WB (Western Blot)
(All lanes : Anti-AHR Antibody (Center) at 1:500 dilutionLane 1: Hela whole cell lysateLane 2: MCF-7 whole cell lysateLane 3: A549 whole cell lysateLane 4: PC-3 whole cell lysateLane 5: HepG2 whole cell lysateLane 6: C2C12 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 96 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human, mouse AHR Polyclonal Antibody | anti-AHR antibody
AHR Antibody (Center)
Gene Names
AHR; bHLHe76
Reactivity
Human, mouse
Applications
ELISA, Western Blot
Synonyms
AHR, Antibody; AHR Antibody (Center); Aryl hydrocarbon receptor; Ah receptor; AhR; Class E basic helix-loop-helix protein 76; bHLHe76; AHR; BHLHE76; anti-AHR antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This AHR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 555-582 amino acids from the Central region of human AHR.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
555-582
Sequence Length
848
Applicable Applications for anti-AHR antibody
ELISA, WB (Western Blot)
Function
Ligand-activated transcriptional activator.Binds to the XRE promoter region of genes itactivates (PubMed: , PubMed:28602820,PubMed: , PubMed:23275542, PubMed:30373764). Activates the expression of multiple phase I and II xenobiotic chemical metabolizing enzyme genes (such as the CYP1A1 gene) (PubMed: ). Mediates biochemical and toxic effects of halogenated aromatic hydrocarbons (PubMed: ). Involved in cell-cycle regulation (PubMed: ). Likely to play an important role in the development and maturation of many tissues (PubMed:12213388). Regulates the circadian clock by inhibiting the basal and circadian expression of the corecircadian component PER1 (PubMed: ). Inhibits PER1 by repressing the CLOCK-ARNTL/BMAL1 heterodimer mediated transcriptional activation of PER1. The heterodimer ARNT:AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription (PubMed: ).
Cellular Location
Cytoplasm{ECO:0000250|UniProtKB:P30561}. Nucleus {ECO:0000250|UniProtKB:P30561}. Note=Initially cytoplasmic; upon binding with ligand and interaction with a HSP90, it translocates to the nucleus{ECO:0000250|UniProtKB:P30561}
Tissue Location
Expressed in all tissues tested including blood, brain, heart, kidney, liver, lung, pancreas and skeletal muscle.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(All lanes : Anti-AHR Antibody (Center) at 1:500 dilutionLane 1: Hela whole cell lysateLane 2: MCF-7 whole cell lysateLane 3: A549 whole cell lysateLane 4: PC-3 whole cell lysateLane 5: HepG2 whole cell lysateLane 6: C2C12 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 96 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-AHR Antibody (Center) at 1:500 dilutionLane 1: Hela whole cell lysateLane 2: MCF-7 whole cell lysateLane 3: A549 whole cell lysateLane 4: PC-3 whole cell lysateLane 5: HepG2 whole cell lysateLane 6: C2C12 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 96 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-AHR antibody
AHR is a ligand-activated transcription factor
involved in the regulation of biological responses to planar
aromatic hydrocarbons. This receptor has been shown to regulate
xenobiotic-metabolizing enzymes such as cytochrome P450. Its
ligands included a variety of aromatic hydrocarbons. [provided by
RefSeq].
involved in the regulation of biological responses to planar
aromatic hydrocarbons. This receptor has been shown to regulate
xenobiotic-metabolizing enzymes such as cytochrome P450. Its
ligands included a variety of aromatic hydrocarbons. [provided by
RefSeq].
References
Davila, S., et al. Genes Immun. (2010) In press :
Kalthoff, S., et al. J. Biol. Chem. 285(9):5993-6002(2010)
Hall, J.M., et al. Mol. Endocrinol. 24(2):359-369(2010)
Schroeder, J.C., et al. Biochemistry 49(2):393-400(2010)
Kalthoff, S., et al. J. Biol. Chem. 285(9):5993-6002(2010)
Hall, J.M., et al. Mol. Endocrinol. 24(2):359-369(2010)
Schroeder, J.C., et al. Biochemistry 49(2):393-400(2010)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
aryl hydrocarbon receptor
NCBI Official Synonym Full Names
aryl hydrocarbon receptor
NCBI Official Symbol
AHR
NCBI Official Synonym Symbols
bHLHe76
NCBI Protein Information
aryl hydrocarbon receptor
UniProt Protein Name
Aryl hydrocarbon receptor
UniProt Gene Name
AHR
UniProt Synonym Gene Names
BHLHE76; Ah receptor; AhR; bHLHe76
UniProt Entry Name
AHR_HUMAN
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Product Notes
The AHR ahr (Catalog #AAA283969) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 555-582. The AHR Antibody (Center) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's AHR can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the AHR ahr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AHR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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