [2] rat brain caudate/putmen region, [3] rat brain striatum region and [4] mouse brain. Right: [5] protein standard, [6] non-transfected HEK293 cells and [7] HEK293 cells transfected with an expression construct containing a Myc-DDK tagged full length human SERT cDNA.)
Rabbit 5HT (Serotonin) Transporter Polyclonal Antibody | anti-5HT antibody
5HT (Serotonin) Transporter
Gene Names
SLC6A4; HTT; 5HTT; OCD1; SERT; 5-HTT; SERT1; hSERT; 5-HTTLPR
Reactivity
Human, Mouse, Rat, Bovine
Applications
Immunofluorescence, Immunocytochemistry
Synonyms
5HT (Serotonin) Transporter, Antibody; 5HT (Serotonin) Transporter; anti-5HT antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine
Clonality
Polyclonal
Isotype
Rabbit IgG
Form/Format
Liquid. Affinity purified antibody at 1mg/mL in 50% PBS, 50%glycerol plus 5mM NaN3
Applicable Applications for anti-5HT antibody
IF (Immunofluorescence), ICC (Immunocytochemistry)
Immunogen
KSITPETPTEIPCGDIRLNAV coupled to KLH
Preparation and Storage
Antibody can also be aliquotted and stored frozen at -20 degree C in a manual defrost freezer for six months without detectable loss of activity. The antibody can be stored at 2 degree C - 8 degree C for 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of different tissue and cell lysates using rabbit pAb to the serotonin transporter (SERT), AAA76743, dilution 1:1,000 in green. Left: [1] protein standard, [2] rat brain caudate/putmen region, [3] rat brain striatum region and [4] mouse brain. Right: [5] protein standard, [6] non-transfected HEK293 cells and [7] HEK293 cells transfected with an expression construct containing a Myc-DDK tagged full length human SERT cDNA.)
WB (Western Blot)
(Western blot analysis of different tissue and cell lysates using rabbit pAb to the serotonin transporter (SERT), AAA76743, dilution 1:1,000 in green. Left: [1] protein standard, [2] rat brain caudate/putmen region, [3] rat brain striatum region and [4] mouse brain. Right: [5] protein standard, [6] non-transfected HEK293 cells and [7] HEK293 cells transfected with an expression construct containing a Myc-DDK tagged full length human SERT cDNA.)
IF (Immunofluorescence)
(Immunofluorescent analysis of a rat brain section stained with AAA76743 in green and counterstained with chicken polyclonal antibody to tyrosine hydroxylase in red. The axons of serotoninergic neurons course throughout the section but are clearly distinct from the chatacholinergic processes and cell bodies revealed with the TH antibody. The blue stain reveals DNA in cell nuclei.)
IF (Immunofluorescence)
(Immunofluorescent analysis of a rat brain section stained with AAA76743 in green and counterstained with chicken polyclonal antibody to tyrosine hydroxylase in red. The axons of serotoninergic neurons course throughout the section but are clearly distinct from the chatacholinergic processes and cell bodies revealed with the TH antibody. The blue stain reveals DNA in cell nuclei.)
Related Product Information for anti-5HT antibody
Serotonin, also known as 5-hydroxytryptamine, is an important neurotransmitter in the CNS and has many important functions outside the CNS. Serotoninergic neuron cell bodies in the CNS are found in the raphe nucleus and send their fine beaded processes throughout the brain and spinal cord. The serotoninergic system modulates mood, perception, emotion, aggression, stress responses, sleep and appetite and contributes to learning and memory formation. The processes of serotoninergic neurons express the Na+/Cl- dependent serotonin transporter (SERT), which is responsible for uptake of serotonin from the extracellular space following synaptic release.
It was made against a peptide identical to the C-terminal 21 amino acids of human SERT and can be used to visualize serotoninergic neurons and their processes in cell culture and in sectioned material. The homologous rodent peptide differs by one conservative amino acid substitution and we have shown that this antibody works well on rodent and bovine tissues and, based on sequence conservation, is expected to be widely applicable to other mammals.
It was made against a peptide identical to the C-terminal 21 amino acids of human SERT and can be used to visualize serotoninergic neurons and their processes in cell culture and in sectioned material. The homologous rodent peptide differs by one conservative amino acid substitution and we have shown that this antibody works well on rodent and bovine tissues and, based on sequence conservation, is expected to be widely applicable to other mammals.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
70,325 Da
NCBI Official Full Name
serotonin transporter, serotonin uptake site, 5-HT uptake site
NCBI Official Synonym Full Names
solute carrier family 6 (neurotransmitter transporter), member 4
NCBI Official Symbol
SLC6A4
NCBI Official Synonym Symbols
HTT; 5HTT; OCD1; SERT; 5-HTT; SERT1; hSERT; 5-HTTLPR
NCBI Protein Information
sodium-dependent serotonin transporter; 5HT transporter; serotonin transporter 1; 5-hydroxytryptamine transporter; Na+/Cl- dependent serotonin transporter; 5-hydroxytryptamine (serotonin) transporter; solute carrier family 6 (neurotransmitter transporter,
UniProt Protein Name
Sodium-dependent serotonin transporter
UniProt Gene Name
SLC6A4
UniProt Synonym Gene Names
HTT; SERT; 5HTT
UniProt Entry Name
SC6A4_HUMAN
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Product Notes
The 5HT slc6a4 (Catalog #AAA76743) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The 5HT (Serotonin) Transporter reacts with Human, Mouse, Rat, Bovine and may cross-react with other species as described in the data sheet. AAA Biotech's 5HT (Serotonin) Transporter can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry). Researchers should empirically determine the suitability of the 5HT slc6a4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "5HT (Serotonin) Transporter, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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