WB (Western Blot)
(Western blot analysis of lysates from HeLa cells, using Phospho-AKT1S1/PRAS40-T246 Rabbit pAb at 1:400 dilution. HeLa cells were treated with Insulin (100 nM) at 37 degree C for 5 minutes after serum-starvation overnight.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 45s.)
Rabbit AKT1S1-T246 Polyclonal Antibody | anti-AKT1S1-T246 antibody
Phospho-AKT1S1-T246 pAb
Gene Names
AKT1S1; Lobe; PRAS40
Applications
ELISA, Western Blot
Purity
Affinity Purification
Synonyms
AKT1S1-T246, Antibody; Phospho-AKT1S1-T246 pAb; Lobe; PRAS40; anti-AKT1S1-T246 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Form/Format
PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3
Sequence Length
256
Applicable Applications for anti-AKT1S1-T246 antibody
ELISA, WB (Western Blot)
Immunogen
Synthetic peptide. This information is considered to be commercially sensitive.
Cross-Reactivity
Human
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
WB (Western Blot)
(Western blot analysis of lysates from HeLa cells, using Phospho-AKT1S1/PRAS40-T246 Rabbit pAb at 1:400 dilution. HeLa cells were treated with Insulin (100 nM) at 37 degree C for 5 minutes after serum-starvation overnight.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 45s.)
WB (Western Blot)
(Western blot analysis of lysates from HeLa cells, using Phospho-AKT1S1/PRAS40-T246 Rabbit pAb at 1:400 dilution. HeLa cells were treated with Insulin (100 nM) at 37 degree C for 5 minutes after serum-starvation overnight.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 45s.)
WB (Western Blot)
(Western blot analysis of extracts of HeLa cells, using Phospho-AKT1S1-T246 antibody at 1:2000 dilution. HeLa cells were treated by Insulin (0.01U/ml) for 15 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 90s.)
WB (Western Blot)
(Western blot analysis of extracts of HeLa cells, using Phospho-AKT1S1-T246 antibody at 1:2000 dilution. HeLa cells were treated by Insulin (0.01U/ml) for 15 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 90s.)
Related Product Information for anti-AKT1S1-T246 antibody
AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439])
Product Categories/Family for anti-AKT1S1-T246 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed MW: 40kDa
Calculated MW: 27kDa
Calculated MW: 27kDa
NCBI Official Full Name
proline-rich AKT1 substrate 1 isoform b
NCBI Official Synonym Full Names
AKT1 substrate 1
NCBI Official Symbol
AKT1S1
NCBI Official Synonym Symbols
Lobe; PRAS40
NCBI Protein Information
proline-rich AKT1 substrate 1
UniProt Protein Name
Proline-rich AKT1 substrate 1
UniProt Gene Name
AKT1S1
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Product Notes
The AKT1S1-T246 akt1s1 (Catalog #AAA281286) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's AKT1S1-T246 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the AKT1S1-T246 akt1s1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AKT1S1-T246, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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