WB (Western Blot)
(Western blot analysis of extracts from mouse liver cells, using Akt (Phospho-Tyr326) antibody. The lane on the right is treated with the synthesized peptide.)
Rabbit Akt Polyclonal Antibody | anti-Akt antibody
Akt (Phospho-Tyr326) Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Synonyms
Akt, Antibody; Akt (Phospho-Tyr326) Antibody; AKT; AKT1 kinase; C-AKT; EC 2.7.11.1; PKB; PKB-alpha; Protein kinase B; RAC; RAC-PK-alpha; RAC-alpha serine/threonine kinase; kinase Akt1; anti-Akt antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of Akt only when phosphorylated at tyrosine 326.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
480
Applicable Applications for anti-Akt antibody
WB (Western Blot)
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Immunogen Type
peptide
Immunogen Description
Peptide sequence around phosphorylation site of tyrosine 326 (N-D-Y(p)-G-R) derived from Human Akt.
Modification
Phospho
Target Name
Akt
Preparation and Storage
Store at -20 degree C
WB (Western Blot)
(Western blot analysis of extracts from mouse liver cells, using Akt (Phospho-Tyr326) antibody. The lane on the right is treated with the synthesized peptide.)
WB (Western Blot)
(Western blot analysis of extracts from mouse liver cells, using Akt (Phospho-Tyr326) antibody. The lane on the right is treated with the synthesized peptide.)
Related Product Information for anti-Akt antibody
AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. Phosphorylation of PTPN1 at 'Ser-50' negatively modulates its phosphatase activity preventing dephosphorylation of the insulin receptor and the attenuation of insulin signaling.
Product Categories/Family for anti-Akt antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
SDS-PAGE MW: 60kd
NCBI Official Full Name
RAC-alpha serine/threonine-protein kinase
NCBI Official Synonym Full Names
v-akt murine thymoma viral oncogene homolog 1
NCBI Official Symbol
AKT1
NCBI Official Synonym Symbols
AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
NCBI Protein Information
RAC-alpha serine/threonine-protein kinase
UniProt Protein Name
RAC-alpha serine/threonine-protein kinase
UniProt Gene Name
AKT1
UniProt Synonym Gene Names
PKB; RAC; PKB; PKB alpha
UniProt Entry Name
AKT1_HUMAN
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Product Notes
The Akt akt1 (Catalog #AAA303130) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Akt (Phospho-Tyr326) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Akt can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the Akt akt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Akt, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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