FCM/FACS (Flow Cytometry)
(ALDH18A1 Antibody (Center) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit ALDH18A1 Polyclonal Antibody | anti-ALDH18A1 antibody
ALDH18A1 Antibody (Center)
Gene Names
ALDH18A1; GSAS; P5CS; PYCS; ARCL3A
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ALDH18A1, Antibody; ALDH18A1 Antibody (Center); Delta-1-pyrroline-5-carboxylate synthase; P5CS; Aldehyde dehydrogenase family 18 member A1; Glutamate 5-kinase; GK; Gamma-glutamyl kinase; Gamma-glutamyl phosphate reductase; GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; ALDH18A1; GSAS; PYCS; anti-ALDH18A1 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ALDH18A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 176-205 amino acids from the Central region of human ALDH18A1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.42 (varies by lot)
Sequence Positions
176-205
Sequence Length
793
Applicable Applications for anti-ALDH18A1 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(ALDH18A1 Antibody (Center) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(ALDH18A1 Antibody (Center) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with ALDH18A1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with ALDH18A1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of ALDH18A1 Antibody (Center) in K562 cell line lysates (35ug/lane). ALDH18A1 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of ALDH18A1 Antibody (Center) in K562 cell line lysates (35ug/lane). ALDH18A1 (arrow) was detected using the purified Pab.)
Related Product Information for anti-ALDH18A1 antibody
ALDH18A1 is a member of the aldehyde dehydrogenase family and encodes a bifunctional ATP- and NADPH-dependent mitochondrial enzyme with both gamma-glutamyl kinase and gamma-glutamyl phosphate reductase activities. The encoded protein catalyzes the reduction of glutamate to delta1-pyrroline-5-carboxylate, a critical step in the de novo biosynthesis of proline, ornithine and arginine.
Product Categories/Family for anti-ALDH18A1 antibody
References
Hu,C.A., et.al., Amino Acids 35 (4), 665-672 (2008)
Bicknell,L.S., et.al., Eur. J. Hum. Genet. 16 (10), 1176-1186 (2008)
Bicknell,L.S., et.al., Eur. J. Hum. Genet. 16 (10), 1176-1186 (2008)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
87302
NCBI Official Full Name
delta-1-pyrroline-5-carboxylate synthase isoform 2
NCBI Official Synonym Full Names
aldehyde dehydrogenase 18 family, member A1
NCBI Official Symbol
ALDH18A1
NCBI Official Synonym Symbols
GSAS; P5CS; PYCS; ARCL3A
NCBI Protein Information
delta-1-pyrroline-5-carboxylate synthase
UniProt Protein Name
Delta-1-pyrroline-5-carboxylate synthase
UniProt Gene Name
ALDH18A1
UniProt Synonym Gene Names
GSAS; P5CS; PYCS; P5CS; GK; GPR
UniProt Entry Name
P5CS_HUMAN
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Product Notes
The ALDH18A1 aldh18a1 (Catalog #AAA288178) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 176-205. The ALDH18A1 Antibody (Center) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's ALDH18A1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the ALDH18A1 aldh18a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ALDH18A1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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