WB (Western Blot)
(Detection of human ACE2 by western blot. Samples: Whole cell lysate (5 ug) from NCI-H226, IMR-90, OV-90, HDLM-2, and A-549 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ACE2 antibody (AAA213492 lot 1) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
Rabbit anti-Human ACE2 Polyclonal Antibody | anti-ACE2 antibody
Rabbit anti-ACE2 Antibody, Affinity Purified
Gene Names
ACE2; ACEH
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
ACE2, Antibody; Rabbit anti-ACE2 Antibody, Affinity Purified; ACE2(18-740)/Rb-Fc; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; peptidyl-dipeptidase A; metalloprotease MPROT15; angiotensin-converting enzyme 2; ACE-related carboxypeptidase; ACEH; angiotensin-converting enzyme homolog; Angiotensin-converting enzyme 2; angiotensin I converting enzyme 2; anti-ACE2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-ACE2 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
HEK293-derived human ACE2 Gln18-Ser740, with a C-terminal Myc/TEV/HIS6/Rb-Fc Tag
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human ACE2 by western blot. Samples: Whole cell lysate (5 ug) from NCI-H226, IMR-90, OV-90, HDLM-2, and A-549 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ACE2 antibody (AAA213492 lot 1) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
WB (Western Blot)
(Detection of human ACE2 by western blot. Samples: Whole cell lysate (5 ug) from NCI-H226, IMR-90, OV-90, HDLM-2, and A-549 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ACE2 antibody (AAA213492 lot 1) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
IP (Immunoprecipitation)
(Detection of human ACE2 by western blot of immunoprecipitates. Samples: Whole cell lysate (500 ug per IP reaction; 5% of IP loaded) from OV-90 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ACE2 antibody (AAA213492 lot 1) used for IP at 6 ug per reaction. ACE2 was also immunoprecipitated by a second antibody against a different epitope to ACE2 (Antibody 2). For blotting immunoprecipitated ACE2, AAA213492 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
IP (Immunoprecipitation)
(Detection of human ACE2 by western blot of immunoprecipitates. Samples: Whole cell lysate (500 ug per IP reaction; 5% of IP loaded) from OV-90 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ACE2 antibody (AAA213492 lot 1) used for IP at 6 ug per reaction. ACE2 was also immunoprecipitated by a second antibody against a different epitope to ACE2 (Antibody 2). For blotting immunoprecipitated ACE2, AAA213492 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
Related Product Information for anti-ACE2 antibody
ACE2 acts as a receptor for human coronaviruses SARS-CoV and SARS-CoV-2, as well as human coronavirus NL63/HCoV-NL63. ACE2 is also an essential counter-regulatory carboxypeptidase of the renin-angiotensin hormone system that is a critical regulator of blood volume, systemic vascular resistance, and thus cardiovascular homeostasis. (Uniprot)
Product Categories/Family for anti-ACE2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
92,463 Da
NCBI Official Full Name
angiotensin-converting enzyme 2
NCBI Official Synonym Full Names
angiotensin I converting enzyme 2
NCBI Official Symbol
ACE2
NCBI Official Synonym Symbols
ACEH
NCBI Protein Information
angiotensin-converting enzyme 2; peptidyl-dipeptidase A; metalloprotease MPROT15; ACE-related carboxypeptidase; angiotensin-converting enzyme homolog; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
UniProt Protein Name
Angiotensin-converting enzyme 2
UniProt Gene Name
ACE2
UniProt Synonym Gene Names
ACEH
UniProt Entry Name
ACE2_HUMAN
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Product Notes
The ACE2 ace2 (Catalog #AAA213492) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-ACE2 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ACE2 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the ACE2 ace2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ACE2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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