Rabbit ACKR3 / CXCR7 / RDC1 Polyclonal Antibody | anti-ACKR3 antibody

Rabbit Polyclonal (IgG) to Human ACKR3 / CXCR7

Cat. Num. AAA50840

• Gene Names: ACKR3; RDC1; CXCR7; RDC-1; CMKOR1; CXC-R7; CXCR-7; GPR159
• Reactivity: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit; Predicted Reactivity: Xenopus
• Applications: Flow Cytometry, Functional Assay, Western Blot, Immunohistochemistry
• Purity: Protein G Purified
• Synonyms: ACKR3 / CXCR7 / RDC1, Antibody; Rabbit Polyclonal (IgG) to Human ACKR3 / CXCR7; Anti-ACKR3 / CXCR7 / RDC1 Antibody (aa100-150) IHC-plus; ACKR3; Chemokine orphan receptor 1; CMKOR1; CXCR7; CXC-R7; GPRN1; RDC1; RDC-1; CXCR-7; G-protein coupled receptor 159; GPR159; Human ACKR3; anti-ACKR3 antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit; Predicted Reactivity: Xenopus
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: A portion of amino acids 100-150 of human RDC1
• Purity/Purification: Protein G Purified
• Form/Format: Tris-Glycine, 0.05% Sodium Azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 362

• Applicable Applications for anti-ACKR3 antibody: FCM/FACS (Flow Cytometry), WB (Western Blot), IHC (Immunohistochemistry)
• Immmunogen Species: Human
• Immunogen Type: Synthetic peptide
• Immunogen: ACKR3 / CXCR7 antibody was raised against a synthetic peptide (QHNQWPMGELTC) corresponding amino acids 106-117 of human CXCR7/RDC1 was used as the immunogen, GenBank NP_064707.1. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Bat, Dog, Horse, Rabbit, Pig, Opossum (100%); Platypus, Xenopus (92%); Turkey (83%).
• Epitope: aa100-150
• Target: ACKR3/CXCR7
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

WB (Western Blot)

(Western blot of human CXCR7/RDC1 in Jurkat cell lysate in the 1) absence and 2) presence of immunizing peptide using at 5 ug/ml.)

IHC (Immunohistochemistry)

(Anti-CXCR7 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Product Categories/Family for anti-ACKR3 antibody

Family: GPCR; Subfamily: Chemokine

NCBI and Uniprot Product Information

• NCBI GI #: 31083344
• NCBI GeneID: 57007
• NCBI Accession #: NP_064707.1
• NCBI GenBank Nucleotide #: NM_020311.2
• UniProt Accession #: P25106; Q53RV4; Q8NE10; Q92938; Q92986; A8K6J4
• NCBI Official Full Name: atypical chemokine receptor 3
• NCBI Official Synonym Full Names: atypical chemokine receptor 3
• NCBI Official Symbol: ACKR3
• NCBI Official Synonym Symbols: RDC1; CXCR7; RDC-1; CMKOR1; CXC-R7; CXCR-7; GPR159
• NCBI Protein Information: atypical chemokine receptor 3; G protein-coupled receptor; chemokine orphan receptor 1; G-protein coupled receptor 159; C-X-C chemokine receptor type 7; chemokine (C-X-C motif) receptor 7; G-protein coupled receptor RDC1 homolog
• UniProt Protein Name: Atypical chemokine receptor 3
• UniProt Gene Name: ACKR3
• UniProt Synonym Gene Names: CMKOR1; CXCR7; GPR159; RDC1; CXC-R7; CXCR-7; RDC-1
• UniProt Entry Name: ACKR3_HUMAN