FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of MDA-231 cells using ACTN4 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human ACTN4 Polyclonal Antibody | anti-ACTN4 antibody
ACTN4 Antibody (C-term)
Gene Names
ACTN4; FSGS; FSGS1; ACTININ-4
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ACTN4, Antibody; ACTN4 Antibody (C-term); Alpha-actinin-4; F-actin cross-linking protein; Non-muscle alpha-actinin 4; ACTN4; anti-ACTN4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ACTN4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 785-813 amino acids from the C-terminal region of human ACTN4.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
785-813
Sequence Length
911
Applicable Applications for anti-ACTN4 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of MDA-231 cells using ACTN4 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of MDA-231 cells using ACTN4 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human breast carcinoma reacted with ACTN4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human breast carcinoma reacted with ACTN4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of ACTN4 Antibody (C-term) in T47D, A2058, A375, HepG2 cell line and mouse bladder tissue lysates (35ug/lane). ACTN4 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of ACTN4 Antibody (C-term) in T47D, A2058, A375, HepG2 cell line and mouse bladder tissue lysates (35ug/lane). ACTN4 (arrow) was detected using the purified Pab.)
Related Product Information for anti-ACTN4 antibody
Alpha actinins belong to the spectrin superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This protein is a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and is thought to be involved in metastatic processes. Mutations in the gene encoding this protein have been associated with focal and segmental glomerulosclerosis.
Product Categories/Family for anti-ACTN4 antibody
References
Kikuchi,S., Clin. Cancer Res. 14 (17), 5348-5356 (2008)
Barbolina,M.V., Lab. Invest. 88 (6), 602-614 (2008)
Kimura,M., Intern. Med. 47 (12), 1099-1106 (2008)
Barbolina,M.V., Lab. Invest. 88 (6), 602-614 (2008)
Kimura,M., Intern. Med. 47 (12), 1099-1106 (2008)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
104854
NCBI Official Full Name
alpha-actinin-4
NCBI Official Synonym Full Names
actinin, alpha 4
NCBI Official Symbol
ACTN4
NCBI Official Synonym Symbols
FSGS; FSGS1; ACTININ-4
NCBI Protein Information
alpha-actinin-4
UniProt Protein Name
Alpha-actinin-4
UniProt Gene Name
ACTN4
UniProt Entry Name
ACTN4_HUMAN
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Product Notes
The ACTN4 actn4 (Catalog #AAA286904) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 785-813. The ACTN4 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ACTN4 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the ACTN4 actn4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ACTN4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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