FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of U87 cells using anti-v-Myb/MYBL1 antibody (AAA125759).Overlay histogram showing U87 cells stained with AAA125759 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-v-Myb/MYBL1 Antibody (AAA125759, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Rabbit v-Myb/MYBL1 Polyclonal Antibody | anti-MYBL1 antibody
Anti-v-Myb/MYBL1 Antibody
Gene Names
MYBL1; AMYB; A-MYB
Reactivity
Human, Mouse, Rat
Applications
ELISA, Flow Cytometry, Functional Assay, Western Blot
Purity
Immunogen affinity purified.
Synonyms
v-Myb/MYBL1, Antibody; Anti-v-Myb/MYBL1 Antibody; MYBL1; AMYB; Myb-related protein A; A-Myb; Myb-like protein 1; MYB proto-oncogene like 1; anti-MYBL1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Rabbit IgG polyclonal antibody for v-Myb/MYBL1 detection.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Applicable Applications for anti-MYBL1 antibody
ELISA (Direct ELISA), FCM/FACS (Flow Cytometry), WB (Western Blot)
Immunogen
E Coli-derived human v-Myb/MYBL1 recombinant protein (Position: K199-L752).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Recommended Detection Systems
Recommended Detection Systems
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of U87 cells using anti-v-Myb/MYBL1 antibody (AAA125759).Overlay histogram showing U87 cells stained with AAA125759 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-v-Myb/MYBL1 Antibody (AAA125759, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of U87 cells using anti-v-Myb/MYBL1 antibody (AAA125759).Overlay histogram showing U87 cells stained with AAA125759 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-v-Myb/MYBL1 Antibody (AAA125759, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
WB (Western Blot)
(Figure 1. Western blot analysis of v-Myb/MYBL1 using anti-v-Myb/MYBL1 antibody (AAA125759).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human T-47D whole cell lysatesLane 2: human A431 whole cell lysatesLane 3: human PC-3 whole cell lysatesLane 4: human U-87MG whole cell lysatesLane 5: human A549 whole cell lysatesLane 6: rat stomach tissue lysatesLane 7: mouse stomach tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-v-Myb/MYBL1 antigen affinity purified polyclonal antibody (Catalog # AAA125759) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for v-Myb/MYBL1 at approximately 86KD. The expected band size for v-Myb/MYBL1 is at 86KD.)
WB (Western Blot)
(Figure 1. Western blot analysis of v-Myb/MYBL1 using anti-v-Myb/MYBL1 antibody (AAA125759).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human T-47D whole cell lysatesLane 2: human A431 whole cell lysatesLane 3: human PC-3 whole cell lysatesLane 4: human U-87MG whole cell lysatesLane 5: human A549 whole cell lysatesLane 6: rat stomach tissue lysatesLane 7: mouse stomach tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-v-Myb/MYBL1 antigen affinity purified polyclonal antibody (Catalog # AAA125759) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for v-Myb/MYBL1 at approximately 86KD. The expected band size for v-Myb/MYBL1 is at 86KD.)
Related Product Information for anti-MYBL1 antibody
MYB proto-oncogene like 1 is a protein that in humans is encoded by the MYBL1 gene. A-Myb is expressed at specific times in reproductive tissues, some neural cells, and a subset of normal and neoplastic B lymphocytes. Both A-Myb and B-Myb are expressed in t(14:18) lymphoma cells where they then inhibit cell arrest and apoptotic signaling. Expression of B-Myb rescues cells from p53-induced G1 phase arrest that is mediated by p21, while A-Myb functions as an anti-apoptotic factor by effectively activating the bcl-2 promoter and thereby up-regulating Bcl-2 expression.
References
1. Barletta, C., Druck, T., LaForgia, S., Calabretta, B., Drabkin, H., Patterson, D., Croce, C. M., Huebner, K. Chromosome locations of the MYB related genes, AMYB and BMYB. Cancer Res. 51: 3821-3824, 1991.
2. Nomura, N., Takahashi, M., Matsui, M., Ishii, S., Date, T., Sasamoto, S., Ishizaki, R. Isolation of human cDNA clones of MYB-related genes, A-MYB and B-MYB. Nucleic Acids Res. 16: 11075-11089, 1988. Note: Erratum: Nucleic Acids Res. 17: 1282 only, 1989.
3. Takahashi, T., Nakagoshi, H., Sarai, A., Nomura, N., Yamamoto, T., Ishii, S. Human A-myb gene encodes a transcriptional activator containing the negative regulatory domains. FEBS Lett. 358: 89-96, 1995.
2. Nomura, N., Takahashi, M., Matsui, M., Ishii, S., Date, T., Sasamoto, S., Ishizaki, R. Isolation of human cDNA clones of MYB-related genes, A-MYB and B-MYB. Nucleic Acids Res. 16: 11075-11089, 1988. Note: Erratum: Nucleic Acids Res. 17: 1282 only, 1989.
3. Takahashi, T., Nakagoshi, H., Sarai, A., Nomura, N., Yamamoto, T., Ishii, S. Human A-myb gene encodes a transcriptional activator containing the negative regulatory domains. FEBS Lett. 358: 89-96, 1995.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
85,887 Da
NCBI Official Full Name
myb-related protein A isoform 1
NCBI Official Synonym Full Names
v-myb myeloblastosis viral oncogene homolog (avian)-like 1
NCBI Official Symbol
MYBL1
NCBI Official Synonym Symbols
AMYB; A-MYB
NCBI Protein Information
myb-related protein A; myb-like protein 1; v-myb avian myeloblastosis viral oncogene homolog-like 1
UniProt Protein Name
Myb-related protein A
UniProt Gene Name
MYBL1
UniProt Synonym Gene Names
AMYB; A-Myb
UniProt Entry Name
MYBA_HUMAN
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Product Notes
The MYBL1 mybl1 (Catalog #AAA125759) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-v-Myb/MYBL1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's v-Myb/MYBL1 can be used in a range of immunoassay formats including, but not limited to, ELISA (Direct ELISA), FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the MYBL1 mybl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "v-Myb/MYBL1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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