IHC (Immunohistochemistry)
(Abbreviated Immunohistochemical Protocol)
Rabbit A-1-Antichymotrypsin Polyclonal Antibody | anti-SERPINA3 antibody
A-1-Antichymotrypsin
Gene Names
SERPINA3; ACT; AACT; GIG24; GIG25; MGC88254
Reactivity
Human; Paraffin, Frozen
Synonyms
A-1-Antichymotrypsin, Antibody; A-1-Antichymotrypsin; anti-SERPINA3 antibody
Host
Rabbit
Reactivity
Human; Paraffin, Frozen
Clonality
Polyclonal
Isotype
IgG
Immunogen
Purified human a-1-antichymotrypsin.
Localization
Cytoplasmic
Control
Tonsil, Lymph Node
Presentation
Anti-A-1-Antichymotrypsin is a purified immunoglobulin fraction of rabbit antiserum that is filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Precautions
1. This product contains <0.1% sodium azide (NaN?) as a preservative. Ensure proper handling procedures are used with this reagent.
2. Always wear personal protective equipment such as a laboratory coat, goggles, and gloves when handling reagents.
3. Dispose of unused solution with copious amounts of water.
4. Do not ingest reagent. If reagent is ingested, seek medical advice immediately.
5. Avoid contact with eyes. If contact occurs, flush with large quantities of water.
6. Follow safety precautions of the heating device used for epitope retrieval (TintoRetriever Pressure Cooker or similar).
7. For additional safety information refer to Safety Data Sheet for this product.
8. For complete recommendations for handling biological specimens, please refer to the CDC document, “Guidelines for Safe Work Practices in Human and AMDL” (see References in this document).
2. Always wear personal protective equipment such as a laboratory coat, goggles, and gloves when handling reagents.
3. Dispose of unused solution with copious amounts of water.
4. Do not ingest reagent. If reagent is ingested, seek medical advice immediately.
5. Avoid contact with eyes. If contact occurs, flush with large quantities of water.
6. Follow safety precautions of the heating device used for epitope retrieval (TintoRetriever Pressure Cooker or similar).
7. For additional safety information refer to Safety Data Sheet for this product.
8. For complete recommendations for handling biological specimens, please refer to the CDC document, “Guidelines for Safe Work Practices in Human and AMDL” (see References in this document).
Product Limitations
Due to inherent variability present in immunohistochemical procedures (including fixation time of tissues, dilution factor of antibody, retrieval method utilized, and incubation time), optimal performance should be established through the use of positive and negative controls.
Mounting Protocols
For detailed instructions using biodegradable permanent mounting media such as XyGreen PermaMounter or organic solvent based resin such as PermaMounter.
Preparation and Storage
Store at 2 to 8 degree C in the dark.
IHC (Immunohistochemistry)
(Abbreviated Immunohistochemical Protocol)
IHC (Immunohistochemistry)
(Abbreviated Immunohistochemical Protocol)
IHC (Immunohistochemistry)
(IHC of Alpha-1-Antichymotrypsin on an FFPE Tonsil Tissue)
IHC (Immunohistochemistry)
(IHC of Alpha-1-Antichymotrypsin on an FFPE Tonsil Tissue)
Related Product Information for anti-SERPINA3 antibody
This antibody is intended for use in Immunohistochemical applications on formalin-fixed paraffin-embedded tissues (FFPE), frozen tissue sections and cell preparations.
Summary and Explanation: Alpha-1-Antichymotrypsin Glycoprotein found in alpha (1)-globulin region in human serum. It inhibits chymotrypsin-like proteinases in vivo and has cytotoxic killer-cell activity in vitro. The protein also has a role as an acute-phase protein and is active in the control of immunologic and inflammatory processes, and as a tumor marker. It is a member of the serpin superfamily.
Alpha-1-Antichymotrypsin antibody reacts with histiocytes and histiocytic neoplasms. Its major application is defining the presence of Alpha-1-Antichymotrypsin in histiocytes and tumors derived from them. In eosinophilic granuloma and malignant histiocytosis, the reaction for this marker is heterogeneous in intensity and distribution. In fibrous histiocytomas, under certain circumstances, a diffuse homogeneous reaction may be observed.
Summary and Explanation: Alpha-1-Antichymotrypsin Glycoprotein found in alpha (1)-globulin region in human serum. It inhibits chymotrypsin-like proteinases in vivo and has cytotoxic killer-cell activity in vitro. The protein also has a role as an acute-phase protein and is active in the control of immunologic and inflammatory processes, and as a tumor marker. It is a member of the serpin superfamily.
Alpha-1-Antichymotrypsin antibody reacts with histiocytes and histiocytic neoplasms. Its major application is defining the presence of Alpha-1-Antichymotrypsin in histiocytes and tumors derived from them. In eosinophilic granuloma and malignant histiocytosis, the reaction for this marker is heterogeneous in intensity and distribution. In fibrous histiocytomas, under certain circumstances, a diffuse homogeneous reaction may be observed.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
alpha-1-antichymotrypsin
NCBI Official Synonym Full Names
serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3
NCBI Official Symbol
SERPINA3
NCBI Official Synonym Symbols
ACT; AACT; GIG24; GIG25; MGC88254
NCBI Protein Information
alpha-1-antichymotrypsin; serpin A3; OTTHUMP00000028439; growth-inhibiting protein 24; growth-inhibiting protein 25; cell growth-inhibiting gene 24/25 protein; serine (or cysteine) proteinase inhibitor, clade A, member 3
UniProt Protein Name
Alpha-1-antichymotrypsin
UniProt Gene Name
SERPINA3
UniProt Synonym Gene Names
AACT
UniProt Entry Name
AACT_HUMAN
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Product Notes
The SERPINA3 serpina3 (Catalog #AAA59423) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The A-1-Antichymotrypsin reacts with Human; Paraffin, Frozen and may cross-react with other species as described in the data sheet. It is sometimes possible for the material contained within the vial of "A-1-Antichymotrypsin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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