Goat ACSL4 / FACL4 Polyclonal Antibody | anti-ACSL4 antibody

Goat Polyclonal to Human ACSL4 / FACL4

Cat. Num. AAA51630

• Gene Names: ACSL4; ACS4; FACL4; LACS4; MRX63; MRX68
• Reactivity: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: ACSL4 / FACL4, Antibody; Goat Polyclonal to Human ACSL4 / FACL4; Anti-ACSL4 / FACL4 Antibody (C-Terminus) IHC-plus; ACSL4; Acyl-CoA synthetase 4; ACS4; FACL4; LACS4; LACS 4; Lignoceroyl-CoA synthase; MRX63; MRX68; Human ACSL4; anti-ACSL4 antibody

• Host: Goat
• Reactivity: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
• Clonality: Polyclonal
• Specificity: Human ACSL4 / FACL4. This antibody is expected to recognise isoform 1 (NP_004449.1) and isoform 2 (NP_075266.1).
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 670

• Applicable Applications for anti-ACSL4 antibody: ELISA, WB (Western Blot), IHC (Immunohistochemistry)
• Immunogen Species: Human
• Epitope: aa658-670
• Immunogen Type: Synthetic peptide
• Immunogen: ACSL4 / FACL4 antibody was raised against peptide with C-HYLKDIERMYGGK, from the C-Terminus of protein sequence according to NP_004449.1 ; NP_0752661.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Aliquot and store at -20°C. Avoid freeze-thaw cycles.

WB (Western Blot)

(ACSL4 antibody (0.1 ug/ml) staining of HepG2 lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

IHC (Immunohiostchemistry)

(Anti-ACSL4 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

IHC (Immunohistochemistry)

(Anti-ACSL4 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 4758332
• NCBI GeneID: 2182
• NCBI Accession #: NP_004449.1
• NCBI GenBank Nucleotide #: NM_004458.2
• UniProt Accession #: O60488; O60848; O60849; Q5JWV8; D3DUY2
• Molecular Weight: 74,436 Da
• NCBI Official Full Name: long-chain-fatty-acid--CoA ligase 4 isoform 1
• NCBI Official Synonym Full Names: acyl-CoA synthetase long-chain family member 4
• NCBI Official Symbol: ACSL4
• NCBI Official Synonym Symbols: ACS4; FACL4; LACS4; MRX63; MRX68
• NCBI Protein Information: long-chain-fatty-acid--CoA ligase 4; LACS 4; acyl-CoA synthetase 4; lignoceroyl-CoA synthase; long-chain acyl-CoA synthetase 4; long-chain fatty-acid-Coenzyme A ligase 4; fatty-acid-Coenzyme A ligase, long-chain 4
• UniProt Protein Name: Long-chain-fatty-acid--CoA ligase 4
• UniProt Gene Name: ACSL4
• UniProt Synonym Gene Names: ACS4; FACL4; LACS4; LACS 4
• UniProt Entry Name: ACSL4_HUMAN