WB (Western Blot)
(Detection of mouse ADK by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ADK antibody (AAA212593 lot 2) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Rabbit anti-Human, Mouse ADK Polyclonal Antibody | anti-ADK antibody
Rabbit anti-ADK Antibody, Affinity Purified
Gene Names
ADK; AK
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
ADK, Antibody; Rabbit anti-ADK Antibody, Affinity Purified; testicular tissue protein Li 14; AK; adenosine 5'-phosphotransferase; adenosine kinase; Adenosine kinase; anti-ADK antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-ADK antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat
Immunogen
Between 312 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of mouse ADK by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ADK antibody (AAA212593 lot 2) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of mouse ADK by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ADK antibody (AAA212593 lot 2) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of human ADK by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, LNCaP, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ADK antibody (AAA212593 lot 2) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of human ADK by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, LNCaP, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ADK antibody (AAA212593 lot 2) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human ADK by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ADK antibody (AAA212593 lot 2) used for IP at 6 ug per reaction. ADK was also immunoprecipitated by a previous lot of this antibody (AAA212593 lot 1) and a second antibody against a different epitope of ADK (BL15848). For blotting immunoprecipitated ADK, AAA212593 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human ADK by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ADK antibody (AAA212593 lot 2) used for IP at 6 ug per reaction. ADK was also immunoprecipitated by a previous lot of this antibody (AAA212593 lot 1) and a second antibody against a different epitope of ADK (BL15848). For blotting immunoprecipitated ADK, AAA212593 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Related Product Information for anti-ADK antibody
Adenosine kinase (ADK) is an enzyme which catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents [taken from NCBI Entrez Gene (Gene ID: 132)].
Product Categories/Family for anti-ADK antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36,580 Da
NCBI Official Full Name
adenosine kinase isoform b
NCBI Official Synonym Full Names
adenosine kinase
NCBI Official Symbol
ADK
NCBI Official Synonym Symbols
AK
NCBI Protein Information
adenosine kinase; adenosine 5'-phosphotransferase
UniProt Protein Name
Adenosine kinase
UniProt Gene Name
ADK
UniProt Synonym Gene Names
AK
UniProt Entry Name
ADK_HUMAN
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Product Notes
The ADK adk (Catalog #AAA212593) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-ADK Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ADK can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the ADK adk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ADK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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