Rabbit anti-Human CMKOR1 Polyclonal Antibody | anti-ACKR3 antibody

CMKOR1 Antibody (C-term)

Cat. Num. AAA286482

• Gene Names: ACKR3; RDC1; CXCR7; RDC-1; CMKOR1; CXC-R7; CXCR-7; GPR159
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA, Western Blot
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: CMKOR1, Antibody; CMKOR1 Antibody (C-term); Atypical chemokine receptor 3; C-X-C chemokine receptor type 7; CXC-R7; CXCR-7; Chemokine orphan receptor 1; G-protein coupled receptor 159; G-protein coupled receptor RDC1 homolog; RDC-1; ACKR3; CMKOR1; CXCR7; GPR159; RDC1; anti-ACKR3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: This CMKOR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 333-362 amino acids from the C-terminal region of human CMKOR1.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Positions: 333-362
• Sequence Length: 362

• Applicable Applications for anti-ACKR3 antibody: FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
• Function: Atypical chemokine receptor that controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades, resulting instead in chemokine sequestration, degradation, or transcytosis. Also known as interceptor (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor. Acts as a receptor for chemokines CXCL11 and CXCL12/SDF1 (PubMed:16107333, PubMed:19255243, PubMed:19380869, PubMed:20161793, PubMed:22300987). Chemokine binding does not activate G-protein-mediated signal transduction but instead induces beta-arrestin recruitment, leading to ligand internalization and activation of MAPK signaling pathway (PubMed:16940167, PubMed:18653785, PubMed:20018651). Required for regulation of CXCR4 protein levels in migrating interneurons, thereby adapting their chemokine responsiveness (PubMed:16940167, PubMed:18653785). In glioma cells, transduces signals via MEK/ERK pathway, mediating resistance to apoptosis. Promotes cell growth and survival (PubMed:16940167, PubMed:20388803). Not involved in cell migration, adhesion or proliferation of normal hematopoietic progenitors but activated by CXCL11 in malignant hemapoietic cells, leading to phosphorylation of ERK1/2 (MAPK3/MAPK1) and enhanced cell adhesion and migration (PubMed:17804806, PubMed:18653785, PubMed:19641136, PubMed:20887389). Plays a regulatory role in CXCR4-mediated activation of cell surface integrins by CXCL12 (PubMed:18653785). Required for heart valve development (PubMed:17804806). Regulates axon guidance in the oculomotor system through the regulation of CXCL12 levels (PubMed:31211835).
• Cellular Location: Cell membrane; Multi-pass membrane protein. Early endosome. Recycling endosome. Note=Predominantly localizes to endocytic vesicles, and upon stimulation by the ligand is internalized via clathrin-coated pits in a beta-arrestin-dependent manner. Once internalized, the ligand dissociates from the receptor, and is targeted to degradation while the receptor is recycled back to the cell membrane.
• Tissue Location: Expressed in monocytes, basophils, B-cells, umbilical vein endothelial cells (HUVEC) and B-lymphoblastoid cells Lower expression detected in CD4+ T-lymphocytes and natural killer cells. In the brain, detected in endothelial cells and capillaries, and in mature neurons of the frontal cortex and hippocampus. Expressed in tubular formation in the kidney. Highly expressed in astroglial tumor endothelial, microglial and glioma cells. Expressed at low levels in normal CD34+ progenitor cells, but at very high levels in several myeloid malignant cell lines. Expressed in breast carcinomas but not in normal breast tissue (at protein level).
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(CMKOR1 Antibody (C-term) (Cat. # AAA286482) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

WB (Western Blot)

(CMKOR1 Antibody (C-term) (Cat. # AAA286482) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the CMKOR1 antibody detected the CMKOR1 protein (arrow).)

Related Product Information for anti-ACKR3 antibody

CXCR7 encodes a member of the G-protein coupled receptor family. Although this protein was earlier thought to be a receptor for vasoactive intestinal peptide (VIP), it is now considered to be an orphan receptor, in that its endogenous ligand has not been identified. The protein is also a coreceptor for human immunodeficiency viruses (HIV). Translocations involving this gene and HMGA2 on chromosome 12 have been observed in lipomas. [provided by RefSeq].

Product Categories/Family for anti-ACKR3 antibody

Signal Transduction; GPCR Antibodies

References

Berahovich, R.D., et al. J. Immunol. 185(9):5130-5139(2010)
Watanabe, K., et al. Arthritis Rheum. 62(11):3211-3220(2010)
Hattermann, K., et al. Cancer Res. 70(8):3299-3308(2010)
Miekus, K., et al. Folia Histochem. Cytobiol. 48(1):104-111(2010)
Zheng, K., et al. J. Exp. Clin. Cancer Res. 29, 31 (2010) :

NCBI and Uniprot Product Information

• NCBI GI #: 31083344
• NCBI GeneID: 57007
• NCBI Accession #: NP_064707.1
• NCBI GenBank Nucleotide #: NM_020311.2
• UniProt Accession #: P25106; Q53RV4; Q8NE10; Q92938; Q92986; A8K6J4
• Molecular Weight: Calculated MW: 41493
• NCBI Official Full Name: atypical chemokine receptor 3
• NCBI Official Synonym Full Names: atypical chemokine receptor 3
• NCBI Official Symbol: ACKR3
• NCBI Official Synonym Symbols: RDC1; CXCR7; RDC-1; CMKOR1; CXC-R7; CXCR-7; GPR159
• NCBI Protein Information: atypical chemokine receptor 3
• UniProt Protein Name: Atypical chemokine receptor 3
• UniProt Gene Name: ACKR3
• UniProt Synonym Gene Names: CMKOR1; CXCR7; GPR159; RDC1; CXC-R7; CXCR-7; RDC-1
• UniProt Entry Name: ACKR3_HUMAN