IHC (Immunohiostchemistry)
(Tissue: rat brain tissue; 4% Paraformaldehyde-fixed and paraffinembedded; Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37 degrees C for 20 min; Incubation: Anti-Aggrecanase-2/ADAMTS5 Polyclonal Antibody, Unconjugated 1:200, overnight at 4 degrees C, followed by conjugation to the secondary antibody and DAB staining.)
Rabbit ADAMTS5 Polyclonal Antibody | anti-ADAMTS5 antibody
ADAMTS5
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity purified by Protein A
Synonyms
ADAMTS5, Antibody; ADAMTS5; A disintegrin and metalloproteinase with thrombospondin motifs 5; A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 5; A Disintigrin And Metalloproteinase with ThromboSpondin motif-5; ADAM metallopeptidase with thrombospondin type 1 motif 5; ADAM TS 11; ADAM TS 5; ADAM TS5; ADAMTS 11; ADAMTS 5; ADAMTS11; ADMP 2; ADMP2; Aggrecanase 2; aggrecanase-2; FLJ36738; Implantin; ThromboSpondin motif-5; anti-ADAMTS5 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purified by Protein A
Form/Format
0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Applicable Applications for anti-ADAMTS5 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
KLH conjugated synthetic peptide derived from human Aggrecan 2 511-610/930
Positive Control
Expressed at low level in placenta primarily but also detected in heart and brain, cervix,uterus, bladder, esophagus, rib cartilage, chondroblastoma, fibrous tissue and a joint capsule from an arthritic patient.
Subcellular Location
Secreted, extracellular space, extracellular matrix.
Preparation and Storage
Store at -20 degree C for one year. Avoid repeated freeze/thaw cycles.
The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20 degree C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 degree C.
The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20 degree C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 degree C.
IHC (Immunohiostchemistry)
(Tissue: rat brain tissue; 4% Paraformaldehyde-fixed and paraffinembedded; Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37 degrees C for 20 min; Incubation: Anti-Aggrecanase-2/ADAMTS5 Polyclonal Antibody, Unconjugated 1:200, overnight at 4 degrees C, followed by conjugation to the secondary antibody and DAB staining.)
IHC (Immunohiostchemistry)
(Tissue: rat brain tissue; 4% Paraformaldehyde-fixed and paraffinembedded; Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37 degrees C for 20 min; Incubation: Anti-Aggrecanase-2/ADAMTS5 Polyclonal Antibody, Unconjugated 1:200, overnight at 4 degrees C, followed by conjugation to the secondary antibody and DAB staining.)
WB (Western Blot)
(Sample: Lane 1: Mouse Placenta tissue lysates.Lane 2: Mouse Spleen tissue lysates.Lane 3: Human U87MG cell lysates.Lane 4: Human U251 cell lysates.Lane 5: Human SH-SY5Y cell lysates.Lane 6: Human HeLa cell lysates.Primary: Anti- ADAMTS5 at 1/1000 dilution.Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution.Predicted band size: 102 kDa.Observed band size: 102 kDa.)
WB (Western Blot)
(Sample: Lane 1: Mouse Placenta tissue lysates.Lane 2: Mouse Spleen tissue lysates.Lane 3: Human U87MG cell lysates.Lane 4: Human U251 cell lysates.Lane 5: Human SH-SY5Y cell lysates.Lane 6: Human HeLa cell lysates.Primary: Anti- ADAMTS5 at 1/1000 dilution.Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution.Predicted band size: 102 kDa.Observed band size: 102 kDa.)
Related Product Information for anti-ADAMTS5 antibody
Metalloproteinase that plays an important role in connective tissue organization, development, inflammation, arthritis, and cell migration. ADAMTS5 is an extracellular matrix (ECM) degrading enzyme that show proteolytic activity toward the hyalectan group of chondroitin sulfate proteoglycans (CSPGs) including aggrecan, versican, brevican and neurocan (PubMed:16133547, PubMed:18992360). Cleavage within the hyalectans occurs at Glu-Xaa recognition motifs. Plays a role in embryonic development, including limb and cardiac morphogenesis, and skeletal muscle development through its versican remodeling properties. Participates in development of brown adipose tissue and browning of white adipose tissue. Plays an important role for T-lymphocyte migration from draining lymph nodes following viral infection.
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
102 kDa
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Product Notes
The ADAMTS5 (Catalog #AAA268970) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ADAMTS5 reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ADAMTS5 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the ADAMTS5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ADAMTS5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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