FCM/FACS (Flow Cytometry)
(ACOT11 Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human ACOT11 Polyclonal Antibody | anti-ACOT11 antibody
ACOT11 Antibody (C-term)
Gene Names
ACOT11; BFIT; THEA; THEM1; STARD14
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ACOT11, Antibody; ACOT11 Antibody (C-term); Acyl-coenzyme A thioesterase 11; Acyl-CoA thioesterase 11; 312-; Acyl-CoA thioester hydrolase 11; Adipose-associated thioesterase; Brown fat-inducible thioesterase; BFIT; ACOT11; KIAA0707; THEA; anti-ACOT11 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ACOT11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 549-575 amino acids from the C-terminal region of human ACOT11.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
549-575
Sequence Length
607
Applicable Applications for anti-ACOT11 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(ACOT11 Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(ACOT11 Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(ACOT11 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ACOT11 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(ACOT11 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ACOT11 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(ACOT11 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the ACOT11 antibody detected the ACOT11 protein (arrow).)
WB (Western Blot)
(ACOT11 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the ACOT11 antibody detected the ACOT11 protein (arrow).)
Related Product Information for anti-ACOT11 antibody
This gene encodes a member of the acyl-CoA thioesterase
family which catalyse the conversion of activated fatty acids to
the corresponding non-esterified fatty acid and coenzyme A.
Expression of a mouse homolog in brown adipose tissue is induced by
low temperatures and repressed by warm temperatures. Higher levels
of expression of the mouse homolog has been found in
obesity-resistant mice compared with obesity-prone mice, suggesting
a role of acyl-CoA thioesterase 11 in obesity. Alternative splicing
results in transcript variants.
family which catalyse the conversion of activated fatty acids to
the corresponding non-esterified fatty acid and coenzyme A.
Expression of a mouse homolog in brown adipose tissue is induced by
low temperatures and repressed by warm temperatures. Higher levels
of expression of the mouse homolog has been found in
obesity-resistant mice compared with obesity-prone mice, suggesting
a role of acyl-CoA thioesterase 11 in obesity. Alternative splicing
results in transcript variants.
Product Categories/Family for anti-ACOT11 antibody
References
Kirkby, B., et al. Prog. Lipid Res. 49(4):366-377(2010)
Hunt, M.C., et al. J. Lipid Res. 46(9):2029-2032(2005)
Adams, S.H., et al. Biochem. J. 360 (PT 1), 135-142 (2001) :
Hunt, M.C., et al. J. Lipid Res. 46(9):2029-2032(2005)
Adams, S.H., et al. Biochem. J. 360 (PT 1), 135-142 (2001) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
68492
NCBI Official Full Name
acyl-coenzyme A thioesterase 11 isoform 1
NCBI Official Synonym Full Names
acyl-CoA thioesterase 11
NCBI Official Symbol
ACOT11
NCBI Official Synonym Symbols
BFIT; THEA; THEM1; STARD14
NCBI Protein Information
acyl-coenzyme A thioesterase 11
UniProt Protein Name
Acyl-coenzyme A thioesterase 11
UniProt Gene Name
ACOT11
UniProt Synonym Gene Names
BFIT; KIAA0707; THEA; Acyl-CoA thioesterase 11; BFIT
UniProt Entry Name
ACO11_HUMAN
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Product Notes
The ACOT11 acot11 (Catalog #AAA288754) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 549-575. The ACOT11 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ACOT11 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the ACOT11 acot11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ACOT11, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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