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IF (Immunofluorescence)
(Confocal immunofluorescent analysis of ACHE Antibody (N-term) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
Rabbit ACHE Polyclonal Antibody | anti-ACHE antibody
ACHE Antibody (N-term)
Gene Names
ACHE; YT; ACEE; ARACHE; N-ACHE
Reactivity
Human (Predicted Reactivity: Guinea Pig)
Applications
Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ACHE, Antibody; ACHE Antibody (N-term); Acetylcholinesterase; AChE; ACHE; anti-ACHE antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Guinea Pig)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ACHE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 147-175 amino acids from the N-terminal region of human ACHE.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
147-175
Sequence Length
526
Applicable Applications for anti-ACHE antibody
WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of ACHE Antibody (N-term) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of ACHE Antibody (N-term) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
FCM/FACS (Flow Cytometry)
(ACHE Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(ACHE Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with ACHE antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with ACHE antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(ACHE Antibody (N-term) western blot analysis in Jurkat,Raji,Y79 cell line lysates (35ug/lane).This demonstrates the ACHE antibody detected the ACHE protein (arrow).)
WB (Western Blot)
(ACHE Antibody (N-term) western blot analysis in Jurkat,Raji,Y79 cell line lysates (35ug/lane).This demonstrates the ACHE antibody detected the ACHE protein (arrow).)
Related Product Information for anti-ACHE antibody
Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. The Protein is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits.
Product Categories/Family for anti-ACHE antibody
References
Liang,D., FEBS J. 276 (1), 94-108 (2009)
Scacchi,R., Am. J. Med. Genet. B Neuropsychiatr. Genet. (2008)
Scacchi,R., Am. J. Med. Genet. B Neuropsychiatr. Genet. (2008)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
67796
NCBI Official Full Name
acetylcholinesterase isoform E4-E6
NCBI Official Synonym Full Names
acetylcholinesterase (Yt blood group)
NCBI Official Symbol
ACHE
NCBI Official Synonym Symbols
YT; ACEE; ARACHE; N-ACHE
NCBI Protein Information
acetylcholinesterase
UniProt Protein Name
Acetylcholinesterase
UniProt Gene Name
ACHE
UniProt Synonym Gene Names
AChE
UniProt Entry Name
ACES_HUMAN
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Product Notes
The ACHE ache (Catalog #AAA287887) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 147-175. The ACHE Antibody (N-term) reacts with Human (Predicted Reactivity: Guinea Pig) and may cross-react with other species as described in the data sheet. AAA Biotech's ACHE can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the ACHE ache for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ACHE, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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