WB (Western Blot)
(Detection of human AE2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-AE2 antibody AAA212731 (lot AAA212731-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human AE2 Polyclonal Antibody | anti-AE2 antibody
Rabbit anti-AE2 Antibody, Affinity Purified
Gene Names
SLC4A2; AE2; HKB3; BND3L; NBND3; EPB3L1
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
AE2, Antibody; Rabbit anti-AE2 Antibody, Affinity Purified; anion exchanger 2 type b2; solute carrier family 4 (anion exchanger), member 2; non-erythroid band 3-like protein; NBND3; HKB3; erythrocyte membrane protein band 3-like 1; Solute carrier family 4 member 2; anion exchanger 2 type b1; anion exchanger 2 type a; Anion exchanger 2; anion exchange protein 2; AE2; AE 2; EPB3L1; BND3L; Anion exchange protein 2; solute carrier family 4 member 2; anti-AE2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-AE2 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Orangutan
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human AE2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-AE2 antibody AAA212731 (lot AAA212731-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human AE2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-AE2 antibody AAA212731 (lot AAA212731-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human AE2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-AE2 antibody AAA212731 (lot AAA212731-1) used for IP at 6 ug per reaction. AE2 was also immunoprecipitated by rabbit anti-AE2 antibody For blotting immunoprecipitated AE2, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human AE2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-AE2 antibody AAA212731 (lot AAA212731-1) used for IP at 6 ug per reaction. AE2 was also immunoprecipitated by rabbit anti-AE2 antibody For blotting immunoprecipitated AE2, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemistry)
(Detection of human AE2 by immunohistochemistry. Samples: FFPE serial sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-AE2 (Cat. No. AAA212731 Lot1, left image and Cat. No. Lot1, right image) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human AE2 by immunohistochemistry. Samples: FFPE serial sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-AE2 (Cat. No. AAA212731 Lot1, left image and Cat. No. Lot1, right image) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-AE2 antibody
Anion exchange protein 2 (AE2) is a plasma membrane anion exchange protein of wide distribution [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P04920].
Product Categories/Family for anti-AE2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
137,009 Da
NCBI Official Full Name
anion exchange protein 2 isoform 1
NCBI Official Synonym Full Names
solute carrier family 4 (anion exchanger), member 2
NCBI Official Symbol
SLC4A2
NCBI Official Synonym Symbols
AE2; HKB3; BND3L; NBND3; EPB3L1
NCBI Protein Information
anion exchange protein 2; AE 2; anion exchanger 2 type a; anion exchanger 2 type b1; anion exchanger 2 type b2; solute carrier family 4 member 2; non-erythroid band 3-like protein; erythrocyte membrane protein band 3-like 1
UniProt Protein Name
Anion exchange protein 2
UniProt Gene Name
SLC4A2
UniProt Synonym Gene Names
AE2; EPB3L1; HKB3; MPB3L; AE 2; Anion exchanger 2; BND3L
UniProt Entry Name
B3A2_HUMAN
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Product Notes
The AE2 slc4a2 (Catalog #AAA212731) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-AE2 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AE2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the AE2 slc4a2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AE2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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