WB (Western Blot)
(Detection of human MDR1 by western blot. Samples: Whole cell lysate (25 ug) from HeLa, Hep-G2, Jurkat, HEK293T, and U2OS cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MDR1 antibody (AAA212240 lot 2) used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human MDR1/ABCB1 Polyclonal Antibody | anti-MDR1/ABCB1 antibody
Rabbit anti-MDR1/ABCB1 Antibody, Affinity Purified
Gene Names
ABCB1; CLCS; MDR1; P-GP; PGY1; ABC20; CD243; GP170
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
MDR1/ABCB1, Antibody; Rabbit anti-MDR1/ABCB1 Antibody, Affinity Purified; GP170; ABC20; P-GP; P-glycoprotein 1; P glycoprotein; multidrug resistance protein 1; MDR1; doxorubicin resistance; colchicin sensitivity; CLCS; CD243; CD antigen CD243; ATP-binding cassette sub-family B member 1; PGY1; ATP-binding cassette, sub-family B (MDR/TAP), member 1; Multidrug resistance protein 1; ATP binding cassette subfamily B member 1; anti-MDR1/ABCB1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-MDR1/ABCB1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human MDR1 by western blot. Samples: Whole cell lysate (25 ug) from HeLa, Hep-G2, Jurkat, HEK293T, and U2OS cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MDR1 antibody (AAA212240 lot 2) used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human MDR1 by western blot. Samples: Whole cell lysate (25 ug) from HeLa, Hep-G2, Jurkat, HEK293T, and U2OS cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MDR1 antibody (AAA212240 lot 2) used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human MDR1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Hep-G2 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MDR1 antibody (AAA212240 lot 2) used for IP at 6 ug per reaction. MDR1 was also immunoprecipitated by a previous lot of this antibody (AAA212240 lot 1) and a second antibody against a different epitope of MDR1 (BL12936). For blotting immunoprecipitated MDR1, AAA212240 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human MDR1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Hep-G2 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MDR1 antibody (AAA212240 lot 2) used for IP at 6 ug per reaction. MDR1 was also immunoprecipitated by a previous lot of this antibody (AAA212240 lot 1) and a second antibody against a different epitope of MDR1 (BL12936). For blotting immunoprecipitated MDR1, AAA212240 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-MDR1/ABCB1 antibody
Multidrug resistance protein 1 (MDR1) is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, and White). MDR1 is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. MDR1 is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. MDR1 also functions as a transporter in the blood-brain barrier [taken from NCBI Entrez Gene (Gene ID: 5243)].
Product Categories/Family for anti-MDR1/ABCB1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
141kDa
NCBI Official Full Name
multidrug resistance protein 1
NCBI Official Synonym Full Names
ATP-binding cassette, sub-family B (MDR/TAP), member 1
NCBI Official Symbol
ABCB1
NCBI Official Synonym Symbols
CLCS; MDR1; P-GP; PGY1; ABC20; CD243; GP170
NCBI Protein Information
multidrug resistance protein 1; P-glycoprotein 1; colchicin sensitivity; doxorubicin resistance
UniProt Protein Name
Multidrug resistance protein 1
UniProt Gene Name
ABCB1
UniProt Synonym Gene Names
MDR1; PGY1
UniProt Entry Name
MDR1_HUMAN
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Product Notes
The MDR1/ABCB1 abcb1 (Catalog #AAA212240) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-MDR1/ABCB1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MDR1/ABCB1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the MDR1/ABCB1 abcb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MDR1/ABCB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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