WB (Western Blot)
(Detection of human ACSL4 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ACSL4 antibody AAA213229 (lot AAA213229-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human ACSL4/FACL4 Polyclonal Antibody | anti-ACSL4/FACL4 antibody
Rabbit anti-ACSL4/FACL4 Antibody, Affinity Purified
Gene Names
ACSL4; ACS4; FACL4; LACS4; MRX63; MRX68
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
ACSL4/FACL4, Antibody; Rabbit anti-ACSL4/FACL4 Antibody, Affinity Purified; fatty-acid-Coenzyme A ligase, long-chain 4; long-chain acyl-CoA synthetase 4; MRX68; MRX63; long-chain-fatty-acid--CoA ligase 4; long-chain fatty-acid-Coenzyme A ligase 4; ACS4; LACS 4; lignoceroyl-CoA synthase; FACL4; acyl-CoA synthetase 4; LACS4; Long-chain-fatty-acid--CoA ligase 4; acyl-CoA synthetase long chain family member 4; anti-ACSL4/FACL4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-ACSL4/FACL4 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 250 and 300
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human ACSL4 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ACSL4 antibody AAA213229 (lot AAA213229-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human ACSL4 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ACSL4 antibody AAA213229 (lot AAA213229-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human ACSL4 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ACSL4 antibody AAA213229 (lot AAA213229-1) used for IP at 6 ug per reaction. ACSL4 was also immunoprecipitated by rabbit anti-ACSL4 antibody BL21222. For blotting immunoprecipitated ACSL4, AAA213229 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human ACSL4 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ACSL4 antibody AAA213229 (lot AAA213229-1) used for IP at 6 ug per reaction. ACSL4 was also immunoprecipitated by rabbit anti-ACSL4 antibody BL21222. For blotting immunoprecipitated ACSL4, AAA213229 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-ACSL4/FACL4 antibody
ACSL4 is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the mental retardation or Alport syndrome [taken from NCBI Entrez Gene (Gene ID: 2182)].
Product Categories/Family for anti-ACSL4/FACL4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
74,436 Da
NCBI Official Full Name
long-chain-fatty-acid--CoA ligase 4 isoform 1
NCBI Official Synonym Full Names
acyl-CoA synthetase long-chain family member 4
NCBI Official Symbol
ACSL4
NCBI Official Synonym Symbols
ACS4; FACL4; LACS4; MRX63; MRX68
NCBI Protein Information
long-chain-fatty-acid--CoA ligase 4; LACS 4; acyl-CoA synthetase 4; lignoceroyl-CoA synthase; long-chain acyl-CoA synthetase 4; long-chain fatty-acid-Coenzyme A ligase 4; fatty-acid-Coenzyme A ligase, long-chain 4
UniProt Protein Name
Long-chain-fatty-acid--CoA ligase 4
UniProt Gene Name
ACSL4
UniProt Synonym Gene Names
ACS4; FACL4; LACS4; LACS 4
UniProt Entry Name
ACSL4_HUMAN
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Product Notes
The ACSL4/FACL4 acsl4 (Catalog #AAA213229) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-ACSL4/FACL4 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ACSL4/FACL4 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the ACSL4/FACL4 acsl4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ACSL4/FACL4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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