WB (Western Blot)
(Detection of mouse ALDH18A1/P5CS by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, CT26, CH27, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ALDH18A1/P5CS antibody AAA212945 Lot 1 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human, Mouse ALDH18A1/P5CS Polyclonal Antibody | anti-ALDH18A1/P5CS antibody
Rabbit anti-ALDH18A1/P5CS Antibody, Affinity Purified
Gene Names
ALDH18A1; GSAS; P5CS; PYCS; ADCL3; SPG9A; SPG9B; ARCL3A
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
ALDH18A1/P5CS, Antibody; Rabbit anti-ALDH18A1/P5CS Antibody, Affinity Purified; P5CS; Glutamyl-gamma-semialdehyde dehydrogenase; SPG9B; SPG9A; SPG9; Spastic paraplegia-9 (spastic paraparesis with amyotrophy, cataracts and gastroesophageal reflux); spastic paraplegia 9 (autosomal dominant); PYCS; GSAS; ARCL3A; pyrroline-5-carboxylate synthetase (glutamate gamma-semialdehyde synthetase); aldehyde dehydrogenase family 18 member A1; delta1-pyrroline-5-carboxlate synthetase; delta-1-pyrroline-5-carboxylate synthase; delta-1-pyrroline-5-carboxylate synthetase; Glutamate-5-semialdehyde dehydrogenase; Gamma-glutamyl kinase; ADCL3; Gamma-glutamyl phosphate reductase (GPR); Glutamate 5-kinase (GK); Delta-1-pyrroline-5-carboxylate synthase; aldehyde dehydrogenase 18 family member A1; anti-ALDH18A1/P5CS antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-ALDH18A1/P5CS antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Orangutan
Immunogen
Between 575 and 625
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of mouse ALDH18A1/P5CS by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, CT26, CH27, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ALDH18A1/P5CS antibody AAA212945 Lot 1 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of mouse ALDH18A1/P5CS by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, CT26, CH27, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ALDH18A1/P5CS antibody AAA212945 Lot 1 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human ALDH18A1/P5CS by western blot. Samples: Whole cell lysate (10 ug) from Hep-G2, MCF-7, HeLa, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ALDH18A1/P5CS antibody AAA212945 Lot 1 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human ALDH18A1/P5CS by western blot. Samples: Whole cell lysate (10 ug) from Hep-G2, MCF-7, HeLa, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ALDH18A1/P5CS antibody AAA212945 Lot 1 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human ALDH18A1/P5CS by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ALDH18A1/P5CS antibody AAA212945 (lot AAA212945-1) used for IP at 6 ug per reaction. ALDH18A1/P5CS was also immunoprecipitated by rabbit anti-ALDH18A1/P5CS antibody For blotting immunoprecipitated ALDH18A1/P5CS, AAA212945 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human ALDH18A1/P5CS by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ALDH18A1/P5CS antibody AAA212945 (lot AAA212945-1) used for IP at 6 ug per reaction. ALDH18A1/P5CS was also immunoprecipitated by rabbit anti-ALDH18A1/P5CS antibody For blotting immunoprecipitated ALDH18A1/P5CS, AAA212945 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Related Product Information for anti-ALDH18A1/P5CS antibody
ALDH18A1/P5CS is a bifunctional enzyme that converts glutamate to glutamate 5-semialdehyde, an intermediate in the biosynthesis of proline, ornithine and arginine [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P54886].
Product Categories/Family for anti-ALDH18A1/P5CS antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
87,089 Da
NCBI Official Full Name
delta-1-pyrroline-5-carboxylate synthase isoform 1
NCBI Official Synonym Full Names
aldehyde dehydrogenase 18 family member A1
NCBI Official Symbol
ALDH18A1
NCBI Official Synonym Symbols
GSAS; P5CS; PYCS; ADCL3; SPG9A; SPG9B; ARCL3A
NCBI Protein Information
delta-1-pyrroline-5-carboxylate synthase
UniProt Protein Name
Delta-1-pyrroline-5-carboxylate synthase
UniProt Gene Name
ALDH18A1
UniProt Synonym Gene Names
GSAS; P5CS; PYCS; P5CS; GK; GPR
UniProt Entry Name
P5CS_HUMAN
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Product Notes
The ALDH18A1/P5CS aldh18a1 (Catalog #AAA212945) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-ALDH18A1/P5CS Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ALDH18A1/P5CS can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the ALDH18A1/P5CS aldh18a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ALDH18A1/P5CS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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