IF (Immunofluorescence)
(AAA324437 staining A549 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Rabbit AKR1CL2 Polyclonal Antibody | anti-AKR1CL2 antibody
AKR1CL2 Antibody
Gene Names
AKR1E2; TAKR; hTSP; htAKR; AKRDC1; AKR1CL2; LoopADR
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
AKR1CL2, Antibody; AKR1CL2 Antibody; 1, 5-anhydro-D-fructose reductase; 5-anhydro-D-fructose reductase; AF reductase; AKCL2_HUMAN; AKR1CL2; Akr1e2; AKRDC1; Aldo keto reductase family 1 member C like protein 2; Aldo-keto reductase family 1 member C-like protein 2; Aldo-keto reductase family 1 member E2; HTSP; LoopADR; Testis specific protein; Testis-specific protein; anti-AKR1CL2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
AKR1CL2 antibody detects endogenous levels of total AKR1CL2
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
320
Applicable Applications for anti-AKR1CL2 antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
A synthesized peptide
Tissue Specificity
Specifically expressed in testis (PubMed:12604216, PubMed:15118078). Expressed in testicular germ cells and testis interstitial cells (PubMed:15118078).
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (92%), Rabbit (100%), Dog (85%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(AAA324437 staining A549 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
IF (Immunofluorescence)
(AAA324437 staining A549 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
WB (Western Blot)
(Western blot analysis of extracts from mouse liver, using AKR1CL2 antibody.)
WB (Western Blot)
(Western blot analysis of extracts from mouse liver, using AKR1CL2 antibody.)
WB (Western Blot)
(Western blot analysis of extracts from HepG2 cells, using AKR1CL2 antibody.)
WB (Western Blot)
(Western blot analysis of extracts from HepG2 cells, using AKR1CL2 antibody.)
Related Product Information for anti-AKR1CL2 antibody
Function: Catalyzes the NADPH-dependent reduction of 1, 5-anhydro-D-fructose (AF) to 1, 5-anhydro-D-glucitol (By similarity). Has low NADPH-dependent reductase activity towards 9, 10-phenanthrenequinone (in vitro) (PubMed:12604216, PubMed:15118078).
Subunit Structure: Monomer.
Similarity: Belongs to the aldo/keto reductase family.
Subunit Structure: Monomer.
Similarity: Belongs to the aldo/keto reductase family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 37 kD
Predicted: 37 kDa
Predicted: 37 kDa
NCBI Official Full Name
1,5-anhydro-D-fructose reductase isoform 1
NCBI Official Synonym Full Names
aldo-keto reductase family 1 member E2
NCBI Official Symbol
AKR1E2
NCBI Official Synonym Symbols
TAKR; hTSP; htAKR; AKRDC1; AKR1CL2; LoopADR
NCBI Protein Information
1,5-anhydro-D-fructose reductase
UniProt Protein Name
1,5-anhydro-D-fructose reductase
UniProt Gene Name
AKR1E2
UniProt Synonym Gene Names
AKR1CL2; AKRDC1; htAKR1 PublicationManual assertion based on opinion iniRef.5; hTSP
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Product Notes
The AKR1CL2 akr1e2 (Catalog #AAA324437) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AKR1CL2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's AKR1CL2 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the AKR1CL2 akr1e2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AKR1CL2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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