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product-image-AAA212933_WB13.jpg WB (Western Blot) (Detection of human Adenylosuccinate Lyase/ADSL by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Adenylosuccinate Lyase/ADSL antibody AAA212933 (lot AAA212933-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)

Rabbit anti-Human Adenylosuccinate Lyase/ADSL Polyclonal Antibody | anti-ADSL antibody

Rabbit anti-Adenylosuccinate Lyase/ADSL Antibody, Affinity Purified

Gene Names
ADSL; ASL; AMPS; ASASE
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
Adenylosuccinate Lyase/ADSL, Antibody; Rabbit anti-Adenylosuccinate Lyase/ADSL Antibody, Affinity Purified; adenylosuccinase; adenylosuccinate lyase; ADSL; AMPS; ASASE; ASL; Adenylosuccinate lyase; anti-ADSL antibody
Ordering
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-ADSL antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 200 and 250
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human Adenylosuccinate Lyase/ADSL by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Adenylosuccinate Lyase/ADSL antibody AAA212933 (lot AAA212933-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)

product-image-AAA212933_WB13.jpg WB (Western Blot) (Detection of human Adenylosuccinate Lyase/ADSL by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Adenylosuccinate Lyase/ADSL antibody AAA212933 (lot AAA212933-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)

IP (Immunoprecipitation)

(Detection of human Adenylosuccinate Lyase/ADSL by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Adenylosuccinate Lyase/ADSL antibody AAA212933 (lot AAA212933-1) used for IP at 6 ug per reaction. Adenylosuccinate Lyase/ADSL was also immunoprecipitated by rabbit anti-Adenylosuccinate Lyase/ADSL antibody BL18708. For blotting immunoprecipitated Adenylosuccinate Lyase/ADSL, AAA212933 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)

product-image-AAA212933_IP15.jpg IP (Immunoprecipitation) (Detection of human Adenylosuccinate Lyase/ADSL by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Adenylosuccinate Lyase/ADSL antibody AAA212933 (lot AAA212933-1) used for IP at 6 ug per reaction. Adenylosuccinate Lyase/ADSL was also immunoprecipitated by rabbit anti-Adenylosuccinate Lyase/ADSL antibody BL18708. For blotting immunoprecipitated Adenylosuccinate Lyase/ADSL, AAA212933 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Related Product Information for anti-ADSL antibody
Adenylosuccinate Lyase /ADSL is involved in both de novo synthesis of purines and formation of adenosine monophosphate from inosine monophosphate. It catalyzes two reactions in AMP biosynthesis: the removal of a fumarate from succinylaminoimidazole carboxamide (SAICA) ribotide to give aminoimidazole carboxamide ribotide (AICA) and removal of fumarate from adenylosuccinate to give AMP. Adenylosuccinase deficiency results in succinylpurinemic autism, psychomotor retardation, and, in some cases, growth retardation associated with muscle wasting and epilepsy [taken from NCBI Entrez Gene (Gene ID: 158)].
Product Categories/Family for anti-ADSL antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
158
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
48,328 Da
NCBI Official Full Name
adenylosuccinate lyase isoform a
NCBI Official Synonym Full Names
adenylosuccinate lyase
NCBI Official Symbol
ADSL
NCBI Official Synonym Symbols
ASL; AMPS; ASASE
NCBI Protein Information
adenylosuccinate lyase; adenylosuccinase
UniProt Protein Name
Adenylosuccinate lyase
UniProt Gene Name
ADSL
UniProt Synonym Gene Names
AMPS; ASL; ASase
UniProt Entry Name
PUR8_HUMAN

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Product Notes

The ADSL adsl (Catalog #AAA212933) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Adenylosuccinate Lyase/ADSL Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Adenylosuccinate Lyase/ADSL can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the ADSL adsl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Adenylosuccinate Lyase/ADSL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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