SDS-PAGE
(HEK293 (lane 2) cell lysate was run under reducing conditions on SDS PAGE and transferred to a PVDF membrane. Precision Plus ProteinTM Prestained Standards were run in lane 1. Rabbit anti catenin beta was used as the primary antibody at a dilution of 1/400 in lane 2. As secondary detection reagent HRP conjugated goat anti rabbit IgG was used at 1/10,000. Visualization was carried out using the ChemiDocTM MP Imaging System with automatic exposure)
Rabbit Actin Beta Polyclonal Antibody | anti-ACTB antibody
Rabbit anti Actin Beta
Gene Names
ACTB; BRWS1; PS1TP5BP1
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Purified by affinity chromatography
Synonyms
Actin Beta, Antibody; Rabbit anti Actin Beta; Actin Beta Antibody; anti-ACTB antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by affinity chromatography
Form/Format
Liquid in phosphate buffered saline with 0.1% sodium azide (NaN3), 0.2% gelatin.
Concentration
0.2 mg/ml (varies by lot)
Sequence Length
375
Applicable Applications for anti-ACTB antibody
WB (Western Blot)
Immunogen
Peptide mapping to the terminal domain of human actin beta
Preperation
Antiserum to actin beta was raised by repeated immunisation of rabbits with highly purified antigen
Preparation and Storage
Store at 4 degree C. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch
SDS-PAGE
(HEK293 (lane 2) cell lysate was run under reducing conditions on SDS PAGE and transferred to a PVDF membrane. Precision Plus ProteinTM Prestained Standards were run in lane 1. Rabbit anti catenin beta was used as the primary antibody at a dilution of 1/400 in lane 2. As secondary detection reagent HRP conjugated goat anti rabbit IgG was used at 1/10,000. Visualization was carried out using the ChemiDocTM MP Imaging System with automatic exposure)
SDS-PAGE
(HEK293 (lane 2) cell lysate was run under reducing conditions on SDS PAGE and transferred to a PVDF membrane. Precision Plus ProteinTM Prestained Standards were run in lane 1. Rabbit anti catenin beta was used as the primary antibody at a dilution of 1/400 in lane 2. As secondary detection reagent HRP conjugated goat anti rabbit IgG was used at 1/10,000. Visualization was carried out using the ChemiDocTM MP Imaging System with automatic exposure)
WB (Western Blot)
(Western blotting analysis of A431 whole cell lysate probed with Rabbit anti actin beta)
WB (Western Blot)
(Western blotting analysis of A431 whole cell lysate probed with Rabbit anti actin beta)
Related Product Information for anti-ACTB antibody
Rabbit anti actin beta antibody recognizes the product of the ACTB gene, cytoplasmic actin beta, also known as actin cytoplasmic and beta actin1. Actin beta is ubiquitously expressed in all eukaryotic cells, playing a critical role in cell survival, motility and re-modeling.
Actin beta is highly conserved throughout evolution, with all vertebrate and invertebrate species reviewed to date bearing a 98% or greater amino acid primary sequence identity to human actin beta. Plant species also exhibit a high degree of primary sequence conservation displaying considerable similarity to animal actin beta. For example, nearly 90% sequence identity is observed between human ACTB and the model plant species Arabidopsis thaliana (mouse eared cress) ACT1 (Arabidopsis) gene products. Actin beta is a commonly used western blotting loading control.
Actin beta is highly conserved throughout evolution, with all vertebrate and invertebrate species reviewed to date bearing a 98% or greater amino acid primary sequence identity to human actin beta. Plant species also exhibit a high degree of primary sequence conservation displaying considerable similarity to animal actin beta. For example, nearly 90% sequence identity is observed between human ACTB and the model plant species Arabidopsis thaliana (mouse eared cress) ACT1 (Arabidopsis) gene products. Actin beta is a commonly used western blotting loading control.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
41,737 Da
NCBI Official Full Name
Actin, cytoplasmic 1
NCBI Official Synonym Full Names
actin beta
NCBI Official Symbol
ACTB
NCBI Official Synonym Symbols
BRWS1; PS1TP5BP1
NCBI Protein Information
actin, cytoplasmic 1
UniProt Protein Name
Actin, cytoplasmic 1
UniProt Gene Name
ACTB
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Product Notes
The ACTB actb (Catalog #AAA50289) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti Actin Beta reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Actin Beta can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the ACTB actb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Actin Beta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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