WB (Western Blot)
(WB Suggested Anti-ACTA1 AntibodyTitration: 1.0 ug/mlPositive Control: A549 Whole Cell)
Rabbit ACTA1 Polyclonal Antibody | anti-ACTA1 antibody
ACTA1 antibody - C-terminal region
Gene Names
ACTA1; ACTA; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; SHPM; CFTD1; CFTDM
Reactivity
Tested: Human, BaboonPredicted: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Yeast, Zebrafish
Applications
Western Blot
Purity
Affinity Purified
Synonyms
ACTA1, Antibody; ACTA1 antibody - C-terminal region; anti-ACTA1 antibody
Host
Rabbit
Reactivity
Tested: Human, Baboon
Predicted: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Yeast, Zebrafish
Predicted: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Yeast, Zebrafish
Clonality
Polyclonal
Purity/Purification
Affinity Purified
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration
0.5mg/ml (varies by lot)
Sequence
Synthetic peptide located within the following region: STMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITKQEYDEAGPSIVHR
Sequence Length
377
Applicable Applications for anti-ACTA1 antibody
WB (Western Blot)
Predicted Homology Based on Immunogen Sequence
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Yeast: 100%; Zebrafish: 100%
Protein Size (#AA)
377 Amino Acids
Protein Interactions
ABL1; TRIM63; TRIM55; CEP57; AMOT; UBC; SUMO3; CDKN2A; ACTG1; BRK1; ACTBL2; NCKAP1; LRCH3; MAPK14; DSTN; NMI; UBL4A; ABRA; ABLIM2; POLR1B; RRN3; HTRA2; PTPN22; ABLIM3; BTK; CCT2; TRAF3IP1; TCP1; SNCA; PARK2; LGALS3; SHC1; GSN; CDK2; CUL1; ISG15; YWHAZ; RE
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(WB Suggested Anti-ACTA1 AntibodyTitration: 1.0 ug/mlPositive Control: A549 Whole Cell)
WB (Western Blot)
(WB Suggested Anti-ACTA1 AntibodyTitration: 1.0 ug/mlPositive Control: A549 Whole Cell)
WB (Western Blot)
(WB Suggested Anti-ACTA1 AntibodyPositive Control: Lane 1: 50ug term baboon muscle homogenatePrimary Antibody Dilution : 1:1666Secondary Antibody : Anti-rabbit-HRPSecondry Antibody Dilution : 1:1200Submitted by: Cynthia Blanco, University of Texas Health Science Center)
WB (Western Blot)
(WB Suggested Anti-ACTA1 AntibodyPositive Control: Lane 1: 50ug term baboon muscle homogenatePrimary Antibody Dilution : 1:1666Secondary Antibody : Anti-rabbit-HRPSecondry Antibody Dilution : 1:1200Submitted by: Cynthia Blanco, University of Texas Health Science Center)
IHC (Immunohistochemistry)
(Rabbit Anti-ACTA1 AntibodyFormalin Fixed Paraffin Embedded Tissue: Human Heart Muscle TissueObserved Staining: CytoplasmPrimary Antibody Concentration: 1:100Other Working Concentrations: 1:600Secondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 - 2.0 sec)
IHC (Immunohistochemistry)
(Rabbit Anti-ACTA1 AntibodyFormalin Fixed Paraffin Embedded Tissue: Human Heart Muscle TissueObserved Staining: CytoplasmPrimary Antibody Concentration: 1:100Other Working Concentrations: 1:600Secondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 - 2.0 sec)
Related Product Information for anti-ACTA1 antibody
This is a rabbit polyclonal antibody against ACTA1. It was validated on Western Blot
Target Description: The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.
Target Description: The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.
Product Categories/Family for anti-ACTA1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
42kDa
NCBI Official Full Name
actin, alpha skeletal muscle
NCBI Official Synonym Full Names
actin alpha 1, skeletal muscle
NCBI Official Symbol
ACTA1
NCBI Official Synonym Symbols
ACTA; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; SHPM; CFTD1; CFTDM
NCBI Protein Information
actin, alpha skeletal muscle
UniProt Protein Name
Actin, alpha skeletal muscle
UniProt Gene Name
ACTA1
UniProt Synonym Gene Names
ACTA
UniProt Entry Name
ACTS_HUMAN
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Product Notes
The ACTA1 acta1 (Catalog #AAA201042) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ACTA1 antibody - C-terminal region reacts with Tested: Human, Baboon Predicted: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Yeast, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's ACTA1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the ACTA1 acta1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Synthetic peptide located within the following region: STMKIKIIAP PERKYSVWIG GSILASLSTF QQMWITKQEY DEAGPSIVHR. It is sometimes possible for the material contained within the vial of "ACTA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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