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FCM/FACS (Flow Cytometry)
(ADH4 Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human, mouse ADH4 Polyclonal Antibody | anti-ADH4 antibody
ADH4 Antibody (C-term)
Gene Names
ADH4; ADH-2; HEL-S-4
Reactivity
Human, mouse
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ADH4, Antibody; ADH4 Antibody (C-term); Alcohol dehydrogenase 4; Alcohol dehydrogenase class II pi chain; ADH4; anti-ADH4 antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ADH4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 319-348 amino acids from the C-terminal region of human ADH4.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.48 (varies by lot)
Sequence Positions
319-348
Sequence Length
399
Applicable Applications for anti-ADH4 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(ADH4 Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(ADH4 Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohistochemisry)
(ADH4 antibody(C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ADH4 antibody(C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(ADH4 antibody(C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ADH4 antibody(C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of ADH4 (arrow) using rabbit polyclonal ADH4 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ADH4 gene.)
WB (Western Blot)
(Western blot analysis of ADH4 (arrow) using rabbit polyclonal ADH4 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ADH4 gene.)
WB (Western Blot)
(ADH4 Antibody (C-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the ADH4 antibody detected the ADH4 protein (arrow).)
WB (Western Blot)
(ADH4 Antibody (C-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the ADH4 antibody detected the ADH4 protein (arrow).)
Related Product Information for anti-ADH4 antibody
This gene encodes class II alcohol dehydrogenase 4 pi
subunit, which is a member of the alcohol dehydrogenase family.
Members of this enzyme family metabolize a wide variety of
substrates, including ethanol, retinol, other aliphatic alcohols,
hydroxysteroids, and lipid peroxidation products. Class II alcohol
dehydrogenase is a homodimer composed of 2 pi subunits. It exhibits
a high activity for oxidation of long-chain aliphatic alcohols and
aromatic alcohols and is less sensitive to pyrazole. This gene is
localized to chromosome 4 in the cluster of alcohol dehydrogenase
genes.
subunit, which is a member of the alcohol dehydrogenase family.
Members of this enzyme family metabolize a wide variety of
substrates, including ethanol, retinol, other aliphatic alcohols,
hydroxysteroids, and lipid peroxidation products. Class II alcohol
dehydrogenase is a homodimer composed of 2 pi subunits. It exhibits
a high activity for oxidation of long-chain aliphatic alcohols and
aromatic alcohols and is less sensitive to pyrazole. This gene is
localized to chromosome 4 in the cluster of alcohol dehydrogenase
genes.
Product Categories/Family for anti-ADH4 antibody
References
Pochareddy, S., et al. Gene 460 (1-2), 1-7 (2010) :
Preuss, U.W., et al. Addict Biol (2010) In press :
Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010)
Rainero, I., et al. Headache 50(1):92-98(2010)
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :
Preuss, U.W., et al. Addict Biol (2010) In press :
Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010)
Rainero, I., et al. Headache 50(1):92-98(2010)
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40222
NCBI Official Full Name
alcohol dehydrogenase 4 isoform 2
NCBI Official Synonym Full Names
alcohol dehydrogenase 4 (class II), pi polypeptide
NCBI Official Symbol
ADH4
NCBI Official Synonym Symbols
ADH-2; HEL-S-4
NCBI Protein Information
alcohol dehydrogenase 4
UniProt Protein Name
Alcohol dehydrogenase 4
UniProt Gene Name
ADH4
UniProt Entry Name
ADH4_HUMAN
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Product Notes
The ADH4 adh4 (Catalog #AAA285303) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 319-348. The ADH4 Antibody (C-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ADH4 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the ADH4 adh4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ADH4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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