WB (Western Blot)
(Anti-ABCB1 Antibody - Western Blot. Western blot of affinity purified anti-ABCB1 antibody shows detection of ABCB1 in crude membrane extracts from HF insect cells over-expressing human ABCB1. The extract was loaded onto a gel in the amounts indicated followed by electrophoresis and transfer to nitrocellulose. The membrane was probed with the primary antibody diluted to 1:600, followed by Peroxidase Conjugated Affinity Purified Anti-RABBIT IgG at 1:10000. Personal Communication, Anna Calcagno, CCR-NCI, Bethesda, MD.)
Rabbit ABCB1 / MDR1 / P Glycoprotein Polyclonal Antibody | anti-ABCB1 antibody
Rabbit Polyclonal to Human ABCB1 / MDR1 / P Glycoprotein
Gene Names
ABCB1; CLCS; MDR1; P-GP; PGY1; ABC20; CD243; GP170
Reactivity
Dog, Human, Monkey, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Immunoaffinity Purified
Synonyms
ABCB1 / MDR1 / P Glycoprotein, Antibody; Rabbit Polyclonal to Human ABCB1 / MDR1 / P Glycoprotein; Anti-ABCB1 / MDR1 / P Glycoprotein Antibody (aa262-277) IHC-plus; ABCB1; ABC20; Abcb1b; CLCS; Colchicin sensitivity; CD243; Doxorubicin resistance; IBD13; gp170; MDR1; Multidrug resistance protein 1; P glycoprotein; P-glycoprotein 1; P-GP; CD243 antigen; PGY1; Human ABCB1; P Glycoprotein; anti-ABCB1 antibody
Host
Rabbit
Reactivity
Dog, Human, Monkey, Mouse, Rat
Clonality
Polyclonal
Specificity
Amino acids 262-277 of human ABCB1 protein.
Purity/Purification
Immunoaffinity Purified
Form/Format
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Concentration
1 mg/ml (varies by lot)
Sequence Length
1280
Applicable Applications for anti-ABCB1 antibody
ELISA, WB (Western Blot), IHC (Immunohistochemistry)
Target Species
Human
Immunogen Description
Synthetic peptide from human ABCB1 / P Glycoprotein.
Immunogen Type
Synthetic peptide
Immunogen
ABCB1 / MDR1 / P Glycoprotein antibody was raised against synthetic peptide from human ABCB1 / P Glycoprotein.
Antigen Modification
aa262-277
Preparation and Storage
Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.
WB (Western Blot)
(Anti-ABCB1 Antibody - Western Blot. Western blot of affinity purified anti-ABCB1 antibody shows detection of ABCB1 in crude membrane extracts from HF insect cells over-expressing human ABCB1. The extract was loaded onto a gel in the amounts indicated followed by electrophoresis and transfer to nitrocellulose. The membrane was probed with the primary antibody diluted to 1:600, followed by Peroxidase Conjugated Affinity Purified Anti-RABBIT IgG at 1:10000. Personal Communication, Anna Calcagno, CCR-NCI, Bethesda, MD.)
WB (Western Blot)
(Anti-ABCB1 Antibody - Western Blot. Western blot of affinity purified anti-ABCB1 antibody shows detection of ABCB1 in crude membrane extracts from HF insect cells over-expressing human ABCB1. The extract was loaded onto a gel in the amounts indicated followed by electrophoresis and transfer to nitrocellulose. The membrane was probed with the primary antibody diluted to 1:600, followed by Peroxidase Conjugated Affinity Purified Anti-RABBIT IgG at 1:10000. Personal Communication, Anna Calcagno, CCR-NCI, Bethesda, MD.)
IHC (Immunohistochemistry)
(Anti-MDR1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
IHC (Immunohistochemistry)
(Anti-MDR1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Related Product Information for anti-ABCB1 antibody
ABCB1/MDR1/P Glycoprotein is an ATP dependent transporter that mediates efflux of chemotherapeutic agents from the intracellular milieu. P Glycoprotein has also been associated with volume-regulated chloride channel activity and basal to apical transendothelial migration of mononuclear phagocytes.
Product Categories/Family for anti-ABCB1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
134,032 Da
NCBI Official Full Name
multidrug resistance protein 1
NCBI Official Synonym Full Names
ATP-binding cassette, sub-family B (MDR/TAP), member 1
NCBI Official Symbol
ABCB1
NCBI Official Synonym Symbols
CLCS; MDR1; P-GP; PGY1; ABC20; CD243; GP170
NCBI Protein Information
multidrug resistance protein 1; P-glycoprotein 1; colchicin sensitivity; doxorubicin resistance
UniProt Protein Name
Multidrug resistance protein 1
UniProt Gene Name
ABCB1
UniProt Synonym Gene Names
MDR1; PGY1
UniProt Entry Name
MDR1_HUMAN
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Product Notes
The ABCB1 abcb1 (Catalog #AAA50807) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal to Human ABCB1 / MDR1 / P Glycoprotein reacts with Dog, Human, Monkey, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ABCB1 / MDR1 / P Glycoprotein can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the ABCB1 abcb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ABCB1 / MDR1 / P Glycoprotein, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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