WB (Western Blot)
(Detection of human and mouse AIMP1/EMAPII by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-AIMP1/EMAPII antibody AAA212995 (lot AAA212995-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human, Mouse AIMP1/EMAPII/SCYE1 Polyclonal Antibody | anti-AIMP1/EMAPII/SCYE1 antibody
Rabbit anti-AIMP1/EMAPII/SCYE1 Antibody, Affinity Purified
Gene Names
AIMP1; p43; HLD3; EMAP2; SCYE1; EMAPII
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
AIMP1/EMAPII/SCYE1, Antibody; Rabbit anti-AIMP1/EMAPII/SCYE1 Antibody, Affinity Purified; HLD3; SCYE1; p43; multisynthase complex auxiliary component p43; endothelial-monocyte activating polypeptide II; endothelial monocyte-activating polypeptide 2; EMAPII; EMAP2; ARS-interacting multifunctional protein 1; aminoacyl tRNA synthase complex-interacting multifunctional protein 1; small inducible cytokine subfamily E, member 1 (endothelial monocyte-activating); multisynthetase complex auxiliary component p43; Aminoacyl tRNA synthase complex-interacting multifunctional protein 1; aminoacyl tRNA synthetase complex interacting multifunctional protein 1; anti-AIMP1/EMAPII/SCYE1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-AIMP1/EMAPII/SCYE1 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 262 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse AIMP1/EMAPII by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-AIMP1/EMAPII antibody AAA212995 (lot AAA212995-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human and mouse AIMP1/EMAPII by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-AIMP1/EMAPII antibody AAA212995 (lot AAA212995-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human AIMP1/EMAPII by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-AIMP1/EMAPII antibody AAA212995 (lot AAA212995-1) used for IP at 6 ug per reaction. AIMP1/EMAPII was also immunoprecipitated by rabbit anti-AIMP1/EMAPII antibody A304-894A. For blotting immunoprecipitated AIMP1/EMAPII, AAA212995 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human AIMP1/EMAPII by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-AIMP1/EMAPII antibody AAA212995 (lot AAA212995-1) used for IP at 6 ug per reaction. AIMP1/EMAPII was also immunoprecipitated by rabbit anti-AIMP1/EMAPII antibody A304-894A. For blotting immunoprecipitated AIMP1/EMAPII, AAA212995 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IHC (Immunohistochemistry)
(Detection of human AIMP1/EMAPII by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-AIMP1/EMAPII (Cat. No. AAA212995 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human AIMP1/EMAPII by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-AIMP1/EMAPII (Cat. No. AAA212995 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-AIMP1/EMAPII/SCYE1 antibody
Aminoacyl tRNA synthase complex-interacting multifunctional protein 1 (AIMP1) is a cytokine that is specifically induced by apoptosis, and it is involved in the control of angiogenesis, inflammation, and wound healing. The release of this cytokine renders the tumor-associated vasculature sensitive to tumor necrosis factor. The precursor protein is identical to the p43 subunit, which is associated with the multi-tRNA synthetase complex, and it modulates aminoacylation activity of tRNA synthetase in normal cells. This protein is also involved in the stimulation of inflammatory responses after proteolytic cleavage in tumor cell [taken from NCBI Entrez Gene (Gene ID: 9255)].
Product Categories/Family for anti-AIMP1/EMAPII/SCYE1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
37,039 Da
NCBI Official Full Name
Homo sapiens aminoacyl tRNA synthetase complex-interacting multifunctional protein 1 (AIMP1), transcript variant 1, mRNA
NCBI Official Synonym Full Names
aminoacyl tRNA synthetase complex-interacting multifunctional protein 1
NCBI Official Symbol
AIMP1
NCBI Official Synonym Symbols
p43; HLD3; EMAP2; SCYE1; EMAPII
NCBI Protein Information
aminoacyl tRNA synthase complex-interacting multifunctional protein 1; ARS-interacting multifunctional protein 1; endothelial monocyte-activating polypeptide 2; endothelial-monocyte activating polypeptide II; multisynthase complex auxiliary component p43;
UniProt Protein Name
Aminoacyl tRNA synthase complex-interacting multifunctional protein 1
UniProt Gene Name
AIMP1
UniProt Synonym Gene Names
EMAP2; SCYE1; EMAP-2; EMAP-II
UniProt Entry Name
AIMP1_HUMAN
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Product Notes
The AIMP1/EMAPII/SCYE1 aimp1 (Catalog #AAA212995) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-AIMP1/EMAPII/SCYE1 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's AIMP1/EMAPII/SCYE1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the AIMP1/EMAPII/SCYE1 aimp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AIMP1/EMAPII/SCYE1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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