WB (Western Blot)
(Detection of human ALIX by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ALIX antibody AAA212078 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. ALIX was also immunoprecipitated by rabbit anti-ALIX antibody A302-937A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).)
Rabbit anti-Human ALIX Polyclonal Antibody | anti-ALIX antibody
Rabbit anti-ALIX Antibody, Affinity Purified
Gene Names
PDCD6IP; AIP1; ALIX; HP95; DRIP4
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
ALIX, Antibody; Rabbit anti-ALIX Antibody, Affinity Purified; dopamine receptor interacting protein 4; ALG-2 interacting protein 1; programmed cell death 6-interacting protein; PDCD6-interacting protein; HP95; DRIP4; ALIX; AIP1; ALG-2-interacting protein 1; ALG-2-interacting protein X; apoptosis-linked gene 2-interacting protein X; Programmed cell death 6-interacting protein; programmed cell death 6 interacting protein; anti-ALIX antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-ALIX antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Mouse, Rat
Immunogen
Between 818 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human ALIX by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ALIX antibody AAA212078 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. ALIX was also immunoprecipitated by rabbit anti-ALIX antibody A302-937A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).)
WB (Western Blot)
(Detection of human ALIX by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ALIX antibody AAA212078 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. ALIX was also immunoprecipitated by rabbit anti-ALIX antibody A302-937A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).)
IHC (Immunohistochemistry)
(Detection of human ALIX by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ALIX (Cat. No. AAA212078 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human ALIX by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ALIX (Cat. No. AAA212078 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
Related Product Information for anti-ALIX antibody
ALIX (ALG-2 interacting protein X) is thought to participate in programmed cell death. Studies using mouse cells have shown that overexpression of ALIX can block apoptosis. ALIX has been shown to bind to the PDCD6 (programmed cell death 6 interacting protein), a protein required for apoptosis, in a calcium-dependent manner. ALIX also binds to endophilins, proteins that regulate membrane shape during endocytosis [taken from NCBI Entrez Gene (Gene ID: 10015].
Product Categories/Family for anti-ALIX antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,453 Da
NCBI Official Full Name
programmed cell death 6-interacting protein isoform 1
NCBI Official Synonym Full Names
programmed cell death 6 interacting protein
NCBI Official Symbol
PDCD6IP
NCBI Official Synonym Symbols
AIP1; ALIX; HP95; DRIP4
NCBI Protein Information
programmed cell death 6-interacting protein
UniProt Protein Name
Programmed cell death 6-interacting protein
UniProt Gene Name
PDCD6IP
UniProt Synonym Gene Names
AIP1; ALIX; KIAA1375; PDCD6-interacting protein
UniProt Entry Name
PDC6I_HUMAN
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Product Notes
The ALIX pdcd6ip (Catalog #AAA212078) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-ALIX Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ALIX can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the ALIX pdcd6ip for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ALIX, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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