WB (Western Blot)
(Anti-Acid Sphingomyelinase Antibodyat 1:1000 dilution + HL-60 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 70 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit Acid Sphingomyelinase Polyclonal Antibody | anti-SMPD1 antibody
Acid Sphingomyelinase Antibody
Gene Names
SMPD1; ASM; NPD; ASMASE
Reactivity
Human, mouse, rat
Applications
ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Acid Sphingomyelinase, Antibody; Acid Sphingomyelinase Antibody; Sphingomyelin phosphodiesterase; Acid sphingomyelinase; aSMase; SMPD1; ASM; anti-SMPD1 antibody
Host
Rabbit
Reactivity
Human, mouse, rat
Clonality
Polyclonal
Specificity
KLH conjugated synthetic peptide derived from human Acid Sphingomyelinase
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
0.01M PBS, pH 7.2, 0.09% (W/V) Sodium azide, Glycerol 50%
Sequence Positions
421-480
Sequence Length
628
Applicable Applications for anti-SMPD1 antibody
ELISA, WB (Western Blot)
Function
Converts sphingomyelin to ceramide (PubMed: , PubMed: , PubMed: , PubMed: , PubMed: , PubMed: , PubMed: ). Exists as two enzymatic forms that arise from alternative trafficking of a single protein precursor, one that is targeted to the endolysosomal compartment, whereas the other is released extracellularly (PubMed:21098024, PubMed:9660788, PubMed:20807762). However, in response to various forms of stress, lysosomal exocytosis may represent a major source of the secretory form (PubMed: , PubMed: , PubMed: , PubMed: , PubMed: ).
Cellular Location
Lysosome. Lipid droplet. Secreted. Note=The secreted form is induced in a time- and dose-dependent by IL1B and TNF as well as stress and viral infection. This increase of the secreted form seems to be due to exocytosis of the lysosomal form and is Ca(2+)-dependent (PubMed:20807762, PubMed:22573858, PubMed:20530211). Secretion is dependent of phosphorylation at Ser-510 (PubMed:17303575). Secretion is induced by inflammatory mediators such as IL1B, IFNG or TNF as well as infection with bacteria and viruses (PubMed:12563314, PubMed:20807762)
Preparation and Storage
Store at -20 °C. Stable for 12 months from date of receipt
WB (Western Blot)
(Anti-Acid Sphingomyelinase Antibodyat 1:1000 dilution + HL-60 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 70 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-Acid Sphingomyelinase Antibodyat 1:1000 dilution + HL-60 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 70 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-SMPD1 antibody
Converts sphingomyelin to ceramide. Also has phospholipase C activities toward 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol. Isoform 2 and isoform 3 have lost catalytic activity.
Product Categories/Family for anti-SMPD1 antibody
References
Schuchman E.H.,et al.J. Biol. Chem. 266:8531-8539(1991).
Newrzella D.,et al.Biol. Chem. Hoppe-Seyler 373:1233-1238(1992).
Schuchman E.H.,et al.Genomics 12:197-205(1992).
Ida H.,et al.J. Biochem. 114:15-20(1993).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Newrzella D.,et al.Biol. Chem. Hoppe-Seyler 373:1233-1238(1992).
Schuchman E.H.,et al.Genomics 12:197-205(1992).
Ida H.,et al.J. Biochem. 114:15-20(1993).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
70 kDa
NCBI Official Full Name
sphingomyelin phosphodiesterase isoform 1
NCBI Official Synonym Full Names
sphingomyelin phosphodiesterase 1, acid lysosomal
NCBI Official Symbol
SMPD1
NCBI Official Synonym Symbols
ASM; NPD; ASMASE
NCBI Protein Information
sphingomyelin phosphodiesterase
UniProt Protein Name
Sphingomyelin phosphodiesterase
UniProt Gene Name
SMPD1
UniProt Synonym Gene Names
ASM; aSMase
UniProt Entry Name
ASM_HUMAN
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Product Notes
The SMPD1 smpd1 (Catalog #AAA289658) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 421-480. The Acid Sphingomyelinase Antibody reacts with Human, mouse, rat and may cross-react with other species as described in the data sheet. AAA Biotech's Acid Sphingomyelinase can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the SMPD1 smpd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Acid Sphingomyelinase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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