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IP (Immunoprecipitation)
(Immunoprecipitating GPT in Mouse liver tissueLane 1: Rabbit control IgG instead of AAA26912 U in Mouse liver tissue. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: AAA26912 (8?g) + Mouse liver tissue (500?g)Lane 3: Mouse liver tissue (10?g))
Rabbit Alanine aminotransferase 1 Polyclonal Antibody | anti-GPT antibody
Rabbit anti-human Alanine aminotransferase 1 polyclonal Antibody
Gene Names
GPT; AAT1; ALT1; GPT1
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
Purity
>95%, Protein G purified
Synonyms
Alanine aminotransferase 1, Antibody; Rabbit anti-human Alanine aminotransferase 1 polyclonal Antibody; Alanine aminotransferase 1 (ALT1) (EC 2.6.1.2) (Glutamate pyruvate transaminase 1) (GPT 1) (Glutamic--alanine transaminase 1) (Glutamic--pyruvic transaminase 1), GPT, AAT1 GPT1; anti-GPT antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
496
Applicable Applications for anti-GPT antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
Recommended dilution:
WB:1:500-1:5000
IHC:1:200-1:500
IF: 1:50-1:200
IP:1:200-1:2000
WB:1:500-1:5000
IHC:1:200-1:500
IF: 1:50-1:200
IP:1:200-1:2000
Immunogen
Recombinant human Alanine aminotransferase 1 protein(2-496aa)
Immunogen Species
Homo sapiens (Human)
Research Area
Signal Transduction
Target Names
GPT
Conjugation
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-47020 / sc-47022
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C.
IP (Immunoprecipitation)
(Immunoprecipitating GPT in Mouse liver tissueLane 1: Rabbit control IgG instead of AAA26912 U in Mouse liver tissue. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: AAA26912 (8?g) + Mouse liver tissue (500?g)Lane 3: Mouse liver tissue (10?g))
IP (Immunoprecipitation)
(Immunoprecipitating GPT in Mouse liver tissueLane 1: Rabbit control IgG instead of AAA26912 U in Mouse liver tissue. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: AAA26912 (8?g) + Mouse liver tissue (500?g)Lane 3: Mouse liver tissue (10?g))
IHC (Immunohistochemistry)
(IHC image of AAA26912 diluted at 1:350 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohistochemistry)
(IHC image of AAA26912 diluted at 1:350 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohistochemistry)
(Immunofluorescent analysis of HepG2 cells using AAA26912 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IHC (Immunohistochemistry)
(Immunofluorescent analysis of HepG2 cells using AAA26912 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human kidney tissue using AAA26912 at dilution 1:100)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human kidney tissue using AAA26912 at dilution 1:100)
WB (Western Blot)
(Positive WB detected in: HepG2 whole cell lysate, Rat liver tissue, Mouse liver tissue All lanes: GPT antibody at 4.5?g/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 55 kDa Observed band size: 55 kDa)
WB (Western Blot)
(Positive WB detected in: HepG2 whole cell lysate, Rat liver tissue, Mouse liver tissue All lanes: GPT antibody at 4.5?g/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 55 kDa Observed band size: 55 kDa)
WB (Western Blot)
(Western blotAll lanes: Alanine aminotransferase 1 antibody at 20ug/mlLane 1: Rat heart tissueLane 2: Rat liver tissueSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 55 kDaObserved band size: 55 kDa)
WB (Western Blot)
(Western blotAll lanes: Alanine aminotransferase 1 antibody at 20ug/mlLane 1: Rat heart tissueLane 2: Rat liver tissueSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 55 kDaObserved band size: 55 kDa)
Related Product Information for anti-GPT antibody
This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
alanine aminotransferase 1
NCBI Official Synonym Full Names
glutamic-pyruvate transaminase (alanine aminotransferase)
NCBI Official Symbol
GPT
NCBI Official Synonym Symbols
AAT1; ALT1; GPT1
NCBI Protein Information
alanine aminotransferase 1
UniProt Protein Name
Alanine aminotransferase 1
UniProt Gene Name
GPT
UniProt Synonym Gene Names
AAT1; GPT1; ALT1; GPT 1
UniProt Entry Name
ALAT1_HUMAN
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Product Notes
The GPT gpt (Catalog #AAA26912) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Alanine aminotransferase 1 polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Alanine aminotransferase 1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP). Recommended dilution: WB:1:500-1:5000 IHC:1:200-1:500 IF: 1:50-1:200 IP:1:200-1:2000. Researchers should empirically determine the suitability of the GPT gpt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Alanine aminotransferase 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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