IF (Immunofluorescence)
(Immunofluorescent analysis of A-MYB staining in SGC7901 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).)
Rabbit A-MYB Polyclonal Antibody | anti-MYBL1 antibody
Anti-A-MYB Antibody
Gene Names
MYBL1; AMYB; A-MYB
Reactivity
Human, Mouse, Rat, Bovine, Dog, Porcine
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
A-MYB, Antibody; Anti-A-MYB Antibody; AMYB; Myb-related protein A; A-Myb; Myb-like protein 1; anti-MYBL1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Dog, Porcine
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of A-MYB protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Sequence Length
752
Applicable Applications for anti-MYBL1 antibody
ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human A-MYB. The exact sequence is proprietary.
Preparation and Storage
Upon receipt aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
IF (Immunofluorescence)
(Immunofluorescent analysis of A-MYB staining in SGC7901 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).)
IF (Immunofluorescence)
(Immunofluorescent analysis of A-MYB staining in SGC7901 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).)
WB (Western Blot)
(Western blot analysis of A-MYB expression in mouse brain (A), rat brain (B) whole cell lysates.(Predicted band size: 85 kD; Observed band size: 90 kD))
WB (Western Blot)
(Western blot analysis of A-MYB expression in mouse brain (A), rat brain (B) whole cell lysates.(Predicted band size: 85 kD; Observed band size: 90 kD))
Related Product Information for anti-MYBL1 antibody
Rabbit polyclonal antibody to A-MYB
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
myb-related protein A isoform 1
NCBI Official Synonym Full Names
v-myb avian myeloblastosis viral oncogene homolog-like 1
NCBI Official Symbol
MYBL1
NCBI Official Synonym Symbols
AMYB; A-MYB
NCBI Protein Information
myb-related protein A
UniProt Protein Name
Myb-related protein A
UniProt Gene Name
MYBL1
UniProt Synonym Gene Names
AMYB; A-Myb
UniProt Entry Name
MYBA_HUMAN
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Product Notes
The MYBL1 mybl1 (Catalog #AAA259847) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-A-MYB Antibody reacts with Human, Mouse, Rat, Bovine, Dog, Porcine and may cross-react with other species as described in the data sheet. AAA Biotech's A-MYB can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the MYBL1 mybl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "A-MYB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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