Knockout Validation
(Knockout Validation: Lane 1: Wild-type Hela cell lysate;;Lane 2: ACTN2 knockout Hela cell lysate;;Predicted MW: 104kDa ;Observed MW: 104kDa;Primary Ab: 1ug/ml Rabbit Anti-Mouse ACTN2 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)
Rabbit anti-Human, Mouse Actinin Alpha 2 (ACTN2) Polyclonal Antibody | anti-ACTN2 antibody
Polyclonal Antibody to Actinin Alpha 2 (ACTN2)
Gene Names
Actn2; 1110008F24Rik
Reactivity
Human, Mouse
Applications
Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry
Purity
Affinity Chromatography
Synonyms
Actinin Alpha 2 (ACTN2), Antibody; Polyclonal Antibody to Actinin Alpha 2 (ACTN2); anti-ACTN2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against ACTN2. It has been selected for its ability to recognize ACTN2 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
0.48mg/ml (varies by lot)
Sequence
Antigen: The target protein is fused with a His-tag and its sequence is listed below.
MGHHHHHHSGSEF-MNQIEPGVQY NYVYDEDEYM IQEEEWDRDL LLDPAWEKQQ RKTFTAWCNS HLRKAGTQIE NIEEDFRNGL KLMLLLEVIS GERLPKPDRG KMRFHKIANV NKALDYIASK GVKLVSIGAE EIVDGNVKMT LGMIWTIILR FAIQDISVEE TSAKEGLLLW CQRKTAPYRN VNIQNFHTSW KDGLGLCALI HRHRPDLIDY SKLNKDDPIG NINLAMEIAE KHLDIPKMLD AEDIVNTPKP DERAIMTYVS CFYH
MGHHHHHHSGSEF-MNQIEPGVQY NYVYDEDEYM IQEEEWDRDL LLDPAWEKQQ RKTFTAWCNS HLRKAGTQIE NIEEDFRNGL KLMLLLEVIS GERLPKPDRG KMRFHKIANV NKALDYIASK GVKLVSIGAE EIVDGNVKMT LGMIWTIILR FAIQDISVEE TSAKEGLLLW CQRKTAPYRN VNIQNFHTSW KDGLGLCALI HRHRPDLIDY SKLNKDDPIG NINLAMEIAE KHLDIPKMLD AEDIVNTPKP DERAIMTYVS CFYH
Sequence Length
894
Applicable Applications for anti-ACTN2 antibody
WB (Western Blot), ELISA, IHC (Immunohistochemistry), ICC (Immunocytochemistry)
Immunogen
Recombinant ACTN2 (Met1~His254) expressed in E.coli.
Cross Reactivity
Human, Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Knockout Validation
(Knockout Validation: Lane 1: Wild-type Hela cell lysate;;Lane 2: ACTN2 knockout Hela cell lysate;;Predicted MW: 104kDa ;Observed MW: 104kDa;Primary Ab: 1ug/ml Rabbit Anti-Mouse ACTN2 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)
Knockout Validation
(Knockout Validation: Lane 1: Wild-type Hela cell lysate;;Lane 2: ACTN2 knockout Hela cell lysate;;Predicted MW: 104kDa ;Observed MW: 104kDa;Primary Ab: 1ug/ml Rabbit Anti-Mouse ACTN2 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)
IHC (Immunohiostchemistry)
(DAB staining on fromalin fixed paraffin-embedded heart tissue))
IHC (Immunohiostchemistry)
(DAB staining on fromalin fixed paraffin-embedded heart tissue))
WB (Western Blot)
(Western Blot: Sample: Recombinant protein.)
WB (Western Blot)
(Western Blot: Sample: Recombinant protein.)
Product Categories/Family for anti-ACTN2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
103,834 Da
NCBI Official Full Name
alpha-actinin-2
NCBI Official Synonym Full Names
actinin alpha 2
NCBI Official Symbol
Actn2
NCBI Official Synonym Symbols
1110008F24Rik
NCBI Protein Information
alpha-actinin-2
UniProt Protein Name
Alpha-actinin-2
UniProt Gene Name
Actn2
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Product Notes
The ACTN2 actn2 (Catalog #AAA131589) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Actinin Alpha 2 (ACTN2) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Actinin Alpha 2 (ACTN2) can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA, IHC (Immunohistochemistry), ICC (Immunocytochemistry). Researchers should empirically determine the suitability of the ACTN2 actn2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with a His-tag and its sequence is listed below. MGHHHHHHSG SEF-MNQIEP GVQY NYVYDEDEYM IQEEEWDRDL LLDPAWEKQQ RKTFTAWCNS HLRKAGTQIE NIEEDFRNGL KLMLLLEVIS GERLPKPDRG KMRFHKIANV NKALDYIASK GVKLVSIGAE EIVDGNVKMT LGMIWTIILR FAIQDISVEE TSAKEGLLLW CQRKTAPYRN VNIQNFHTSW KDGLGLCALI HRHRPDLIDY SKLNKDDPIG NINLAMEIAE KHLDIPKMLD AEDIVNTPKP DERAIMTYVS CFYH. It is sometimes possible for the material contained within the vial of "Actinin Alpha 2 (ACTN2), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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