IF (Immunofluorescence)
(Immunofluorescence of AKT1S1 in rat brain tissue with AKT1S1 antibody at 20 μg/ml.)
Rabbit AKT1S1 Polyclonal Antibody | anti-AKT1S1 antibody
AKT1S1 Antibody
Gene Names
AKT1S1; Lobe; PRAS40
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
Purity
AKT1S1 Antibody is affinity chromatography purified via peptide column.
Synonyms
AKT1S1, Antibody; AKT1S1 Antibody; AKT1S1; Lobe; PRAS40; Proline-rich AKT1 substrate 1; 40 kDa proline-rich AKT substrate; AKT1 substrate 1 (proline-rich); anti-AKT1S1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
AKT1S1 antibody is human, mouse and rat reactive. At least three isoforms of AKT1S1 are known to exist.
Purity/Purification
AKT1S1 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
276
Applicable Applications for anti-AKT1S1 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
Conjugate
Unconjugated
Immunogen
AKT1S1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human AKT1S1.
Buffer
AKT1S1 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
AKT1S1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.
IF (Immunofluorescence)
(Immunofluorescence of AKT1S1 in rat brain tissue with AKT1S1 antibody at 20 μg/ml.)
IF (Immunofluorescence)
(Immunofluorescence of AKT1S1 in rat brain tissue with AKT1S1 antibody at 20 μg/ml.)
IHC (Immunohiostchemistry)
(Immunohistochemistry of AKT1S1 in rat brain tissue with AKT1S1 antibody at 2.5 μg/ml.)
IHC (Immunohiostchemistry)
(Immunohistochemistry of AKT1S1 in rat brain tissue with AKT1S1 antibody at 2.5 μg/ml.)
WB (Western Blot)
(Western blot analysis of AKT1S1 in human brain tissue lysate with AKT1S1 antibody at (A) 1 and (B) 2 μg/mL.)
WB (Western Blot)
(Western blot analysis of AKT1S1 in human brain tissue lysate with AKT1S1 antibody at (A) 1 and (B) 2 μg/mL.)
Related Product Information for anti-AKT1S1 antibody
AKT1S1 Antibody: The Akt signaling pathway contributes to the regulation of apoptosis after a variety of cell death signals. AKT1S1, also known as PRAS40, is a proline-rich substrate of the kinase AKT1 and is thought to play a role in neuroprotection mediated by nerve growth factor (NGF) after transient focal cerebral ischemia (1). AKT1S1 is also a substrate and potential regulator of mammalian target of rapamycin (mTOR), a serine/threonine kinase that regulates cell growth and cell cycle, and a negative regulator of autophagy (2). Treatment with the insulin-like growth factor-1 (IGF1) can indusce the phosphorylation of AKT1S1 via the PI3K/AKT signaling pathway in PC12 cells (3).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 30 kDa
Observed: 28 kDa
Observed: 28 kDa
NCBI Official Full Name
proline-rich AKT1 substrate 1 isoform a
NCBI Official Synonym Full Names
AKT1 substrate 1 (proline-rich)
NCBI Official Symbol
AKT1S1
NCBI Official Synonym Symbols
Lobe; PRAS40
NCBI Protein Information
proline-rich AKT1 substrate 1; AKT1S1; 40 kDa proline-rich AKT substrate
UniProt Protein Name
Proline-rich AKT1 substrate 1
UniProt Gene Name
AKT1S1
UniProt Entry Name
AKTS1_HUMAN
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Product Notes
The AKT1S1 akt1s1 (Catalog #AAA40696) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AKT1S1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's AKT1S1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the AKT1S1 akt1s1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AKT1S1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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