Sheep anti-Human ACHE / Acetylcholinesterase Polyclonal Antibody | anti-ACHE antibody

Sheep Polyclonal (IgG) to Human ACHE / Acetylcholinesterase

Cat. Num. AAA51200

• Gene Names: ACHE; YT; ACEE; ARACHE; N-ACHE
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Protein G Purified
• Synonyms: ACHE / Acetylcholinesterase, Antibody; Sheep Polyclonal (IgG) to Human ACHE / Acetylcholinesterase; Anti-ACHE / Acetylcholinesterase Antibody IHC-plus; ACHE; ACEE; Acetylcholinesterase; N-ACHE; Yt blood group; ARACHE; YT; Human ACHE; anti-ACHE antibody

• Host: Sheep
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Acetylcholinesterase.
• Purity/Purification: Protein G Purified
• Form/Format: PBS, 0.09% sodium azide
• Concentration: 5 mg/ml (varies by lot)
• Sequence Length: 617

• Applicable Applications for anti-ACHE antibody: ELISA, IHC (Immunohistochemistry)
• Target Species: Human
• Antigen Type: Native protein
• Immunogen: ACHE / Acetylcholinesterase antibody was raised against native protein.
• Immunogen Species: ACHE / Acetylcholinesterase antibody was produced against Human.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.

Related Product Information for anti-ACHE antibody

Acetylcholinesterase is involved in termination of signal transmission at neuromuscular junctions. Differences in assembly and localization of the multiple synaptic forms of acetylcholinesterase are thought to arise through posttranscriptional events. The Yt erythrocyte blood group antigen system resides on the acetylcholinesterase molecule.

NCBI and Uniprot Product Information

• NCBI GI #: 7710112
• NCBI GeneID: 43
• NCBI Accession #: NP_056646.1
• NCBI GenBank Nucleotide #: NM_015831.2
• UniProt Accession #: P22303; Q16169; Q29S23; Q2M324; Q504V3; Q53F46; Q86TM9; Q86YX9; Q9BXP7; A4D2E2; B7ZKZ0; D6W5X7
• NCBI Official Full Name: acetylcholinesterase isoform E4-E5
• NCBI Official Synonym Full Names: acetylcholinesterase (Yt blood group)
• NCBI Official Symbol: ACHE
• NCBI Official Synonym Symbols: YT; ACEE; ARACHE; N-ACHE
• NCBI Protein Information: acetylcholinesterase; Yt blood group; apoptosis-related acetylcholinesterase
• UniProt Protein Name: Acetylcholinesterase
• UniProt Gene Name: ACHE
• UniProt Synonym Gene Names: AChE
• UniProt Entry Name: ACES_HUMAN