WB (Western Blot)
(AIB1 antibody (pAb) tested by Western blot. Detection of AIB1 by Western blot. Lane 1: HeLa whole-cell extract (30 ug). Lane 2: Whole cell extract (30 ug) of MCF-7 cells. Both probed with AIB1 antibody at a 1:500 dilution.)
Rabbit anti-Human AIB1/SRC-3 Polyclonal Antibody | anti-AIB1/SRC-3 antibody
AIB1/SRC-3 antibody (pAb)
Reactivity
Human
Applications
Western Blot, Chromatin Immunoprecipitation, Immunoprecipitation
Purity
Affinity Purified
Synonyms
AIB1/SRC-3, Antibody; AIB1/SRC-3 antibody (pAb); Amplified in Breast Cancer 1; Steroid Receptor Coactivator-3; Nuclear Receptor Coactivator 3; SRC-3; NCOA3; anti-AIB1/SRC-3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Purified IgG in 70mM Tris (pH8), 105mM NaCl, 31mM glycine, 0.07mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-AIB1/SRC-3 antibody
WB (Western Blot), ChIP (Chromatin immunoprecipitation)
Immunogen
This AIB1 antibody was raised against a peptide derived from human AIB1/SRC-3.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
WB (Western Blot)
(AIB1 antibody (pAb) tested by Western blot. Detection of AIB1 by Western blot. Lane 1: HeLa whole-cell extract (30 ug). Lane 2: Whole cell extract (30 ug) of MCF-7 cells. Both probed with AIB1 antibody at a 1:500 dilution.)
WB (Western Blot)
(AIB1 antibody (pAb) tested by Western blot. Detection of AIB1 by Western blot. Lane 1: HeLa whole-cell extract (30 ug). Lane 2: Whole cell extract (30 ug) of MCF-7 cells. Both probed with AIB1 antibody at a 1:500 dilution.)
ChIP (Chromatin Immunoprecipitation)
(AIB1 antibody tested by ChIP analysis. Chromatin IP performed using the ChIP-IT Express Kit and MCF-7 chromatin (1.5 x 106 cell equivalents per ChIP) using 10 ul of AIB1 / SRC-3 antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the pS2 gene promoter. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.)
ChIP (Chromatin Immunoprecipitation)
(AIB1 antibody tested by ChIP analysis. Chromatin IP performed using the ChIP-IT Express Kit and MCF-7 chromatin (1.5 x 106 cell equivalents per ChIP) using 10 ul of AIB1 / SRC-3 antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the pS2 gene promoter. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.)
Related Product Information for anti-AIB1/SRC-3 antibody
Short Description: Our AIB1/SRC-3 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation and Western blot; it has been shown to react with Human samples.
Background: AIB1 (Amplified in Breast Cancer 1, Steroid Receptor Coactivator-3, Nuclear Receptor Coactivator 3, SRC-3, NCOA3) is a chromatin-associated protein that interacts with nuclear hormone receptors to modify their activity as transcriptional activators. AIB1 has histone acetyltransferase (HAT) activity and recruits p300/CBP-associated factor (PCAF) and CREB binding protein (CBP) as part of a multisubunit co-activator complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. It is Involved in the coactivation of different nuclear receptors, such as GR, ER, RARs, RXRs, TRs, VDR and PPARs. It is also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFkB1 protein.
Background: AIB1 (Amplified in Breast Cancer 1, Steroid Receptor Coactivator-3, Nuclear Receptor Coactivator 3, SRC-3, NCOA3) is a chromatin-associated protein that interacts with nuclear hormone receptors to modify their activity as transcriptional activators. AIB1 has histone acetyltransferase (HAT) activity and recruits p300/CBP-associated factor (PCAF) and CREB binding protein (CBP) as part of a multisubunit co-activator complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. It is Involved in the coactivation of different nuclear receptors, such as GR, ER, RARs, RXRs, TRs, VDR and PPARs. It is also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFkB1 protein.
Product Categories/Family for anti-AIB1/SRC-3 antibody
NCBI and Uniprot Product Information
Molecular Weight
160kDa
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Product Notes
The AIB1/SRC-3 (Catalog #AAA59931) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AIB1/SRC-3 antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AIB1/SRC-3 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ChIP (Chromatin immunoprecipitation). Researchers should empirically determine the suitability of the AIB1/SRC-3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AIB1/SRC-3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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